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Research conducted since 2003 in and around the regional center of Tres Zapotes, Veracruz documents the importance of aquatic resources and transportation routes for subsistence and settlement over more than two millennia. Systematic archaeological survey over more than 400 km2 in the surrounding Eastern Lower Papaloapan Basin details a persistent focus of major settlements on streams and other bodies of water that would have provided an abundance of food and opportunities for water-born transport. Excavations at Tres Zapotes further indicate that exploitation of aquatic fauna was a significant component of a mixed subsistence strategy for the Olmec and Epi-Olmec inhabitants of the site, who obtained fish, turtles, and waterfowl from streams, lakes, estuaries and coastal marine environments over a range extending no less than 20 km from the center. Artifact inventories and landscape features further suggest an associated subsistence technology that included weighted nets and retention ponds in seasonally flooded areas. [ABSTRACT FROM AUTHOR]