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Austria is inhabited by more than 80 species of native and non-native freshwater fishes. Despite considerable knowledge about Austrian fish species, the latest Red List of threatened species dates back 15 years and a systematic genetic inventory of Austria's fish species does not exist. To fulfill this deficit, we employed DNA barcoding to generate an up-to-date and comprehensive genetic reference database for Austrian fish species. In total, 639 newly generated cytochrome c oxidase subunit 1 (COI) sequences were added to the 377 existing records from the BOLD data base, to compile a near complete reference dataset. Standard sequence similarity analyses resulted in 83 distinct clusters almost perfectly reflecting the expected number of species in Austria. Mean intraspecific distances of 0.22% were significantly lower than distances to closest relatives, resulting in a pronounced barcoding gap and unique Barcode Index Numbers (BINs) for most of the species. Four cases of BIN sharing were detected, pointing to hybridization and/or recent divergence, whereas in Phoxinus spp., Gobio spp. and Barbatula barbatula intraspecific splits, multiple BINs and consequently cryptic diversity were observed. The overall high identification success and clear genetic separation of most of the species confirms the applicability and accuracy of genetic methods for bio-surveillance. Furthermore, the new DNA barcoding data pinpoints cases of taxonomic uncertainty, which need to be addressed in further detail, to more precisely assort genetic lineages and their local distribution ranges in a new National Red-List. [ABSTRACT FROM AUTHOR]

Translocations of Baltic whitefish ( Coregonus sp.) into Austrian Alpine lakes have created 'artificial hybrid zones', threatening the genetic integrity of native lineages. We evaluate the genetic structure of Coregonus in Austrian lakes and characterize hybridization and introgression between native and introduced lineages. Fifteen populations ( N = 747) were assessed for allelic variation at eight microsatellite loci and a reduced set ( N = 253) for variation across two mtDNA genes (cyt b and NADH-3). Bayesian approaches were used to estimate individual admixture proportions ( q-values) and classify genotypes as native, introduced or hybrids. q-value distributions varied among populations highlighting differential hybridization and introgression histories. Many lakes revealed a clear distinction between native and introduced genotypes despite hybridization, whereas some locations revealed hybrid swarms. Genetic structure among lakes was congruent with morphological divergence and novelty raising speculation of multiple taxa, including a population south of the Alps, outside the putative native range of Coregonus. Although statistically congruent with inferences based on nuclear markers, mitochondrial haplotype data was not diagnostic with respect to native and non-native lineages, supporting that the Alpine region was colonized post-glacially by an admixture of mtDNA lineages, which coalesce >1 Ma. Mechanisms promoting or eroding lineage isolation are discussed, as well as a high potential to conserve native Alpine lineages despite the extensive historical use of introduced Baltic stocks. [ABSTRACT FROM AUTHOR]

In order to obtain information on the repeatability of hydroacoustic estimates of abundance and biomass of the fish stocks in Stechlinsee (Germany) and Irrsee (Austria), we performed synchronised surveys in three expert teams using either two identical 120 kHz SIMRAD EY500 echosounders (Stechlinsee) or one such machine and a 200 kHz BioSonics DT6000 (Irrsee). At Stechlinsee, where the fish stock is dominated by Coregonus albula, the night surveys with vertical beaming were made along 12 transects with two boats, one following the other at a distance of approximately 300 m. Fish-density estimates from the two units were highly correlated, but the slope of the regression differed from the expected value of 1. The regression relating biomass estimates of the two expert teams also revealed a close correlation with the slope not being significantly different from unity. This pattern could be explained by the fact that the differences in abundance were due to small targets differentially encountered by the two teams and contributing only marginally to total fish biomass. Nevertheless, we found that the results of a single hydroacoustic survey can be reproduced reliably by an independent team using similar equipment. The comparison between different machines at Irrsee, where the fish stock is dominated by Coregonus lavaretus, was done at night with the two echosounders mounted on the same boat and running simultaneously without interference. The abundance estimates correlated significantly but more weakly than in the Stechlinsee exercise, although the echosounding systems differed in many technical specifications. Correlation of biomass estimates was found to be robust, with the slope of the regression not significantly different from unity. Hence, we found that simultaneous surveys by two expert teams using split-beam systems from completely independent manufacturers, differing in sound frequency, beam width and shape, pulse length, ping rate, acquisition software, and post-processing software, produce directly comparable biomass estimates. [Copyright &y& Elsevier]

Definitions and concepts relevant to the evaluation of the Ecological Integrity of lakes are discussed herein. Their application to Traunsee, a deep lake located in the Austrian Alps which is affected by wastes of salt- and soda-producing industries, is evaluated, based on 13 contributions published in this special issue of Water, Air, and Soil Pollution.: Focus. [ABSTRACT FROM AUTHOR]