1. Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli.
- Author
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Gaturaga I, Chahan B, Xuan X, Huang X, Liao M, Fukumoto S, Hirata H, Nishikawa Y, Takashima Y, Suzuki H, Fujisaki K, and Sugimoto C
- Subjects
- Animals, Antigens, Protozoan genetics, Antigens, Surface genetics, Blotting, Western veterinary, Brazil epidemiology, Cattle, Cattle Diseases epidemiology, Cattle Diseases parasitology, Coccidiosis diagnosis, Coccidiosis epidemiology, Electrophoresis, Polyacrylamide Gel veterinary, Enzyme-Linked Immunosorbent Assay methods, Escherichia coli genetics, Fluorescent Antibody Technique veterinary, Mice, Mice, Inbred BALB C, Protozoan Proteins genetics, Recombinant Proteins genetics, Recombinant Proteins immunology, Seroepidemiologic Studies, Antibodies, Protozoan blood, Antigens, Protozoan immunology, Antigens, Surface immunology, Cattle Diseases diagnosis, Coccidiosis veterinary, Enzyme-Linked Immunosorbent Assay veterinary, Neospora immunology, Protozoan Proteins immunology
- Abstract
The surface antigen 1-related sequence 2 of Neospora caninum (NcSRS2) is considered as an immunodominant antigen. In this study, the gene encoding truncated NcSRS2 (NcSRS2t) lacking an N-terminal signal peptide and C-terminal hydrophobic regions was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. ELISA could discriminate clearly between known N. caninum-positive and -negative sera from cattle. Field serum samples collected from cattle in Brazil were examined for the diagnosis of N. caninum infection using ELISA. Of the 197 samples analyzed, 64 (32.5%) samples were positive for antibodies to N. caninum. Of the 64 ELISA-positive samples, 58 (90.6%) were confirmed as positive by Western blot analysis with whole-parasite antigens. These results suggest that ELISA with recombinant NcSRS2t is an effective method for diagnosis of N. caninum infection in cattle.
- Published
- 2005
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