1. [Macrolide-resistant Streptococcus pneumoniae on the islands of Gran Canaria and Lanzarote (Spain): molecular mechanisms and serogroup relationships].
- Author
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Artiles F, Horcajada-Herrera I, Noguera-Catalán J, Alamo-Antúnez I, Bordes-Benítez A, and Lafarga-Capuz B
- Subjects
- Adolescent, Adult, Atlantic Islands epidemiology, Bacterial Proteins genetics, Child, Child, Preschool, Female, Genotype, Humans, Infant, Male, Membrane Proteins genetics, Phenotype, Pneumococcal Infections epidemiology, Serotyping, Spain epidemiology, Streptococcus pneumoniae classification, Streptococcus pneumoniae genetics, Streptococcus pneumoniae isolation & purification, Drug Resistance, Multiple, Bacterial genetics, Macrolides pharmacology, Pneumococcal Infections microbiology, Streptococcus pneumoniae drug effects
- Abstract
Background: Macrolide resistance in Streptococcus pneumoniae is coded by the ermB and mefA/E genes. The aim of this study was to determine the status of macrolide-resistance, the molecular mechanisms involved, the serogroup relationships, and the level of co-resistance in S. pneumoniae isolates from Gran Canaria and Lanzarote, in the Canary Islands, Spain., Methods: Macrolide resistance phenotypes were investigated in 261 S. pneumoniae clinical isolates over a two-year period (2004 and 2005). Genotypes were determined by PCR (detection of ermB and mefA/E genes)., Results: Overall macrolide resistance was 40.6% (106 isolates); 79.2% (84) of resistant isolates presented the MLSB phenotype (98.8% harbored the ermB gene), with a predominance of serogroup 19, and 20.8% (22) presented the M phenotype (77.3% displayed the mefA/E gene), all associated with serogroup 14. Worthy of note, the M phenotype was found in 8 invasive isolates from Lanzarote (80%) all from serogroup 14. The ermB and mefA/E genes were detected in 7 isolates belonging to serogroup 19. Absence of co-resistance was observed most frequently in serogroup 14 (66.7%). Co-resistance with penicillin G, tetracycline, and trimethoprim-sulfamethoxazole was associated with serogroup 19 (36.8%). Two isolates (0.8%) were resistant to telithromycin., Conclusion: The frequency of macrolide resistance mechanisms in the Canary Islands is different from that observed in the rest of Spain, particularly in Lanzarote, where 80% of isolates harbored the mefA/E gene and belonged to serogroup 14.
- Published
- 2007
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