1. Assessment of Analytical Reproducibility of 1H NMR Spectroscopy Based Metabonomics for Large-Scale Epidemiological Research: the INTERMAP Study.
- Author
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Dumas, Marc-Emmanuel, Maibaum, Elaine C., Teague, Claire, Ueshima, Hirotsugu, Zhou, Beifan, Lindon, John C., Nicholson, Jeremy K., Stamler, Jeremiah, Elliott, Paul, Chan, Queenie, and Holmes, Elaine
- Subjects
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NUCLEAR magnetic resonance spectroscopy , *BIOMARKERS , *NUCLEAR spectroscopy , *URINALYSIS , *ANALYTICAL chemistry , *BIOCHEMISTRY - Abstract
Large-scale population phenolyping for molecular epidemiological studies is subject to all the usual criteria of analytical chemistry. As part of a major phenotyping investigation we have used high-resolution ¹H NMR spectroscopy to characterize 24-h urine specimens obtained from population samples in Aito Town, Japan (n = 259), Chicago, IL(n = 315), and Guangxi, China (n = 278). We have investigated analytical reproducibility, urine specimen storage procedures, interinstrument variability, and split specimen detection. Our data show that the multivariate analytical reproducibility of the NMR screening platform was >98% and that most classification errors were due to urine specimen handling inhomogeneity. Differences in metabolite profiles were then assessed for Alto Town, Chicago, and Guangxi population samples; novel combinations of biomarkers were detected that separated the population samples. These cross-population differences in urinary metabolites could be related to genetic, dietary, and gut microbial factors. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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