1. Frequency of HLA-A, -B and -DRB1 specificities and haplotypic associations in the population of Castilla y León (northwest-central Spain).
- Author
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Alcoceba, M., Marín, L., Balanzategui, A., Sarasquete, M. E., Chillón, M. C., Martín-Jiménez, P., Puig, N., Santamaría, C., Corral, R., García-Sanz, R., San Miguel, J. F., and González, M.
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GENE frequency , *HLA histocompatibility antigens , *OLIGONUCLEOTIDES , *POLYMERASE chain reaction , *NUCLEOTIDE sequence , *MEDICAL genetics - Abstract
The frequencies of human leukocyte antigen (HLA) class I and class II specificities and haplotypic associations were determined in 1940 unrelated donors from Castilla y León and compared with other Iberian, Mediterranean and European populations. Specificities were determined using polymerase chain reaction reverse sequence-specific oligonucleotide or polymerase chain reaction sequence-specific primer techniques. In the analysis, 19, 29 and 13 specificities were found for HLA-A, -B and -DRB1, respectively, with HLA-A*02 (26%), - A*01 (11%), - B*44 (16%), - B*35 (10%), - DRB1*07 (16%) and - DRB1*13 (14%) showing the highest frequencies. In addition, 10 common HLA-A-B-DRB1 haplotypic associations were observed, A*01-B*08-DRB1*03 (3%) and A*29-B*44-DRB1*07 (3%) being the most frequent ones. These findings indicate that the population of Castilla y León is genetically equidistant from the Portuguese and other Spanish populations and shares a common origin with other Iberian populations, in which European, Mediterranean and North African genetic components are present; this is in agreement with the historical and genetic background of the population. These data contribute to a better understanding of the genetic structure of the Iberian Peninsula and provide a healthy control population from our region that should be useful for the study of disease associations. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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