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Foraminifera are a group of mostly marine protists with high taxonomic diversity. Species identification is often complex, as both morphological and molecular approaches can be challenging due to a lack of unique characters and reference sequences. An integrative approach combining state of the art morphological and molecular tools is therefore promising. In this study, we analysed large benthic Foraminifera of the genus Amphisorus from Western Australia and Indonesia. Based on previous findings on high morphological variability observed in the Soritidae and the discontinuous distribution of Amphisorus along the coast of western Australia, we expected to find multiple morphologically and genetically unique Amphisorus types. In order to gain detailed insights into the diversity of Amphisorus, we applied micro CT scanning and shotgun metagenomic sequencing. We identified four distinct morphotypes of Amphisorus, two each in Australia and Indonesia, and showed that each morphotype is a distinct genotype. Furthermore, metagenomics revealed the presence of three dinoflagellate symbiont clades. The most common symbiont was Fugacium Fr5, and we could show that its genotypes were mostly specific to Amphisorus morphotypes. Finally, we assembled the microbial taxa associated with the two Western Australian morphotypes, and analysed their microbial community composition. Even though each Amphisorus morphotype harboured distinct bacterial communities, sampling location had a stronger influence on bacterial community composition, and we infer that the prokaryotic community is primarily shaped by the microhabitat rather than host identity. The integrated approach combining analyses of host morphology and genetics, dinoflagellate symbionts, and associated microbes leads to the conclusion that we identified distinct, yet undescribed taxa of Amphisorus. We argue that the combination of morphological and molecular methods provides unprecedented insights into the diversity of foraminifera, which paves the way for a deeper understanding of their biodiversity, and facilitates future taxonomic and ecological work. [ABSTRACT FROM AUTHOR]

Abstract: The stratigraphy of fringing coral reef environments and platforms provides unique insights into reef development and evolution. This study used twelve sediment cores from three transects across a reef flat in Nelly Bay, Magnetic Island (NE Australia), to examine its development over the Holocene. The maximum thickness of the Holocene reefal material based on seismic and bore log data was around 5m and comparable to other fringing reefs in the Great Barrier Reef (GBR). Six different sedimentary facies were identified in the cores and two coincided with reef accretion: the lower muddy sand with coral rubble and the upper siliciclastic sand with coral rubble. Radiocarbon and U-series dates show that the Nelly Bay fringing reef initiated around 6300yr BP on a gently sloping, unconsolidated or weakly cemented Pleistocene alluvial sedimentary facies. The ages of four massive coral heads (range between 5790 and 6290 calibrated C-14 yr BP) were almost contemporaneous with reefal initiation indicating that conditions were favourable for reef growth around this time. Vertical accretion rates for the Nelly Bay fringing reef were, on average about 0.5mm/yr over the last 6000yr, although rates were as high as 5mm/yr during initiation. Average lateral accretion rates varied from 98 to 120mm/yr, which are comparable to rates of other fringing reefs in the region. The age structure of the lower muddy sedimentary facies was consistent with the classic seaward prograding model of fringing reef development. In contrast, the upper siliciclastic sand with coral rubble facies was much younger in age (<1100yr BP) than the models and appears to represent an erosional unconformity between the upper and lower reefal facies possibly linked to late Holocene sea-level fall. This younger facies extends laterally across the reef flat and has no obvious prograding symmetry. Our integrated approach, using multi-proxy analyses of sediment cores from the Nelly Bay reef flat, highlights the complex development of this reef which include changes in coral composition and rubble preservation, reef evolution in response to sea-level change and changes in reef accretion rates. [Copyright &y& Elsevier]