1. Bioactive secondary metabolites from new endophytic fungus Curvularia. sp isolated from Rauwolfia macrophylla.
- Author
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Kaaniche, Fatma, Hamed, Abdelaaty, Abdel-Razek, Ahmed S., Wibberg, Daniel, Abdissa, Negera, El Euch, Imene Zendah, Allouche, Noureddine, Mellouli, Lotfi, Shaaban, Mohamed, and Sewald, Nobert
- Subjects
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METABOLITES , *ENDOPHYTIC fungi , *RIBOSOMAL DNA , *MASS spectrometry , *HOST plants , *NUCLEAR magnetic resonance spectroscopy , *CURVULARIA - Abstract
Over the last decades, endophytic fungi represent a new source of pharmacologically active secondary metabolites based on the underlying assumption that they live symbiotically within their plant host. In the present study, a new endophytic fungus was isolated from Rauwolfia macrophylla, a medicinal plant from Cameroon. The fungus showed a highest homology to Curvularia sp. based on complete nucleotide sequence data generated from the internal transcribed spacer (ITS) of ribosomal DNA region. Large scale fermentation, working-up and separation of the strain extract using different chromatographic techniques afforded three bioactive compounds: 2'-deoxyribolactone (1), hexylitaconic acid (2) and ergosterol (3). The chemical structures of compounds 1–3 were confirmed by 1 and 2D NMR spectroscopy and mass spectrometry, and comparison with corresponding literature data. Biologically, the antimicrobial, antioxidant activities and the acetylcholinesterase inhibitory of the isolated compounds were studied. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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