1. Structural characterization and immunostimulatory activity of a glucan from Cyclina sinensis.
- Author
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Gong, Yue, Cao, Chunyang, Ai, Chunqing, Wen, Chengrong, Wang, Lilong, Zhao, Jun, Han, Yanhui, Song, Shuang, and Xiao, Hang
- Subjects
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MONOSACCHARIDES , *TERRITORIAL waters , *MOLECULAR weights , *GLYCOGEN , *SECRETION , *MACROPHAGES - Abstract
Cyclina sinensis is an edible clam widely distributed along the coastal waters of Asia. In the present study, a polysaccharide (CSP-1) isolated from C. sinensis was purified by a DEAE-Sepharose Fast Flow column, and it had an average molecular weight of 3.8 × 105 Da and a prevalent component monosaccharide of Glc. The results of methylation analysis and 1D/2D NMR indicated that CSP-1 was a glycogen constructed with α-1,4-Glc and branched at C-6 every 9 Glc residues. In addition, Cong red test suggests CSP-1 was not a helical conformation, and irregular and spherical lumps were observed by AFM. Moreover, CSP-1 was found to possess potent immunostimulatory activity on the basis of its significant abilities to enhance NO production and cytokines (TNF-α, IL-1β and IL-6) secretion in RAW 264.7 macrophages. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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