1. Protein expression patterns in hek-Blue™ - Cells treated with Clinacanthus nutans extracts.
- Author
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Yang, Shun-Kai, Leo, Teik-Kee, Mai, Chun-Wai, Chin, Swee-Yee, Lamasudin, Dhilia, Lim, Swee-Hua-Erin, Tan, Ngai-Paing, and Lai, Kok-Song
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REACTIVE oxygen species , *PROTEIN expression , *SODIUM dodecyl sulfate , *GEL electrophoresis , *EXTRACTS - Abstract
Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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