1. Genetic analysis of full-length genomes and subgenomic sequences of TT virus-like mini virus human isolates.
- Author
-
Biagini P, Gallian P, Attoui H, Touinssi M, Cantaloube JF, de Micco P, and de Lamballerie X
- Subjects
- Base Composition, Brazil epidemiology, Circoviridae Infections epidemiology, Circoviridae Infections virology, Circovirus chemistry, Conserved Sequence genetics, DNA Virus Infections epidemiology, DNA, Viral blood, DNA, Viral genetics, Feces virology, France epidemiology, Humans, Japan epidemiology, Leukocytes, Mononuclear virology, Open Reading Frames genetics, Phylogeny, Recombination, Genetic genetics, Saliva virology, Torque teno virus chemistry, Circovirus genetics, DNA Virus Infections virology, Genome, Viral, Torque teno virus genetics
- Abstract
The phylogenetic relationship between the complete genomic sequences of ten Japanese and one French isolate of TT virus-like mini virus (TLMV) was investigated. Analysis of the variability of the nucleotide sequences and the detection of signature patterns for overlapping genes suggested that ORFs 1 and 2 are probably functional. However, this was not the case for a putative third ORF, ORF3. Throughout the viral genome, several nucleotide or amino acid motifs that are conserved in circoviruses such as TT virus (TTV) and chicken anaemia virus were identified. Phylogenetic analysis distinguished three main groups of TLMV and allowed the identification of putative recombination breakpoints in the untranslated region. TLMV genomes were detected by PCR in the plasma of 38/50 French blood donors tested and were also identified in peripheral blood mononuclear cells, faeces and saliva. A phylogenetic study of 37 TLMV strains originating from France, Japan and Brazil showed that groupings were not related to geographical origin.
- Published
- 2001
- Full Text
- View/download PDF