1. ANALYSIS AND RESEARCH ON THE DIVERSITY OF MICROBIAL COMMUNITY STRUCTURE IN URBAN RIVER SEDIMENT.
- Author
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TU, B. H., OU, Y. T., ZHAO, Y., QIN, W., XIAO, X., ZHANG, S., and LIU, Y.
- Subjects
RIVER sediments ,COMMUNITY organization ,MICROBIAL communities ,DENATURING gradient gel electrophoresis ,GENE amplification ,GEL electrophoresis ,MICROBIAL diversity - Abstract
In this study, sediment was sampled from the North River in Changzhou at five different loca-tions at the same time. Microbial DNA was extracted from the obtained samples, and the extracted DNA purity was compared by two different methods. After DNA amplification by 16S rRNA PCR, microspectrophotometry and agarose gel electrophoresis, the modified gradient gel electrophoresis (DGGE) was performed. The DGGE map was analyzed using Quantity one software of bio-rad to study the community diversity. The similarity matrix showed that the samples of different locations and time had high similarity, with the highest similarity reaching 82%. The proportion of similarity over 60% is extremely high, which indicates that the microbial population in North River is abundant, but the microbial community structure changes little in space. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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