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Prangos belongs to the Apiaceae family. It's wildly distributed from the Mediterranean region to the Western and Central Asia. P. pabularia is one of the most investigated species among the 72 species and it's indigenous to India [1]. This plant produces a large number of coumarins and has been found to be relatively rich in secondary metabolic products [2]. The n-butanol fraction (100 g) was applied to the silica gel column (100-200 mesh), and eluted with a gradient solvent of dichloromethane: methanol by increasing the polarity. Five individual compounds were obtained by further isolation and purification of the obtained fractions by different chromatography techniques and semi-preparative HPLC. The structures of individual compounds were established according to the spectroscopic data ID, 2D NMR, HR-MS and comparison of the obtained data with the reported data. Isolated compounds from this fraction were identified as yuganin B (1), 1'-O-β-D-glucopyranosyl-(2'S)-marmesin (2) [3], oxypeucedanin hydrate 3'-O-β-D-glucopyranoside (3), duharin B (4) [4] and 1'-O-β-Dglucopyranosyl-(2'S,3'R)-3'-hydroxymarmesin (5). Compound 1 was a new coumarin, while compounds 2, 4 and 5 were isolated from this species for the first time. [ABSTRACT FROM AUTHOR]

Diterpenoid alkaloids are a kind of nitrogen-containing organic compounds with wide range of biological activities and medicinal value, especially in the aspects of antiarrhythmic and analgesia. Four diterpenoid alkaloids have been used in clinical. At present, more than one thousand natural diterpenoid alkaloids have been found, which are mainly from genera Delphinium and Aconitum of Ranunculaceae family. It was found that the diterpenoid alkaloids from the characteristic Delphinium and Aconitum plants in Central Asia have the characteristics of diverse skeleton types, high novelty and great potential for medicine, which have a great value for in-depth research and development. On the basis of the existing research, Aconitum barbatum var. puberulum and Delphinium aemulans grown in Central Asia were chosen as the research objects. The isolation, purification, structure identification and bioactivity screening of diterpenoid alkaloids were carried out. 60 compounds, including 37 diterpenoid alkaloids were isolated from these two plants. Of which 13 of diterpenoid alkaloids were newcompounds, see Fig. 1. Compound AB-1 is the first discovered new skeleton type of C20-diterpenoid alkaloid, while compounds AB-6 and AB-7 are the first discovered rearranged of C18-diterpenoid alkaloids. The isolated diterpenoid alkaloids involve 3types of skeletons and 9 subtypes of skeletons. The activity screening results showed that some compounds exhibited moderate potential for antiarrhythmia, anti-tumor, and antibacterial activities. [ABSTRACT FROM AUTHOR]