1. Brazilian red propolis reduces orange-complex periodontopathogens growing in multispecies biofilms.
- Author
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Miranda SLF, Damasceno JT, Faveri M, Figueiredo L, da Silva HD, Alencar SMA, Rosalen PL, Feres M, and Bueno-Silva B
- Subjects
- Anti-Bacterial Agents pharmacology, Brazil, Chlorhexidine pharmacology, Color, Biofilms drug effects, Propolis pharmacology
- Abstract
This study investigated the antimicrobial effects of the ethanolic extract of Brazilian red propolis (BRP) on multispecies biofilms. A seven-day-old subgingival biofilm with 32 species was grown in a Calgary device. Biofilms were treated with BRP (1,600, 800, 400 and 200 μg ml
-1 ) twice a day for 1 min, starting from day 3. Chlorhexidine (0.12%) and dilution-vehicle were used as positive and negative controls, respectively. On day 7, metabolic activity and the microbial composition of the biofilms by DNA-DNA hybridization were determined. The viability data were analyzed by one-way ANOVA followed by Tukey's post hoc , whereas the microbial composition data were transformed via BOX-COX and analyzed using Dunnett's post hoc . BRP (1,600 μg ml-1 ) decreased biofilm metabolic activity by 45%, with no significant difference from chlorhexidine-treated samples. BRP (1,600 μg ml-1 ) and chlorhexidine significantly reduced levels of 14 bacterial species compared to the vehicle control. Taken together, BRP showed promising antimicrobial properties which may be useful in periodontal disease control.- Published
- 2019
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