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The study was conducted to investigate the effect of biofertilizer on wheat variety Zardana production at four locations of Balochistan. Biofertilizer (Azospirillum Al-Q + N 45 kg ha-1, P25O 30 kg ha-1) was used to substitute for half of the Nitrogen (N) and phosphorus (P) fertilizer application rates as compared to a high fertilizer treatment (N 90 kgha-1, P2O5 60 kgha-1). The high mineral fertilizer and biofertilizer + half mineral fertilizer treatments were compared with a control treatment (no fertilizer). Significant grain yield were measured at all sites, although, at all sites both fertilizer treatments apparently out yielded the control and the biofertilizer + half mineral fertilizer treatment out yielded the high fertilizer treatment by 220-1180 kgha-1. Fresh yields differed significantly at all sites. At Mastung field, both fertilizer treatments out yielded the control by 3000 kgha-1 and at Quetta (Aghbarg field-2) the biofertilizer + half mineral fertilizer treatment out yielded the others by over 3000 kgha-1. The results proved that the application of biofertilizer in combination with N 45kgha-1 and P2O5 30 kgha-1 increased fresh yield from 11% to 59% and suggested increases in grain yield of 20-46% as compared with control treatment. [ABSTRACT FROM AUTHOR]