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Introduction: India is one of the largest countries which is inhabited by diverse populations of different tribes, castes, religious, and migrant groups. In forensic investigation, personal identification plays the greatest importance. Lip prints are considered as one such tool for personal identification. Study of lip prints is referred to as cheiloscopy and they are considered as unique in every individual just like fingerprints. Materials and Methods: Three hundred randomly selected specimens were taken in our study comprising of both the genders and of various regions from Odisha, West Bengal (WB) and Bihar was considered in the study. The age selected in our study ranged from 18 to 25 years comprising of 151 males and 149 females. Lip impression was made and viewed for different lip prints pattern under a magnifying glass of 10X. Results: There was a total of six lip print type identified in the present study. The type of lip print pattern found more in males was Type 2 and in females was Type 1. The type of lip print pattern found more in Bihar and WB was Type 2 and Odisha was Type 1. Conclusion: As the lip print is unique for every individual, Cheiloscopy is considered as a useful method of identification in forensic odontology. The study showed statistically significant difference in gender with a variation in geographical location. [ABSTRACT FROM AUTHOR]