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A total of 134 samples, consisting of 58 wheat flour, 40 instant noodle and 36 biscuits, were analysed for the presence of deoxynivalenol (DON). The samples were obtained from retail markets of the city of São Paulo during the period 2010–2014. DON was determined by high performance liquid chromatography with ultraviolet detection and immunoaffinity sample clean-up. Method validation followed international guidelines. The LOD and LOQ were 60 and 200 µg kg−1, respectively, considering the three different types of samples analysed. The lowest recovery found in this study was 91.8% with RSD 4.5% for instant noodles. DON was detected in 91.4%, 97.5% and 97.2% of samples wheat flour, instant noodles and biscuits, respectively, resulting in a total of 94.8% with levels ranging from LOD to 1720.0 µg kg−1. [ABSTRACT FROM AUTHOR]

Deoxynivalenol (DON) is a mycotoxin produced by Fusarium graminearum and its intake represents a severe risk to human and animal health. The objective of this study was to evaluate levels of DON in wheat (Triticum aestivum L.) flour from two representative locations of south Brazil. Experiments were carried out in Pato Branco (Paraná) and Coxilha (Rio Grande do Sul) in a randomized complete block design with three replicates. Levels of DON were measured by high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS). This mycotoxin was detected in 97% of samples, ranging from 200 to 4140 µ g kg-1. Only 17% of samples presented DON beyond of the maximum allowed by the Brazilian Health Surveillance Agency up to the year 2012; even though, Fusarium head blight (FHB) epidemics were slight low in the growing season that the study was performed. According to our knowledge, this is the first report showing genetic variability of Brazilian cultivars to DON contamination and some genotypes have potential to be exploited as a source of low accumulation of this toxin. [ABSTRACT FROM AUTHOR]