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The article presents a study which examines the genetic variation of Loma salmonae in wild and farmed salmon host to determine its variation level and genetic variants into different population. The study compares the variation between the ribosomal RNA (rRNA) genes and elongation factor-1 alpha (EF-1α) gene sequences among salmon hosts including rainbow trout, Pacific salmon, and brook trout raised from countries including British Columbia, Prince Edward Island, and Chile. Result shows that both of the environment type shows a variant of S. fontinalis (SV), which is a separate species. Meanwhile, it reveals that freshwater samples in Chile have the greatest genetic variation among the samples, suggesting that greater population diversity can be found on these region.