1. TLC and HPLC methods for the determination of plumbagin for the diagnosis of poisoning by Plumbago scandens L.
- Author
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Teixeira, Carlos José Rocha, dos Santos, Barbara Pereira, Saraiva, Carolina Julia Costa, Pedroza, Heloísa de Paula, Oloris, Silvia Catarina Salgado, and Soto-Blanco, Benito
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GRAPHITE , *PLUMBAGIN , *THIN layer chromatography , *HIGH performance liquid chromatography , *GUINEA grass , *RODENTICIDES , *ORNAMENTAL plants - Abstract
Plumbago scandens L. (Plumbaginaceae) occurs in all regions of Brazil. It has been described as toxic to cattle and goats. Caustic lesions in the upper digestive tract characterize poisoning. P. scandens contains a naphthoquinone named plumbagin, which presents high cytotoxic activity. Plumbago auriculata Lam., a widely used ornamental plant, is considered potentially toxic, but there is limited data about its toxicity. This work aimed to validate analytical methodologies for determining the levels of plumbagin in samples of leaves, stems, and rumen content to be used as an auxiliary chemical marker in the laboratory diagnosis of intoxication. One methodology used thin layer chromatography (TLC), and another used high-performance liquid chromatography (HPLC). The presence of palisade grass (Urochloa brizantha (Hochst. ex A.Rich.) R.D.Webster), Guinea grass (Megathyrsus maximus (Jacq.) B.K.Simon & S.W.L.Jacobs), corn silage, and rumen content did not interfere with plumbagin in the two methodologies. The TLC methodology generates qualitative results but is simple to implement and has a low cost. The HPLC methodology showed a limit of detection (LOD) of 0.01 μg/mL and a limit of quantification (LOQ) of 0.05 μg/mL. Leaf and stem samples of P. scandens evaluated showed high levels of plumbagin (0.261 ± 0.087 % and 0.327 ± 0.055 %, respectively). In contrast, leaves of P. auriculata did not show detectable levels of the toxin, and some stem samples showed low levels (up to 0.000114 %). Thus, these methodologies can be used to confirm or rule out the consumption of P. scandens in rumen content from animals suspected of poisoning. [Display omitted] • TLC and HPLC methods for detecting plumbagin from Plumbago scandens in rumen and plant samples were validated. • The leaves and stem of Plumbago scandens showed high levels of plumbagin. • Plumbago auriculata showed no detectable levels in the leaves and low levels in the steams. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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