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The Dongpu Depression, located in the southwest of the Bohai Bay Basin, east China, is enriched in natural gas with proven reserves of about 480 billion m 3 . However, their genesis and distribution are not completely clear. On the basis of molecular, stable carbon isotope and noble gas isotope (Ar) composition of a large amount of gas samples and combined with the geological background, this paper analyzed the genesis and migration features of Paleogene gas from major gas fields in the Dongpu depression. The results show that Paleogene gases in the Dongpu Depression are of thermogenic origin, and can be divided into coal-derived gas, oil-associated gas and their mixture. Furthermore, Natural gases from the E 2 s 3 L and E 2 s 3 M are predominantly oil-associated gas generated from E 2 s 3 source rocks, while those from E 2 s 4 , and E 2 s 3 U and E 2 s 2 in the Baimiao area are derived from the Carboniferous-Permian coaly source rocks. In addition, the coal-derived gas can also mix with the oil-associated gas, which can be called mixed coal-derived gas and oil-associated gas. Carbon isotope data suggest that gases of E 2 s 3 and most E 2 s 2 are predominately unaltered, while those of E 2 s 4 and several E 2 s 2 mostly from Baimiao area are altered and undergoing strong dissipation. The geographical variation in the gas composition can be attributed to the geochemical fraction caused by vertical migration through the basement faults for the coal-derived gas in E 2 s 4 and several E 2 s 3 U and E 2 s 2 in Baimiao area. While the oil-associated gas in E 2 s 3 L and E 2 s 3 M are resulted from the near-source or even in situ accumulation. [ABSTRACT FROM AUTHOR]