1. Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.
- Author
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Atarashi, Ryuichiro, Satoh, Katsuya, Sano, Kazunori, Fuse, Takayuki, Yamaguchi, Naohiro, Ishibashi, Daisuke, Matsubara, Takehiro, Nakagaki, Takehiro, Yamanaka, Hitoki, Shirabe, Susumu, Yamada, Masahito, Mizusawa, Hidehiro, Kitamoto, Tetsuyuki, Klug, Genevieve, McGlade, Amelia, Collins, Steven J, and Nishida, Noriyuki
- Subjects
CEREBROSPINAL fluid ,MICROBIOLOGICAL assay ,CREUTZFELDT-Jakob disease ,PROTEINS ,PRIONS - Abstract
The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP
Sc ) has generated the potential for sensitive detection of prions. Here we developed a new PrPSc amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrPSc in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD. [ABSTRACT FROM AUTHOR]- Published
- 2011
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