1. Isolation and sequence analysis of the complete H gene of canine distemper virus from domestic dogs in Henan Province, China.
- Author
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Liu Y, Liu C, Liu W, Wu H, Ding H, Cao Y, Spibey N, Wang L, He W, Hao L, Li X, and Tian K
- Subjects
- Animals, Asia, China, Dogs, Genotype, Phylogeny, Sequence Analysis, DNA methods, Distemper virology, Distemper Virus, Canine genetics, Distemper Virus, Canine isolation & purification, Hemagglutinins, Viral genetics
- Abstract
Eighteen canine distemper virus (CDV) isolates were obtained from clinical samples in Henan province, China, between 2012 and 2016. These viruses could not be recognized by 1A4, a monoclonal antibody specific for the H protein of CDV vaccine strains. The complete haemagglutinin (H) genes of all 18 isolates were sequenced, and phylogenetic analysis showed that they segregated into two clusters within the Asia-1 genotype. Moreover, the H genes of four viruses were found to lack a potential N-glycosylation site at position 309, which is the most conserved site within the Asia-1 genotype of CDV, and a novel potential N-glycosylation site (amino acids 517-519) was found in strain HL013, which has not been reported previously. These results will help in achieving a better understanding of the evolution of CDV in China.
- Published
- 2019
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