1. Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil.
- Author
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Rabahi MF, Conceição EC, de Paiva LO, Souto MVML, Sisco MC, de Waard J, de Souza Caldas PC, Fandinho F, Ramos JP, de Carvalho LD, Campos CED, Lima KVB, da Silva SP, Sharma A, Robledo J, Paez UAH, Duarte RS, da Silva MG, Gomes LL, Vasconcellos SEG, Uwezeye C, de Jong BC, Junqueira-Kipnis AP, and Suffys PN
- Subjects
- Brazil epidemiology, Genes, Bacterial, Genome, Bacterial, Genotype, Humans, Molecular Epidemiology, Molecular Typing, Mycobacterium tuberculosis isolation & purification, Phylogeny, Polymorphism, Single Nucleotide, RNA, Ribosomal, 16S, Tuberculosis, Pulmonary epidemiology, Mycobacterium tuberculosis classification, Mycobacterium tuberculosis genetics, Tuberculosis, Pulmonary diagnosis, Tuberculosis, Pulmonary microbiology
- Abstract
Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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