1. Cashew Tree Pollen: An Unknown Source of IgE-Reactive Molecules.
- Author
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Danella Figo D, De Amicis K, Neiva Santos de Aquino D, Pomiecinski F, Gadermaier G, Briza P, Eduardo Santos Galvão C, Bussador do Amaral J, de Oliveira Martins C, Fernandes Morato Castro F, Kalil J, and Souza Santos K
- Subjects
- Adolescent, Adult, Aged, Allergens chemistry, Animals, Antigens, Plant adverse effects, Antigens, Plant chemistry, Antigens, Plant genetics, Antigens, Plant immunology, Betula metabolism, Brazil, Carrier Proteins analysis, Carrier Proteins immunology, Child, Child, Preschool, Cross Reactions immunology, Dermatophagoides farinae, Dermatophagoides pteronyssinus, Female, Humans, Male, Plant Proteins adverse effects, Plant Proteins chemistry, Plant Proteins genetics, Plant Proteins immunology, Pollen genetics, Proteomics, Rhinitis, Allergic, Seasonal immunology, Skin Tests, Allergens immunology, Anacardium chemistry, Immunoglobulin E immunology, Pollen adverse effects, Pollen chemistry, Rhinitis, Allergic, Seasonal chemically induced
- Abstract
Pollinosis is sub-diagnosed and rarely studied in tropical countries. Cashew tree pollen has been reported as an allergen source although the knowledge of its immunoglobulin E (IgE)-reactive molecules is lacking. Therefore, this work aimed to identify IgE-reactive molecules and provide a proteomic profile of this pollen. From the 830 proteins identified by shotgun analysis, 163 were annotated to gene ontology, and a list of 39 proteins filtered for high confidence was submitted to the Allfam database where nine were assigned to allergenic families. Thus, 12 patients from the northeast of Brazil with persistent allergic rhinitis and aggravation of symptoms during cashew flowering season were selected. Using a 2D-based approach, we identified 20 IgE-reactive proteins, four already recognized as allergens, including a homolog of the birch isoflavone-reductase (Bet v 6). IgE-reactivity against the extract in native form was confirmed for five patients in ELISA, with three being positive for Bet v 6. Herein, we present a group of patients with rhinitis exposed to cashew tree pollen with the first description of IgE-binding proteins and a proteomic profile of the whole pollen. Cashew tree pollen is considered an important trigger of rhinitis symptoms in clinical practice in the northeast of Brazil, and the elucidation of its allergenic molecules can improve the diagnostics and treatment for allergic patients.
- Published
- 2019
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