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STEM-related educational outreach offers students enriching opportunities to become more familiar with science, in terms of how it relates to their daily lives and with respect to possible career paths that they might want to follow. At the same time, graduate student trainees providing that outreach act as important resources for elementary and high school teachers while they hone their teaching skills and build confidence in the classroom. In this paper, six graduate students and recent graduates share their experiences with a variety of outreach programs that link young people with science in both Canada and the United States.

Objective: To evaluate the process of implementation and impact of a front-of-pack labeling intervention on purchases of labeled food products. Participants: A convenience sample of 111 college students were recruited to complete a survey as they exited markets where the intervention was being implemented. Participants had to have purchased at least one consumable item. Methods: Intercept surveys and audits were conducted at three markets, two times each over a period of three weeks. Results: The audits indicated high implementation fidelity (82% overall). However, customer surveys revealed that only 42% noticed the front-of-pack sticker, and the majority (89%) did not purchase an item with the sticker. However, customers who noticed the sticker were significantly more likely to purchase an item with the sticker (p = 0.002). Conclusions: These findings provide preliminary evidence for the feasibility and effectiveness of a front-of-pack labeling intervention in a university market setting.

Research suggests that, to narrow the gender gap in engineering, we should focus on helping young girls identify with engineering both because gendered attitudes emerge around kindergarten and because identity is more predictive than performance on persistence in the field. This qualitative study sought to understand the impact of collaborative engineering design on the development of engineering identities in elementary-school students and compared the findings across gender. We focused on three tiers of collaboration embedded into the engineering design process: peer groups, role models, and shared goals. More specifically, the elementary students worked in small teams and partnered with undergraduate engineers to help design and build dancing robots that come together for a coordinated dance performance. We used ethnographic methods, including pre- and post-program student interviews, video-recorded program sessions, and documentation of student work, to investigate elementary students' engineering identities. Three themes emerged from our analysis. First, working with peers encouraged students who were initially uninterested in engineering, the majority of whom were girls, to join the program and helped them to engage in the activities. Second, partnering with engineer role models contributed to the elementary students' developing identities as engineers: The girls were most influenced by the personal bonds they formed, while the boys were most influenced by the technical skills they learned. Third, all girls and most boys preferred the idea of working toward a shared goal over competitive projects that, as described by the students, can cause bad feelings and hurt friendships. Our work supports and extends elementary engineering literature by considering the role of multiple tiers of collaboration in identity development in girls and boys. Our results suggest that engineering design programs that foster collaboration can help more students, especially more girls, engage in and identify with engineering, thereby contributing to the narrowing of the gender gap.

As many schools move toward a three-tier model that incorporates a Response to Intervention (RtI) service delivery model in the social, emotional, and behavioral domains, school psychologists may provide leadership. The decision-making process for filtering students through multiple tiers of support and intervention and examining change is an area where school psychologists are encouraged to apply their expertise regarding assessment and evidence-based interventions. This paper describes an implementation of behavioral and social-emotional RtI in an elementary school setting. Issues and challenges related to measurement of change (i.e., responsiveness to intervention) and identification of students for additional supports as well as emerging possibilities of incorporating qualitative information in the process are discussed. (Contains 1 figure, 1 footnote and 3 tables.)

Universal screening of emotional and behavioral problems among students warrants further consideration by school professionals. School-based universal screening may provide opportunities for early identification and intervention, ultimately preventing the development of more severe problems and promoting more positive outcomes in the future. The "Behavioral and Emotional Screening System" (BESS) is a contemporary screening instrument that may be used to identify risk for emotional and behavioral problems in students from preschool to high school. The purpose of the present study was to examine the concurrent validity of the BESS in elementary school settings. Specifically, this study examined the relation between BESS ratings and report-card outcomes (i.e., academic, behavioral, and engagement marks). The results supported the hypotheses that students' risk-level classifications were significantly related to school-based outcome criterions and that school-based outcome criterions were deemed to be effective discriminators of students' risk level classification. Limitations, future directions for research, and implications for practice are discussed herein. (Contains 4 tables.)

The increasing emphasis on school-based mental health services is likely to increase the demand for school psychologists to provide counseling with students. Providing counseling in the school context can be challenging, especially given time constraints and limited number of sessions. Solution-focused brief counseling (SFBC) is an approach that warrants consideration for use with children at school. This synthesis provides a brief overview of the extant scholarship regarding SFBC, describes the guidelines for implementing this approach, explores considerations and implications for school psychologists who use this approach to provide counseling services, and recommends future directions for scholarship. Lessons learned through a university and school collaboration to provide student support services are also included. (Contains 3 tables.)

The development of social and emotional competence is crucial for students. "Second Step" is a curriculum designed to promote prosocial development and prevent violence. The purpose of this study was to evaluate the effectiveness of implementing one unit of the Second Step curriculum (Impulse Control and Problem Solving). A controlled, repeated measures design was utilized to assess the level of change in knowledge of social-emotional skills. Results indicated that change was evident from pre- to post-test for third and fourth grade students (N=149). Third grade students receiving the intervention demonstrated significantly more knowledge growth than third grade control students. Notably, fourth grade students receiving the intervention demonstrated a similar level of knowledge growth as the fourth-grade control students. Discussion, limitations, and implications related to these findings are addressed. (Contains 4 tables.)

The positive psychology movement seeks to understand student strengths that may facilitate success or promote resilience. However, a positive psychology view is not intended to completely ignore negative factors that may be affecting children, so traditional assessments of mental illness remain important. As methods of assessment improve and enhance our understanding of student development, it is crucial to understand the interrelationship among strength-based and traditional--problem-based--measures. Utilizing a sample of third- and fourth-grade students, the current study examined the interrelationships between three measures of positive functioning, examining hope, life satisfaction, and school connectedness, and their relationship with a traditional, problem-based measure. Results demonstrated that the measures assessing positive constructs were significantly positively correlated with each other and negatively correlated with a measure of problem behaviors. Future directions for research, limitations, and implications for practice are discussed. (Contains 5 tables.)

Universal screening is one strategy to enhance the early identification of behavioral and emotional problems among youth. Although it appears to be effective, it is unclear if universal screening is more or less effective than current teacher referral practices. Thus, the purpose of this study was to compare the effectiveness of a teacher-rated, universal screener and typical teacher-referral methods in identifying youth at risk for emotional and behavioral problems. Results indicated that of the 24 students identified as at-risk by the universal screening measure, only 11 were previously identified through current teacher referral practices--highlighting the potential benefit of universal screening to enhance early identification. Furthermore, results indicated that academic achievement and student engagement outcomes were significantly correlated with at-risk status by identification method. The strengths and limitations of this study, as well as implications for practice, are discussed. (Contains 2 tables.)

Water years (WY) 2017 and 2023 were anomalously wet for California, each alleviating multiyear drought. In both cases, this was unexpected given La Niña conditions, with most seasonal forecasts favoring drier‐than‐normal winters. We analyze over seven decades of precipitation and snow records along with mid‐tropospheric circulation to identify recurring weather patterns driving California precipitation and Sierra Nevada snowpack. Tropical forcing by ENSO causes subtle but important differences in these wet weather patterns, which largely drives the canonical seasonal ENSO‐precipitation relationship. However, the seasonal frequency of these weather patterns is not strongly modulated by ENSO and remains a primary source of uncertainty for seasonal forecasting. Seasonal frequency of ENSO‐independent weather patterns was a major cause of anomalous precipitation in WY2017, record‐setting snow in WY2023, and differences in precipitation outcome during recent El Niño winters 1983, 1998, and 2016. Improved understanding of recurrent atmospheric weather patterns could help to improve seasonal precipitation forecasts. Plain Language Summary: In 2017 and 2023, California experienced unexpectedly wet conditions despite predictions of dry winters due to La Niña. In 2016, seasonal predictions in California favored wet conditions due to the very strong El Niño, but the season was normal‐to‐dry statewide. Understanding relationships between El Niño/La Niña and recurring atmospheric weather patterns driving individual storms is needed to improve seasonal forecasts. We studied historical relationships between weather patterns that bring rain and snow to the region and the El Niño Southern Oscillation (ENSO). We find ENSO influences important characteristics of weather patterns once they make landfall in California, making El Niño storms generally wetter in coastal southern California and Desert Southwest. However, ENSO does not strongly affect how often these patterns occur in a season, which makes seasonal precipitation forecasts challenging. The frequency of certain weather patterns not tied to ENSO played important roles in the unusual rainfall of 2017, the heavy snowfall of 2023, and the drier than expected winter of 2016. Understanding these weather patterns provides operationally and scientifically relevant context for future seasonal forecasts by highlighting that while ENSO only minimally influences the frequency of certain impactful storm types, it does change the precipitation characteristics of these storms. Key Points: Weather regime type and frequency are key drivers of winter seasons with anomalous precipitation and/or snow accumulation in CaliforniaENSO does not modulate the seasonal frequency of weather regimes impacting the coast, presenting a challenge for seasonal forecastingENSO modulates synoptic circulation characteristics of key weather regimes which produces the canonical ENSO‐precipitation relationship [ABSTRACT FROM AUTHOR]

Recent studies indicate poor understanding of the causes and consequences of climate change among college students. In an effort to improve climate change literacy, we have developed an authentic research experience for upper level undergraduate students focused on resolving spatial and temporal patterns of coral reef bleaching, an ecologically and economically important consequence of climate warming. In the research, students use a public archive of maps generated by the United States National Oceanographic and Atmospheric Association (NOAA) that use coloration to depict ocean areas experiencing above-average surface temperatures and where corals are at an increased risk of bleaching. Students are required to quantify the total area of coloration on individual maps using open-source image analysis software called Image J. By quantifying coloration (ie bleaching risk) over a large number of maps in a chronological sequence, students can test hypotheses regarding the relationship between ongoing climate warming and coral bleaching risk. Students are required to summarise their findings in a scientific journal-style report that incorporates graphical representations and statistical tests of their coral bleaching risk data. The research activity is cost-effective, repeatable, requires little specialised knowledge and addresses common programmatic learning outcomes that target scientific communication, quantitative reasoning and sustainability.

This paper considers the impact of global and national political polarisation on perspectives of instructors and learners in Arabic classrooms at Defense Language Institute Foreign Language Center (DLIFLC) in the United States with particular interest in understanding cultural conflicts that occur between instructors and learners. Instructors and learners at DLIFLC responded to a survey, the qualitative data from which are analysed using discourse analysis, research in intercultural communication, and theories of pedagogy. The analysis indicates that while cultural conflict is part of the language-learning experience, there are communicative strategies to turn moments of discord into assets which often involve the ancillary role of non-native speaking instructors who assist in preventing and mediating conflict between native-speaking instructors and students. The paper begins with a brief literature review that informs our theoretical framework, and a description of the study, followed by an analysis of the data it generated and discussion of their implications for DLIFLC and similarly-situated classrooms.

Drained agricultural peatlands occupy only 1% of agricultural land but are estimated to be responsible for approximately one third of global cropland greenhouse gas emissions. However, recent studies show that greenhouse gases fluxes from agricultural peatlands can vary by orders of magnitude over time. The relationship between these hot moments (individual fluxes with disproportionate impact on annual budgets) of greenhouse gas emissions and individual chamber locations (i.e. hot spots with disproportionate observations of hot moments) is poorly understood, but may help elucidate patterns and drivers of high greenhouse gas emissions from agricultural peatland soils. We used continuous chamber-based flux measurements across three land uses (corn, alfalfa, and pasture) to quantify the spatiotemporal patterns of soil greenhouse gas emissions from temperate agricultural peatlands in the Sacramento-San Joaquin Delta of California. We found that the location of hot spots of emissions varied over time and were not consistent across annual timescales. Hot moments of nitrous oxide (N2O) and carbon dioxide (CO2) fluxes were more evenly distributed across space than methane (CH4). In the corn system, hot moments of CH4 flux were often isolated to a single location but locations were not consistent across years. Spatiotemporal variability in soil moisture, soil oxygen, and temperature helped explain patterns in N2O fluxes in the annual corn agroecosystem but were less informative for perennial alfalfa N2O fluxes or CH4 fluxes across ecosystems, potentially due to insufficient spatiotemporal resolution of the associated drivers. Overall, our results do not support the concept of persistent hot spots of soil CO2, CH4, and N2O emissions in these drained agricultural peatlands. Hot moments of high flux events generally varied in space and time and thus required high sample densities. Our results highlight the importance of constraining hot moments and their controls to better quantify ecosystem greenhouse gas budgets. [ABSTRACT FROM AUTHOR]

Morro manzanita Arctostaphyos morroensis (Ericaceae) is a long-lived, shrub endemic to San Luis Obispo County, southern California, USA. It was listed as threatened under the U.S. Endangered Species Act in 1994, with identified threats being residential and urban development, including lack of protection on private land and lack of management on public lands, competition with invasive non-native plants, and risks of extinction associated with small and isolated populations. Our goal in this paper is to summarize and supplement the current knowledge of Morro manzanita. We review the literature on the species' description, reproductive ecology, germination cues, short-term response to fire. and distribution. We conducted field surveys to report on long-term response to fire, resampling the previously studied prescribed burn site 25 yr post-fire. Finally, we summarize the current land management of sites that support Morro manzanita and threats faced by this species. We conclude with specific recommendations for management and future study towards supporting conservation of this species and its maritime chaparral community. [ABSTRACT FROM AUTHOR]

Background: A California-based health plan offered home-based palliative care (HBPC) to members who needed support at home but did not yet qualify for hospice. Objectives: This study compares hospital and emergency department (ED) utilization and costs and mortality for individuals receiving HBPC to a cohort not receiving palliative care services (Usual Care). Design: This is an observational retrospective study using claims data covering a prestudy period and a study period during which time half of the study population received HBPC services. Setting/Subjects: Seriously ill individuals who received HBPC were matched with those receiving Usual Care using a propensity-based matching algorithm. Intervention: Interdisciplinary teams from home health and hospice agencies provided HBPC services. Measurements: Outcome measures included hospital and ED utilization and cost before and during the study period and mortality during the study period. Results: For both groups, hospital and ED utilization and associated costs were higher during the prestudy period than during the study period. No differences were found in outcome measures between groups during the study period. Average time in the study period was longer for the HBPC group than that in the Usual Care group, indicating that they lived longer or transitioned to hospice later. Conclusion: Although individuals in both groups were living with serious illnesses for which worsening health and increased acute care utilization are expected over time, both groups had reduced acute care utilization and costs during the study period compared with the prestudy period. Reduced utilization and costs were equivalent for both groups. [ABSTRACT FROM AUTHOR]

Decarceration policies, enacted for SARS-CoV-2 mitigation in carceral settings, potentially exacerbated barriers to care for people living with HIV (PWH) with criminal legal involvement (CLI) during Shelter-in-Place (SIP) by limiting opportunities for engagement in provisions of HIV and behavioral health care. We compared health care engagement for PWH with CLI in San Francisco, California before and after decarceration and SIP using interrupted time series analyses. Administrative data identified PWH booked at the San Francisco County Jail with at least one clinic encounter from 01/01/2018-03/31/2020 within the municipal health care network. Monthly proportions of HIV, substance use, psychiatric and acute care encounters before (05/01/2019-02/29/2020) and after (03/01/2020-12/31/2020) SIP and decarceration were compared using Generalized Estimating Equation (GEE) log-binomial and logistic regression models, clustering on the patient-level. Of 436 patients, mean age was 43 years (standard-deviation 11); 88% cisgender-male; 39% white, 66% homeless; 67% had trimorbidity by Elixhauser score (medical comorbidity, psychotic disorder or depression, and substance use disorder). Clinical encounters immediately dropped following SIP for HIV (aOR = 0.77; 95% CI: 0.67, 0.90) and substance use visits (aRR = 0.83; 95% CI: 0.70, 0.99) and declined in subsequent months. Differential reductions in clinical encounters were seen among Black/African Americans (aRR = 0.93; 95% CI: 0.88, 0.99) and people experiencing homelessness (aRR = 0.92; 95% CI: 0.87, 0.98). Significant reductions in care were observed for PWH with CLI during the COVID-19 pandemic, particularly among Black/African Americans and people experiencing homelessness. Strategies to End the HIV Epidemic must improve engagement across diverse care settings to improve outcomes for this key population. [ABSTRACT FROM AUTHOR]

School climate has been lauded for its relationship to a host of desirable academic, behavioral, and social-emotional outcomes for youth. The present study tested the hypothesis that school climate counteracts youths' home-school risk by examining the moderating effects of students' school climate perceptions on the relationship between family structure (i.e., two-parent, one-parent, foster-care, and homeless households), and academic performance (i.e., self-reported [grade point average] GPA). The present sample consisted of 902 California public high schools, including responses from over 490,000 students in Grades 9 and 11. Results indicated that, regardless of family structure, students with more positive school climate perceptions self-reported higher GPAs. Youths with two-parent, one-parent, and homeless family structures displayed stepwise, linear improvements in self-reported GPA as perceptions of climate improved. Foster-care students' positive school climate perceptions had a weaker effect on their self-reported GPA compared with students living in other family structures. A unique curvilinear trend was found for homeless students, as the relationship between their school climate perceptions and self-reported GPA was stronger at lower levels. Overall, the moderation effect of positive school climate perceptions on self-reported GPA was strongest for homeless youth and youth from one-parent homes, suggesting that school climate has a protective effect for students living in these family structures. A protective effect was not found for youth in foster-care. Implications for research and practice are discussed.

The fish communities of the Sacramento-San Joaquin Delta and its tributaries in California's Central Valley have been irreparably altered through introductions of numerous fish species, including Striped Bass (Morone saxatilis), black bass (Micropterus spp.), and catfishes (Ameiurus spp. and Ictalurus spp.). Research into how predation by non-native piscivores affects native anadromous species has focused on the Sacramento and San Joaquin river mainstems and Delta habitats, through which all anadromous species must pass. Yet, the ranges of nonnative fishes extend into upstream tributaries. We collected diets from native and non-native piscivores in the Stanislaus River, a tributary to the San Joaquin River and a remaining stronghold for native fishes. Piscivorous fishes primarily consumed invertebrates and the native species fall-run Chinook Salmon (Oncorhynchus tshawytscha) and Pacific Lamprey (Entosphenus tridentatus). Juvenile Chinook Salmon and Pacific Lamprey were consumed at higher frequencies than any other potential fish prey species, particularly by Striped Bass and black bass. The frequency of native fishes in predator diets was similar across years, despite contrasting hydrologic conditions; 2019 (wet year), 2020 (dry year), and 2021 (critically dry year). Our results show that Pacific Lamprey were frequently consumed by native and non-native piscivores, and that juvenile Chinook Salmon experience substantial predation early in their migration, regardless of hydrologic conditions. [ABSTRACT FROM AUTHOR]

Sea‐level rise is a "nascent subsystem" with policy actors actively developing initial policy preferences through participation across multiple policy forums in a polycentric system. This paper uses item‐response models to empirically analyze how actors, perceived problems, and preferred policy solutions are related in a latent "policy space". We focus on California's San Francisco Bay region, where in the last decade, sea‐level rise emerged to become one of the most salient climate adaptation issues. We find that actors and policy preferences are arranged in a two‐dimensional space, with highly engaged actors more likely to consider environmental justice and ecological aspects of sea level rise. Our findings have implications for theories of nascent subsystems within the Advocacy Coalition Framework, and for understanding how a local policy subsystem develops to address and prioritize the multi‐faceted impacts of climate change. [ABSTRACT FROM AUTHOR]

Local governments consider a wide range of policies to increase resilience in the face of myriad risks and employ a variety of tactics to communicate about these policies to external actors. An important platform to signal resilience as a policy priority is through the budget process wherein local communities decide "who gets what, when, and how." Using computational text mining techniques, we assess how county governments in California signal efforts toward resilience in their budgets during the 2012–2017 fiscal years, as well as whether and how those signals are received by the capital market. Comparable budget documents are available for 38 counties across the state for a total of 161 county-year observations. To test the relationship between local government resilience signals and capital market outcomes, we focus on county underlying credit ratings issued by counties. Empirical results show that county underlying credit ratings are insensitive to resilience signals in local government budgets. By examining the efficacy of resilience signals and their effects on the capital market, we offer evidence on the link between policy signaling and financial outcomes at the local government level. [ABSTRACT FROM AUTHOR]

This article presents the story of how Pepperdine University migrated its library management functions to the cloud using what is now known as OCLC's WorldShare Management Services (WMS). The story of implementing this new service is told from two vantage points: (1) that of the library; and (2) that of the service provider. The authors were the principle collaborators for this pilot project, which took place between June and December 2010. In this article, the authors outline the stages of adopting and deploying WMS. The process described and the advice shared, however, apply to the adoption of other systems as well.

Public speaking will continue to be an unsettling experience for some students, including those with disabilities. Experts have suggested several reasons for fearing public speaking; adequate preparation and practice can alleviate most of them. Using the PAVES (Posture, Attitude, Voice, Eye Contact, Smile) strategy described in this article can provide teachers and students with a systematic plan when preparing for public speaking and presentations. The PAVES strategy encompasses components that prompt students' internal and external behaviors toward successful public speaking and presentations. PAVES may be especially helpful for students with disabilities as they are included in general education curriculum and in intervention programs. (Contains 2 figures.)

Environmental impact assessment (EIA) is a decision support tool that analyzes the environmental and social impacts of infrastructure projects. This paper focuses on the California Environmental Quality Act (CEQA), a law requiring EIA use in California, to examine where new infrastructure is proposed and whether EIA can shape infrastructure distribution and environmental justice through the review process. We analyze the temporal and spatial distribution of more than 7000 infrastructure projects and their environmental impacts as proposed under CEQA from 2011 to 2020. Using fixed-effects negative binomial regression to model the association between the number of initiated projects and existing socioeconomic and environmental conditions by census tract, and multinomial logistic regression to investigate determinants of a project's level of environmental review, we find an unequal distribution of infrastructure. We find that socio-economically vulnerable communities and those with greater burden of environmental pollution are less likely to be the site of newly proposed infrastructure, but that proposed projects tend to be beneficial, less-polluting infrastructure like parks or schools that could help redress past injustices. Moreover, projects proposed in vulnerable communities are less likely to receive the most stringent reviews or have their impacts mitigated. These findings suggest that CEQA interacts with distributive justice in contradictory ways. They also highlight the need to separately consider environmental amenities versus harms such that EIA processes do not stand as a barrier to constructing beneficial infrastructure in environmental justice communities. • New and upgraded infrastructure helps overcome historic distributional injustices burdening poor, minority communities. • In California, new infrastructure is proposed less often in socioeconomically vulnerable and more polluted neighborhoods. • California's environmental impact assessment (EIA) law is inconsistently addressing distributional justice. • Projects proposed in less vulnerable communities likely receive more stringent EIA reviews and have their impacts mitigated. • EIA is not designed to consider environmental amenities and may limit beneficial infrastructure from redressing past harms. [ABSTRACT FROM AUTHOR]

Background: Kratom (Mitragyna speciosa) use in the United States is becoming increasingly popular and its legal status varies widely from state to state. Multiple reports of adverse events associated with kratom use have ranged from liver injury, seizures, psychiatric disturbance, and rarely death. Methods: This study investigated coroner autopsy reports from Kern County in California for the year 2020 which included qualitative data on substances from blood toxicological reports. Of the 214 opioid-associated accidental overdoses reported, 4 subjects (1.9%) had mitragynine (kratom) exposure on the autopsy report and were included in the study. We reported available demographic information and comorbid substance findings from the associated autopsy reports. Results: All 4 individuals with mitragynine (kratom) toxicology had accidental opioid overdose deaths noted in autopsy reports. Each subject also had toxicology positive for at least one other substance. Fentanyl was found in 3 (75%) of the cases and suspected to be the primary contributor to opioidrelated deaths in those cases. However, one fatality was without fentanyl, but instead had tested positive for benzodiazepines, cannabis, and other psychiatric medications. Discussion: The findings of this brief report provide insight into the role that mitragynine (kratom) may have in modulating risk of opioid-related deaths. The combined use of kratom with opioids such as fentanyl appears most likely to increase the risk of a fatal overdose, but it may also occur with other medications such as benzodiazepines and psychiatric medications. It is a serious concern that in the midst of the opioid overdose epidemic there is a growing presence of kratom use in the U.S. population with a largely unregulated status. [ABSTRACT FROM AUTHOR]

Background: Antimicrobial resistant infections continue to be a leading global public health crisis. Mobile genetic elements, such as plasmids, have been shown to play a major role in the dissemination of antimicrobial resistance (AMR) genes. Despite its ongoing threat to human health, surveillance of AMR in the United States is often limited to phenotypic resistance. Genomic analyses are important to better understand the underlying resistance mechanisms, assess risk, and implement appropriate prevention strategies. This study aimed to investigate the extent of plasmid mediated antimicrobial resistance that can be inferred from short read sequences of carbapenem resistant E. coli (CR-Ec) in Alameda County, California. E. coli isolates from healthcare locations in Alameda County were sequenced using an Illumina MiSeq and assembled with Unicycler. Genomes were categorized according to predefined multilocus sequence typing (MLST) and core genome multilocus sequence typing (cgMLST) schemes. Resistance genes were identified and corresponding contigs were predicted to be plasmid-borne or chromosome-borne using two bioinformatic tools (MOB-suite and mlplasmids). Results: Among 82 of CR-Ec identified between 2017 and 2019, twenty-five sequence types (STs) were detected. ST131 was the most prominent (n = 17) followed closely by ST405 (n = 12). blaCTX−M were the most common ESBL genes and just over half (18/30) of these genes were predicted to be plasmid-borne by both MOB-suite and mlplasmids. Three genetically related groups of E. coli isolates were identified with cgMLST. One of the groups contained an isolate with a chromosome-borne blaCTX−M−15 gene and an isolate with a plasmid-borne blaCTX−M−15 gene. Conclusions: This study provides insights into the dominant clonal groups driving carbapenem resistant E. coli infections in Alameda County, CA, USA clinical sites and highlights the relevance of whole-genome sequencing in routine local genomic surveillance. The finding of multi-drug resistant plasmids harboring high-risk resistance genes is of concern as it indicates a risk of dissemination to previously susceptible clonal groups, potentially complicating clinical and public health intervention. [ABSTRACT FROM AUTHOR]

Alfalfa is the most widely grown forage crop worldwide and is thought to be a significant carbon sink due to high productivity, extensive root systems, and nitrogen-fixation. However, these conditions may increase nitrous oxide (N2O) emissions thus lowering the climate change mitigation potential. We used a suite of long-term automated instrumentation and satellite imagery to quantify patterns and drivers of greenhouse gas fluxes in a continuous alfalfa agroecosystem in California. We show that this continuous alfalfa system was a large N2O source (624 ± 28 mg N2O m2 y−1), offsetting the ecosystem carbon (carbon dioxide (CO2) and methane (CH4)) sink by up to 14% annually. Short-term N2O emissions events (i.e., hot moments) accounted for ≤1% of measurements but up to 57% of annual emissions. Seasonal and daily trends in rainfall and irrigation were the primary drivers of hot moments of N2O emissions. Significant coherence between satellite-derived photosynthetic activity and N2O fluxes suggested plant activity was an important driver of background emissions. Combined data show annual N2O emissions can significantly lower the carbon-sink potential of continuous alfalfa agriculture. Long-term continuous greenhouse gas measurements in alfalfa cropland showed that the magnitude of the carbon sink was significantly offset by large nitrous oxide (N2O) emission events following irrigation and rainfall. [ABSTRACT FROM AUTHOR]

Increasing wildfire and declining snowpacks in mountain regions threaten water availability. We combine satellite‐based fire detections with snow seasonality classifications to examine fire activity in California's seasonal and ephemeral snow zones. We find a nearly tenfold increase in fire activity during 2020–2021 versus 2001–2019. Accumulation season broadband snow albedo declined 25%–71% at two burned sites (2021 and 2022) according to in‐situ data relative to un‐burned conditions, with greater declines associated with increased burn severity. By enhancing snowpack susceptibility to melt, both decreased snow albedo and canopy drove midwinter melt during a multi‐week dry spell in 2022. Despite similar meteorological conditions in December–February 2013 and 2022–linked to persistent high pressure weather regimes–minimal melt occurred in 2013. Post‐fire snowpack differences are confirmed with satellite measurements. With growing geographical overlap between wildfire and snow, our findings suggest California's snowpack is increasingly vulnerable to the compounding effects of dry spells and wildfire. Plain Language Summary: Satellite fire detections indicate substantial increases in wildfire activity in California's snow‐covered landscapes during 2020 and 2021, suggesting wildfire is increasingly altering mountain hydrology. During 2022, a multi‐week mid‐winter drought, or dry spell, occurred. A meteorologically‐similar dry spell occurred in 2013, and the 2022 event provides a test case to examine how post‐fire changes (canopy loss and deposition of burned dark material on snowpack) alter snowmelt patterns. Using field observations, weather station data, and satellite remote sensing of snow, we find large reductions in snow albedo and canopy cover drove rapid melt during the 2022 dry spell in burned areas whereas during 2013, minimal melt occurred. The societal connection between mountains and humans will be strained as mountains face increasing climate‐related stressors. Midwinter drought, snow loss, and increasing wildfire are expectations of a warming world. Addressing these challenges requires innovative water and forest management paradigms. Our findings motivate additional research into assessing and planning for post‐fire hydrologic changes in snow‐dominated landscapes as both wildfire and dry spells will increase in frequency with climate warming. Key Points: A 9.8x increase in satellite fire detections in California's snow zones in 2020–2021 versus 2001–2019 implies growing overlap in fire and snowPost‐fire accumulation season broadband snow albedo declined 25%–71%, driving fewer snow‐covered days and lower snow‐cover fractionCompared with the meteorologically similar 2013 dry spell, albedo and canopy declines led to rapid midwinter melt in 2022 [ABSTRACT FROM AUTHOR]

California is the leading dairy state in the United States. The total sale of milk and its products represents about $6.3 billion annually out of the $50 billion generated from all agricultural production in the state. However, methane emissions from dairy manure and enteric fermentation represented nearly half of all annual methane emissions in California, with dairy manure accounting for 25%, and enteric fermentation for 20%. Methane emissions originating from manure are produced primarily from anaerobic settling basins and lagoons, which are the most common manure storage systems in the state. To achieve sustainability on dairy farms and to comply with state regulations for air and climate pollutants, dairy farms have implemented technologies such as anaerobic digestion and alternative manure management technologies. In addition, governmental incentive programs have been deployed to partially fund these technologies for eligible dairies in the state. The present article reviews the design and operations, effectiveness, and economics of the most common technologies employed in Californian dairies in reducing methane emissions. The technologies studied include anaerobic digesters, mechanical separators, compost-bedded pack barns, manure vacuuming followed by drying, and weeping walls. The current status and estimated effectiveness of government incentive programs are reviewed and recommendations for improvements presented. Finally, future trends and research needs for mitigating the emissions in Californian dairies are identified. [ABSTRACT FROM AUTHOR]