1. Physiological evaluation of a new Chlorella sorokiniana isolate for its biomass production and lipid accumulation in photoautotrophic and heterotrophic cultures.
- Author
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Wan MX, Wang RM, Xia JL, Rosenberg JN, Nie ZY, Kobayashi N, Oyler GA, and Betenbaugh MJ
- Subjects
- Carbon metabolism, China, Chlorella isolation & purification, Chlorella metabolism, DNA, Plant chemistry, DNA, Plant genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Fresh Water microbiology, Heterotrophic Processes, Hydrogen-Ion Concentration, Molecular Sequence Data, Nitrogen metabolism, Phospholipids analysis, Phototrophic Processes, RNA, Ribosomal, 18S genetics, Sequence Analysis, DNA, Temperature, Biomass, Chlorella classification, Chlorella growth & development, Lipid Metabolism
- Abstract
A novel green unicellular microalgal isolate from the freshwater of the Inner Mongolia Province of China and named as CCTCC M209220, grows between pH 6 and 11 and temperatures of 20-35°C with optimal conditions at pH 9 and 30°C. Morphological features and the phylogenetic analysis for the 18S rRNA gene reveal that the isolate is a Chlorella sorokiniana strain. A nitrogen source test reveals that this strain can grow well with nitrate and urea, but not ammonium. The strain can grow heterotrophically with glucose as the carbon source and accumulates lipid content as high as 56% (w/w) dry weight after 7 days in high glucose concentrations compared to 19% lipids achieved in 30 days of photoautotrophic culture. The relative neutral lipid content as a fraction of the total lipid is also much higher in heterotrophic culture as compared to photoautotrophic culture., (Copyright © 2012 Wiley Periodicals, Inc.)
- Published
- 2012
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