Search

Your search keyword '"Hoff NA"' showing total 2 results
Start Over Author "Hoff NA" Region cameroon
2 results on '"Hoff NA"'

1. Seroreactivity against Marburg or related filoviruses in West and Central Africa.

Author

Steffen I, Lu K, Hoff NA, Mulembakani P, Okitolonda Wemakoy E, Muyembe-Tamfum JJ, Ndembi N, Brennan CA, Hackett J Jr, Switzer WM, Saragosti S, Mbensa GO, Laperche S, Rimoin AW, and Simmons G

Subjects
Animals, Cameroon epidemiology, Enzyme-Linked Immunosorbent Assay, Filoviridae genetics, Filoviridae Infections blood, Filoviridae Infections epidemiology, Filoviridae Infections virology, Ghana epidemiology, Humans, Marburg Virus Disease virology, Marburgvirus genetics, Retrospective Studies, Seroepidemiologic Studies, Antibodies, Viral blood, Filoviridae immunology, Marburg Virus Disease blood, Marburg Virus Disease epidemiology, Marburgvirus immunology
Abstract

A serological survey of 2,430 archived serum samples collected between 1997 and 2012 was conducted to retrospectively determine the prevalence of Marburg virus in five African countries. Serum samples were screened for neutralizing antibodies in a pseudotype micro-neutralization assay and confirmed by enzyme-linked immunosorbent assay (ELISA). Surprisingly, a seroprevalence for Marburg virus of 7.5 and 6.3% was found in Cameroon and Ghana, respectively, suggesting the circulation of filoviruses or related viruses outside of known endemic areas that remain undetected by current surveillance efforts. However, due to the lack of validated assays and appropriate positive controls, these results must be considered preliminary.

Published
2020
Full Text
View/download PDF

2. High Herpesvirus Diversity in Wild Rodent and Shrew Species in Central Africa.

Author

Ntumvi NF, Mbala Kingebeni P, Tamoufe U, Kumakamba C, Ndze V, Ngay Lukusa I, LeBreton M, Atibu Losoma J, Le Doux Diffo J, N'Kawa F, Takuo JM, Mulembakani P, Nwobegahay J, Makuwa M, Muyembe Tamfum JJ, Gillis A, Harris S, Rimoin AW, Hoff NA, Fair JM, Monagin C, Ayukekbong J, Rubin EM, Wolfe ND, and Lange CE

Subjects
Animals, Asia, Cameroon, DNA, Viral genetics, Democratic Republic of the Congo, Herpesviridae genetics, Phylogeny, Polymerase Chain Reaction, Sequence Analysis, DNA, DNA, Viral analysis, Genetic Variation, Herpesviridae classification, Herpesviridae isolation & purification, Rodentia virology, Shrews virology
Abstract

Objective: Herpesviruses belong to a diverse order of large DNA viruses that can cause diseases in humans and animals. With the goal of gathering information about the distribution and diversity of herpesviruses in wild rodent and shrew species in central Africa, animals in Cameroon and the Democratic Republic of the Congo were sampled and tested by PCR for the presence of herpesvirus DNA., Methods: A broad range PCRs targeting either the Polymerase or the terminase gene were used for virus detection. Amplified products from PCR were sequenced and isolates analysed for phylogenetic placement., Results: Overall, samples of 1,004 animals of various rodent and shrew species were tested and 24 were found to be positive for herpesvirus DNA. Six of these samples contained strains of known viruses, while the other positive samples revealed DNA sequences putatively belonging to 11 previously undescribed herpesviruses. The new isolates are beta- and gammaherpesviruses and the shrew isolates appear to form a separate cluster within the Betaherpesvirinae subfamily., Conclusion: The diversity of viruses detected is higher than in similar studies in Europe and Asia. The high diversity of rodent and shrew species occurring in central Africa may be the reason for a higher diversity in herpesviruses in this area., (© 2018 S. Karger AG, Basel.)

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results