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Abstract In a series of three experiments during 1998–99 and 1999–2000 at Gazipur, Bangladesh, the causes of segregation of Ogura cytoplasmic genetic male sterility in local cultivars of radish were studied. Male-sterile populations at the BC5 and BC6 generations were grown under a range of field temperatures for 2 years and the results on pollen fertility tests revealed that the expression of male sterility was not affected by temperature. Neither was a genotype–year interaction found. The unexpected segregation observed in the male-sterile backcross generations might be due to the presence of restorer alleles in the maintainer parents. [ABSTRACT FROM AUTHOR]