1. Co-infection with Arsenophonus nasoniae and Orientia tsutsugamushi in a traveler.
- Author
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Edouard S, Subramanian G, Lefevre B, Dos Santos A, Pouedras P, Poinsignon Y, Mediannikov O, and Raoult D
- Subjects
- Adult, Animals, Antibodies, Bacterial immunology, Antigens, Bacterial immunology, Antigens, Bacterial isolation & purification, Asia, Southeastern, Bacterial Proteins genetics, Bacterial Proteins isolation & purification, Bacterial Proteins metabolism, Biopsy, Coinfection, Enterobacteriaceae genetics, Enterobacteriaceae immunology, Enterobacteriaceae Infections diagnosis, Enterobacteriaceae Infections microbiology, Female, France, Humans, Mice, Mice, Inbred BALB C, Orientia tsutsugamushi genetics, Orientia tsutsugamushi immunology, Polymerase Chain Reaction, Scrub Typhus diagnosis, Scrub Typhus microbiology, Skin pathology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Travel, Antibodies, Bacterial blood, Enterobacteriaceae isolation & purification, Enterobacteriaceae Infections complications, Orientia tsutsugamushi isolation & purification, Scrub Typhus complications, Skin microbiology
- Abstract
Here we report a case of co-infection with Orientia tsutsugamushi, the causative agent of scrub typhus, and Arsenophonus nasoniae in a woman with a rash and an eschar who returned from a trip to Southeast Asia. A. nasoniae was previously considered to be a secondary insect and tick endosymbiont of unknown pathogenicity in humans. We amplified both O. tsutsugamushi and A. nasoniae DNA from a skin eschar with qPCR, and a seroconversion for O. tsutsugamushi and A. nasoniae was observed with immunofluorescence assays and western blotting for this patient. And we used 2-D western blotting with an A. nasoniae antigen and polyclonal mouse anti-A. nasoniae antibodies produced in our laboratory to detect the specific antigenic A. nasoniae proteins.
- Published
- 2013
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