Chloroplasts have luminescent metabolites-chlorophyll being the most known one-whose fluorescence emission may be a useful tool to assess the physiological status of the plant. Some antioxidants (flavonoids and carotenoids), and byproducts of membrane rupture (lipofuscins) and chlorophyll degradation (pheophytins), are chloroplasts' fluorescent metabolites directly involved in plant response to environmental stressors and pollutants and may act as a biomarker of stress. Here we hypothesized that climatic variations and air pollutants induce alterations in the emission profile of chloroplasts' fluorescent metabolites in Tillandsia usneoides (Bromeliaceae). To test this hypothesis, an active biomonitoring study was performed during 2 years in five polluted sites located at the Metropolitan Region of Campinas (São Paulo State, Brazil), aiming to identify target chloroplasts' fluorescent metabolites acting as biomarkers of environmental stress. In situ identification and quantification of the intensity of the fluorescence emission from target metabolites (flavonoids, carotenoids, lipofuscins, and pheophytins) were performed by the observation of fresh leaf sections under confocal laser scanning microscopy. Changes in the profile of fluorescence emission were correlated with local climate and air pollution data. The fluorescence emissions of flavonoids and carotenoids varied seasonally, with significant influence of rainfall and NO 2 . Our results expand the use of T. usneoides as a bioindicator by using alterations in the fluorescence emission profile of chloroplast metabolites. This application may be especially interesting for NO 2 biomonitoring.