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This article examined the association between body satisfaction and sexual identification among lesbian and bisexual women, since these factors help to understand the cultural background of the objectification of female bodies in Latin cultures. Women who identify as lesbian (N = 239) and bisexual (N = 60) completed demographic data and measures of self-esteem, physical appearance perfectionism, lesbian and bisexual identity difficulties, and body satisfaction. We performed a three-stage hierarchical multiple regression to explore how variables relate to body satisfaction. The results suggest that self-esteem plays a key role, explaining 20.4% of the variance in body satisfaction. We discussed the psychosocial and cultural aspects involved in the relationship between the variables, and social and aesthetic pressures on women's bodies. This study contributes to discussions on psychosocial aspects associated with body satisfaction among Brazilian lesbian and bisexual women.