1. Antioxidative bioavailability of artepillin C in Brazilian propolis.
- Author
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Shimizu K, Ashida H, Matsuura Y, and Kanazawa K
- Subjects
- Anti-Infective Agents pharmacokinetics, Anti-Infective Agents pharmacology, Biological Availability, Brazil, Caco-2 Cells, Cell Line, Tumor drug effects, Cell Line, Tumor metabolism, Dose-Response Relationship, Drug, Humans, Propolis, Antioxidants pharmacokinetics, Antioxidants pharmacology, Liver Neoplasms metabolism, Liver Neoplasms pathology, Oxidative Stress drug effects, Phenylpropionates pharmacokinetics, Phenylpropionates pharmacology
- Abstract
Propolis has strong antioxidative activity. We investigated here whether this activity was available in intestinal Caco-2 and hepatic HepG2 cells. Phenolics in Brazilian propolis, extracted with ethyl acetate after the removal of resin and wax with 90% methanol, included artepillin C at 21 mmol/100 g, p-coumaric acid and cinnamic acid relatives 24mmol, kaempferol and its derivatives 9.4 mmol, naringenin 2.8 mmol, isosakuranetin 0.9 mmol, chrysin at 0.8 mmol/100 g, and several minor components. When the extract was added to the apical side of Caco-2 monolayers, artepillin C was specifically incorporated into the cells and released to the basolateral side mostly without conjugation. Then, artepillin C was added to HepG2 cells and exposed to reactive oxygens. Artepillin C prevented oxidative damage dose-dependently, and suppressed lipid peroxidation evaluated with thiobarbituric acid reactive substances by 16% and the formation of 8-hydroxy-2'-deoxyguanosine in DNA by 36% at a concentration of 20microM. Artepillin C is a bioavailable antioxidant.
- Published
- 2004
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