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Lack of student motivation is a detrimental problem in foreign language education. This problem may lead to less target language production and lower proficiency. The purpose of this quasi-experimental study was to examine the effect of change in student motivation when a gamified intervention is implemented. This study was conducted in an English foreign language course at a secondary school in the inner city of Temuco, Chile. Student motivation was measured by using a 22 item, self-reporting questionnaire with a six-point Likert scale. A total of 53 students participated in this study. A quantitative analysis indicated that the experimental group scored higher on the questionnaire in all but one of the items. An independent sample t-test indicated that 19 of the items that scored higher were statistically significant. These results show that the use of a gamified intervention can positively affect student motivation.

Natural habitats in South America have been intensively used and modified, including their conversion to exotic tree plantations, impacting the terrestrial fauna communities. Carnivores play an important role in the functioning of ecosystems as apex predators. Landscape characteristics and resource availability determine the composition and abundance of co-occurring carnivores. We hypothesized that the diversity and relative abundance of native carnivores varied in relation to the different vegetation macrohabitats. We predicted that native forests support a higher diversity and abundance of native carnivores compared to exotic plantations. Between 1 March 2021 and 31 March 2022, we estimated the species' richness, composition, and relative abundance of terrestrial native carnivores in three different landscapes of the Coast Range in central Chile: (a) monoculture plantation of the exotic Pinus radiata (MP); (b) Mediterranean coastal thorn forest (TF); and (c) Mediterranean coastal sclerophyllous forest (SF). We monitored an area of 1000 ha in each landscape, installing 10 camera traps (total of 30 camera traps). We used a monitoring transect with unbaited camera traps separated by 1 km, with a total photo-trapping effort of 10,046 camera days. The classification, organization, and analysis of camera trap data were conducted using CameraSweet software. The total number of independent native carnivore species events (photos separated > 60 min) recorded in each landscape were 1564 in SF, 1412 in TF, and 775 in MP. Carnivore richness and composition were not significantly different in all three landscapes. We detected five native carnivore species. Relative abundance by species was significantly higher in SF compared to MP for Leopardus guigna, Conepatus chinga, and Galictis cuja and also significantly higher in TF compared to MP for L. guigna. Our results suggest that the native vegetation cover must be preserved to maintain viable and abundant native carnivore populations, crucially relevant in the highly impacted and human-dominated global biodiversity hotspot of Central Chile. This study contributes to informing evidence-based decision-making and conservation strategies at the landscape level to mitigate biodiversity loss. [ABSTRACT FROM AUTHOR]

Darwin's fox is an opportunistic omnivorous predator native to Chile classified as endangered by the IUCN Red List. Habitat use by Darwin's foxes can be negatively affected by the presence of free-ranging dogs that range freely across native and non-native habitats and can be a source of fox mortality. The objective of this study was to analyze the isotopic similarity of Darwin's fox and sympatric free-ranging dogs in Chiloé Island to determine the impact of anthropogenic environmental alterations on wild predators. We use hair samples to characterise and compare their δ13C and δ15N values and to evaluate isotopic similarity and isotope niches overlap. A generalised linear model was used to associate the isotope value with landscape variables (forest cover and vegetation type) and distance to the nearest house. We found no significant differences in δ13C or δ15N values between foxes and dogs, and a marginally significant isotope niche overlap (59.4 %). None of the selected variables at landscape and site scale were related to isotope values. Although our study is not a probe of direct contact between foxes and free-ranging dogs, the high isotopic similarity highlights the risk of pathogen spillover from free-ranging dogs to Darwin's foxes. [ABSTRACT FROM AUTHOR]

Landscape anthropization has been identified as one of the main drivers of pathogen emergence worldwide, facilitating pathogen spillover between domestic species and wildlife. The present study investigated Carnivore protoparvovirus‐1 infection using molecular methods in 98 free‐ranging wild guignas (Leopardus guigna) and 262 co‐occurring owned, free‐roaming rural domestic cats. We also assessed landscape anthropization variables as potential drivers of infection. Protoparvovirus DNA was detected in guignas across their entire distribution range, with observed prevalence of 13.3% (real‐time PCR) and 9% (conventional PCR) in guignas, and 6.1% (conventional PCR) in cats. Prevalence in guigna did not vary depending on age, sex, study area or landscape variables. Prevalence was higher in juvenile cats (16.7%) than in adults (4.4%). Molecular characterization of the virus by amplification and sequencing of almost the entire vp2 gene (1,746 bp) from one guigna and five domestic cats was achieved, showing genetic similarities to canine parvovirus 2c (CPV‐2c) (one guigna and one cat), feline panleukopenia virus (FPV) (one cat), CPV‐2 (no subtype identified) (two cats), CPV‐2a (one cat). The CVP‐2c‐like sequence found in a guigna clustered together with domestic cat and dog CPV‐2c sequences from South America, suggesting possible spillover from a domestic to a wild species as the origin of infection in guigna. No clinical signs of disease were found in PCR‐positive animals except for a CPV‐2c‐infected guigna, which had haemorrhagic diarrhoea and died a few days after arrival at a wildlife rescue centre. Our findings reveal widespread presence of Carnivore protoparvovirus‐1 across the guigna distribution in Chile and suggest that virus transmission potentially occurs from domestic to wild carnivores, causing severe disease and death in susceptible wild guignas. [ABSTRACT FROM AUTHOR]

Carnivore protoparvovirus 1 is one of the most important pathogens affecting both wild and domestic carnivores. Here, we reported the genetic characterization of canine parvovirus (CPV‐2) strains from a rescued guiña (Leopardus guigna) and domestic dogs from Chile. Guiña strain was classified as CPV‐2c, and phylogenetic analysis of the complete coding genome showed that the guiña CPV‐2c strain shares a recent common ancestor with Chilean domestic dogs' strains. These viruses showed >99% identity and exhibited three changes in the NS1 protein (V596A, E661K and L582F). This is the first detection and genetic characterization of CPV‐2c infection in guiña worldwide, and one of the few comparative studies that show the source of infection was domestic dogs. The current findings highlight the fact that guiña is a susceptible species to protoparvovirus infection and that domestic dogs represent an important threat to its conservation. The CPV‐2 cross‐species transmission between domestic dogs and guiña should be taken into account for protection programmes of this endangered species. [ABSTRACT FROM AUTHOR]

Human transformation of natural habitats facilitates pathogen transmission between domestic and wild species. The guigna (Leopardus guigna), a small felid found in Chile, has experienced habitat loss and an increased probability of contact with domestic cats. Here, we describe the interspecific transmission of feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) between domestic cats and guignas and assess its correlation with human landscape perturbation. Blood and tissue samples from 102 free‐ranging guignas and 262 domestic cats were collected and analyzed by PCR and sequencing. Guigna and domestic cat FeLV and FIV prevalence were very similar. Phylogenetic analysis showed guigna FeLV and FIV sequences are positioned within worldwide domestic cat virus clades with high nucleotide similarity. Guigna FeLV infection was significantly associated with fragmented landscapes with resident domestic cats. There was little evidence of clinical signs of disease in guignas. Our results contribute to the understanding of the implications of landscape perturbation and emerging diseases. [ABSTRACT FROM AUTHOR]

Feline morbillivirus infections have gained increased attention due to repeated reports of their association with urinary tract disease in cats. In the present study, 112 serum samples from free-roaming domestic cats in Chile were tested for antibodies against feline morbillivirus genotypes 1 and 2 (FeMV-1 and FeMV-2) using an indirect immunofluorescence assay. In total, 63% of the animals showed antibodies against one or both FeMV genotypes. Antibodies directed exclusively against FeMV-2 were significantly more prevalent in male cats. The correlation of sex and FeMV-2 infection might give insight into potential routes of transmission. We provide, for the first time, serological data on FeMV in Chile. [ABSTRACT FROM AUTHOR]

We present comments on an article recently published in Ecology and Evolution ("High‐resolution melting of the cytochrome B gene in fecal DNA: A powerful approach for fox species identification of the Lycalopex genus in Chile") by Anabalon et al. that reported the presence of Darwin's fox (Lycalopex fulvipes), a temperate forest specialist, in the hyperarid Atacama Desert of northern Chile. We argue that this putative record lacks ecological support in light of ongoing research on this endangered species, and contains numerous methodological flaws and omissions related to the molecular identification of the species. Based on these issues, we suggest the scientific community and conservation decision‐makers disregard the alleged presence of the Darwin's fox in the Atacama Desert. We present a Reply letter for a recent paper reporting the presence of Darwin´s fox, a forest‐specialist canid, in the Atacama Desert, 1,200 km north of its known distribution. [ABSTRACT FROM AUTHOR]

Owned, free-roaming domestic cats are abundant in the Chilean countryside, having high probability of contact with wildlife and potentially participating as reservoirs of zoonotic pathogens. In the present study, 131 cats from two remote study areas (Valdivia and Chiloe Island) in southern Chile were analyzed for infection/exposure to eight pathogens. Serum samples from 112 cats were tested for antigens against feline leukemia virus (FeLV antigen-ELISA) and antibodies against feline immunodeficiency virus (FIV-ELISA) and canine distemper virus (CDVserum neutralization), yielded occurrence of 8.9, 1.7 and 0.8% respectively. The presence of DNA of five vector-borne pathogens, piroplasmids, Ehrlichia spp., Anaplasma spp., Rickettsia spp. and Bartonella spp. was investigated in thirty cats. Overall observed occurrence was 6.6% (2/30) for both Anaplasma platys, and B. henselae, and 3.3% (1/30) for both Bartonella sp. and Theileria equi. Observed occurrence for all vector-borne pathogens in Valdivia area was significantly higher than in Chiloe Island (5/15 vs 0/15; P=0.04). Our results represent the first description of exposure to CDV and DNA detection of T. equi and A. platys in domestic cats in Chile. The results highlight the importance of performing pathogen screening in owned, free-roaming rural cats to evaluate their potential role as reservoirs of infection and vectors for disease transmission to wildlife. [ABSTRACT FROM AUTHOR]

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Simple Summary: The Andean cat is an endangered medium-sized felid with grey fur and a characteristic long tail with black rings. It is found only in cold and arid regions of the high Andes of Argentina, Bolivia, Chile, and Peru. As part of a collaboration between the Andean Cat Alliance (AGA) and Parque Andino Juncal (PAJ), we conducted a monitoring program from December 2020 to May 2021 using eight trail cameras in PAJ in the central Andes of Chile, an area where few records of the species have been described. We obtained records of Andean cats which fill a gap on the species distribution map, specifically in the area located between two previously identified evolutionary significant units (ESU) which are populations with different evolutionary histories that should be monitored, managed, and protected separately. Based on this new information, a comprehensive ESU reassessment across the range is currently being conducted by AGA. This will allow for a better understanding of the number of distinct populations across the range and the connectivity (i.e., current gene flow) among them, enabling the prioritization of small and isolated populations or units (i.e., higher extinction risk) and recommendations for evidence-based conservation strategies across the distribution. The Andean cat (Leopardus jacobita) is one of the most endangered and least known wild cat species in the Americas and the world in general. We describe new records of the Andean cat in the central Andes of Chile, in Parque Andino Juncal, obtained as part of a monitoring program conducted from December 2020 to May 2021 using eight trail cameras. The cameras were active for 135 days (sampling effort 1080 camera-trap days). We recorded Andean cats in two different cameras, corresponding to two independent events in January and March 2021, respectively (0.19% capture success). Our new records are relevant since they fill a gap on the species distribution map, specifically in the area located between the two previously identified evolutionarily significant units (ESU) (26–35° S) which has been prioritized by the Andean Cat Alliance (AGA). We highlight the relevance of private protected areas in ecosystems of high biodiversity and fragility such as Parque Andino Juncal and also of strategic private-social partnerships for successful collaborative efforts to monitor the presence of rare, elusive, and endangered species. Our next steps will be to collect scats from this newly identified site and conduct genetic analyses to determine whether these animals are part of previously identified ESUs or a yet unidentified potentially distinct ESU or MU requiring special conservation measures. [ABSTRACT FROM AUTHOR]

Blood samples of 626 rural dogs, 140 Andean foxes (Lycalopex culpaeus), and 83 South American grey foxes (L. griseus) from six bioregions of Chile spanning 3000 km were screened for Mycoplasma DNA by conventional PCR and sequencing. Risk factors of infection were inferred using Generalized Linear Mixed Models and genetic structure by network analyses. Overall, Mycoplasma haemocanis/Mycoplasma haemofelis (Mhc/Mhf) and Candidatus Mycoplasma haematoparvum (CMhp) observed prevalence was 23.8% and 12.8% in dogs, 20.1% and 7.2% in Andean foxes, and 26.5% and 8.4% in grey foxes, respectively. Both hemoplasmas were confirmed in all the bioregions, with higher prevalence in those where ticks from the Rhipicephalus sanguineus species group were absent. Candidatus M. haematominutum and a Mycoplasma sp. previously found in South American carnivores were detected in one fox each. Although the most prevalent Mhc/Mhf and CMhp sequence types were shared between dogs and foxes, network analysis revealed genetic structure of Mhc/Mhf between hosts in some regions. Male sex was associated with a higher risk of Mhc/Mhf and CMhp infection in dogs, and adult age with CMhp infection, suggesting that direct transmission is relevant. No risk factor was identified in foxes. Our study provides novel information about canine hemoplasmas with relevance in distribution, transmission routes, and cross-species transmission. [ABSTRACT FROM AUTHOR]

Simple Summary: The presence of carnivores near human settlements is a poorly studied topic that generates concern and perception of risk in some human communities, especially for medium to large felids. Apart from the conflict of the potential predation of livestock, there is the insecurity perception of a potential attack on people. To gain a better understanding of how, when, and how close pumas approached human settlements, we analyze 51 puma records near populated areas over eight years in central Chile. The results show that pumas approached human-populated areas; in 23.5% of the records pumas are found between 0 and 999 m from the nearest human settlement, 25.5% are between 1000 and 4999 m, and 51% are over 5000 m. We associate puma records with landscape features, such as mountain ranges, land-use, road, and urban infrastructure; and based on previous knowledge of puma biology, behavior, and habitat preference, we identify their area of occupation and the potential biological corridor used for their movements from the Andes Range to the coast. Our results show the adaptability of pumas to human-dominated landscapes, and their capacity to overcome landscape barriers, such as human infrastructure, contributing to a better understanding of the population dynamics in the study area. Studies on human–carnivore coexistence, through formulas that consider local realities and the reduction of implicit risks for humans, are urgently needed, both globally and locally, and likely the only way to secure the long-term conservation of pumas in human-dominated landscapes. The wildland–urban interface lies at the confluence of human-dominated and wild landscapes—creating a number of management and conservation challenges. Wildlife sightings near human settlements have appeared to increase in the last years. This article reports 51 records of presences, sightings, and livestock attacks of Puma concolor, a large-sized felid, collected from 2012 to 2020 across the O'Higgins region in central Chile. Puma records were concentrated in the east of the region in the Andes Range and foothills (90%). The number of puma records is higher in the last four to six years than in previously studied years. Of the 51 records, 23.5% are between 0 and 999 m from the nearest human settlement (classified as very close), 25.5% are between 1000 and 4999 m (moderately close), and 51% are over 5000 m (distant). Most of the sightings are recorded in the summer (35%) and spring (29%). We identify an area of approximately 9000 km2 of suitable habitat as the most probable corridor effectively connecting pumas moving between eastern and western areas, encompassing the Angostura de Paine mountain range. Our results contribute to the understanding of the presence and movements of P. concolor near urban areas and human settlements, confirming their persistence in and adaptation to human-dominated landscapes. We also provide insights into human–carnivore coexistence in the current global context in the densely populated central Chile. [ABSTRACT FROM AUTHOR]

The family of paramyxoviruses has received growing attention as several new species have been identified recently, notably two different clusters in domestic cats, designated as feline morbillivirus (FeMV) and feline paramyxovirus (FPaV). Their phylogenetic origin and whether wild felids also harbor these viruses are currently unknown. Kidney samples from 35 guignas (Leopardus guigna), a wild felid from Chile, were investigated for paramyxoviruses using consensus-RT-PCR. In addition, thirteen serum samples of guignas were screened for the presence of FeMV-specific antibodies by an immunofluorescence assay (IFA). Viral RNA was detected in 31% of the kidney samples. Phylogenetic analyses revealed two well-supported clusters, related to isolates from domestic cats, rodents and bats. No significant histopathology changes were recorded in infected guignas. Serology identified two samples which were positive for FeMV-specific antibodies. Our study highlights the diversity of paramyxovirus infections in felids with special emphasis on guignas from Chile. [ABSTRACT FROM AUTHOR]

We report new records of the wild felid Leopardus guigna in its northern-most distribution, in the southern Coquimbo and northern Valparaíso regions, in northern-central Chile. To our knowledge, these are the northern-most confirmed guigna records to date. We discuss implications for the conservation of these fragile populations in the face of different anthropic threats. [ABSTRACT FROM AUTHOR]

The article presents a study on parasites of wild and domestic carnivores, such as such as Hepatozoon spp., Babesia spp., Leishmania spp., Trypanosoma cruzi, and Spirocerca spp., of Chile. It reports that In Chile, there is limited or null information about their presence, although these parasites have been reported in neighboring countries. The study aimed to determine its presence and importance for dogs and wild foxes.

Background: Human activities are permanently threatening wildlife. Wildlife Rescue and Rehabilitation Centers (WRRC) have served for the rescue, rehabilitation and reinsertion of affected and recovered animals. Methods: We reviewed the casuistry of five wildlife rescue and rehabilitation centers in Chile over 5 years, and described the main causes of admission, most affected taxonomic groups and final outcomes of the admitted individuals, shedding light into general patterns and relevant factors currently affecting wildlife in Chile. To understand the current work and status of WRRC system in Chile, we also conducted a qualitative survey to WRRC personnel and Agricultural and Livestock Service (SAG) regional offices regarding their operation. Results: A total of 3418 cases of animals admitted to WRRC were obtained; 95.3% corresponded to native species. Of native animal cases, 86.0% corresponded to birds, 12.3% were mammals and 1.7% reptiles. Trauma was the most frequently observed cause of admission in all three native fauna groups (35.8% in birds, 23.2% in mammals, 27.8% in reptiles). Conclusions: WRRC are a tool for conservation and education of wild animal species in Chile, however WRRC and SAG regional office personnel highlighted several deficiencies in the current system and suggested opportunities for improvement. The current WRRC system needs modernization and financial support from the Chilean state to fulfil their relevant mission. [ABSTRACT FROM AUTHOR]

Antibodies against Spotted Fever Group (SFG) Rickettsia and Coxiella burnetii , investigated through indirect antibody immunofluorescence tests, were detected in serum samples from 3.1% and 0% of 358 rural dogs, respectively, and in none of 32 wild foxes tested. SFG Rickettsia seropositive dogs were only detected in the Mountain Desert (8%) and the Steppe-Mediterranean (9%) regions. Exposure in the Mountain Desert, where no ticks and fleas were found on any dog, could correspond to a new SFG Rickettsia sp. recently described in soft ticks or to a related agent. Our survey confirms low endemicity in the country of C. burnetii , as observed in recent serosurveys in humans. • Low seroprevalence to SFG Rickettsia in rural dogs from four Chilean bioregions. • Antibodies in high plateau dogs may correspond to a recently described Rickettsia. • All dogs seronegative for antibodies against Coxiella burnetii. • All wild foxes seronegative for antibodies against both agents. [ABSTRACT FROM AUTHOR]