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Abstract: Background: Hepatitis B e antigen negative chronic hepatitis (e− CHB) with detectable levels of hepatitis B virus DNA (HBV DNA) in serum has been reported in cases from Asia. This study was undertaken to find out prevalence e−CHB and to correlate its presence with the clinical status and severity of the illness in cases of chronic liver disease in India. Methods: All patients of infective hepatitis, who were hepatitis B surface antigen (HBsAg) positive by enzyme-linked immunosorbent assay (ELISA), were evaluated with liver function tests and HBeAg and antiHBe antibody studies. Polymerase chain reaction (PCR) test was carried out to detect HBV DNA qualitatively. Result: Out of 2064 samples tested by ELISA, 429 (20.78%) were HBsAg positive. HBV DNA (qualitative) was performed on all 429 patients and 74 (17.2%) were HBV DNA positive. Of these only 42 (56.75%) tested positive for HBeAg. Overall, 8.3% of HBeAg negative patients (32/384) were viraemic with evidence of chronic liver disease/clinical cirrhosis and alteration of transaminase levels, while three cases (0.84%) HBeAg positive cases did not show presence of HBV DNA. Conclusion: This study shows e−CHB prevalence rate of 8.3% in patients with HBV infection in India. Since HBeAg negative patients had detectable levels of HBV DNA as seen in HBeAg positive patients, benefit of antiviral therapy should be given to them. Population studies on e− CHB cases are needed to determine its true prevalence, natural course and response to therapy. [Copyright &y& Elsevier]