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Haizhou Bay, a semi-enclosed key aquaculture area in East China, has had relatively limited research focused on trace metals and perfluoroalkyl acids (PFAAs) in its biota. This study characterized the distribution, biomagnification and health risks of selected trace metals and PFAAs in various marine organisms from Haizhou Bay. Among the species examined, zinc (Zn) was the most prevalent metal, followed by copper (Cu) and chromium (Cr), whereas cadmium (Cd), total mercury (THg), and methylmercury (MeHg) contents were relatively low. Perfluorooctane sulfonate (PFOS) was the most abundant PFAA, followed by perfluorooctanoic acid (PFOA). The calculated trophic magnification factors (TMFs) were above one for Cr, THg, MeHg, and all PFAAs except perfluorobutanoic acid (PFBA) and perfluorotetradecanoic acid (PFTeDA). Across animal groups, gastropods exhibited relatively low levels of THg, MeHg, and perfluorosulfonic acids (∑PFSAs). By comparison, fish generally had lower levels of Cd and Cu compared to other animal groups, and demersal fish had significantly higher MeHg compared to gastropods. Certain organisms, such as cephalopods and shrimps, were found to pose potential health risks due to elevated levels of Cd, while levels of other studied metals, PFOS and PFOA generally appeared to be within safe limits for human consumption. Further research is needed to assess the sources and impacts of these and other contaminants., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Erhai Lake, a highland lake situated in Southwest China, provides critical aquatic protein sources for the local community, and its preservation is vital due to the sensitivity of alpine freshwater ecosystems to disturbance. However, there is a lack of research on the contamination status of methylmercury (MeHg) in aquatic organisms of the Erhai Lake Basin. MeHg concentrations in important commercial fish species from the Erhai Lake were examined, and the potential health risks associated with human consumption were assessed. Our results showed significant inter-species differences in fish muscle MeHg: the carnivorous S. asotus exhibited the highest level (303 ng/g; ww), while that of the detritivorous R. ocellatus was the lowest (3.86 ng/g). Moreover, MeHg concentrations in P. fulvidraco and C. auratus collected from the Luoshi River (a major tributary of Erhai Lake) were significantly higher compared to those from the Erhai Lake, indicating possible river-based input of MeHg into the Erhai Lake. Additionally, our study revealed a significant positive correlation between the MeHg levels and the length as well as weight of the examined fish species. All the fish species analyzed in our study had MeHg concentrations within the limits of China's food safety standard. Nevertheless, a relatively low consumption quantity of 16 g per day of certain species (i.e., S. asotus) may still pose potential health risks especially for children. The present study provides baseline data for MeHg monitoring and risk assessment in the Erhai Lake Basin, and warrants continued monitoring and source investigation., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

China, the largest developing country, has experienced rapid urbanization since its reform and opening-up. However, the increasing pollution load from urban areas has deteriorated urban river water quality, contradicting the concept of sustainable and green development promoted by the Chinese government. This situation elucidates governmental shortcomings in systematic environmental protection. Our study revealed that the current wastewater treatment plant (WWTP) discharge standards in urban areas are insufficient for attaining the desired urban river water quality and thus intensify the conflict between urbanization and water environmental protection. As urbanization continues, the urban population will grow, further exacerbating pollution and conflict. Our focus was the Xiangjiang River basin in Zunyi, a typical urbanized city in China. Using a validated one-dimensional mathematical model, we compared the water quality in the Xiangjiang River between current and upgraded WWTP discharge standards. The results showed that the water quality in the Xiangjiang River falls short of the standards, with more than 60 % of the river exceeding limits. However, upgrading WWTP discharge standards significantly reduces the proportion of river sections exceeding limits, with only 0.4 % exceeding standards during specific periods. This enhancement greatly improved the Xiangjiang River's water quality, aided in restoring the entire water environment in the basin, and supported water environmental protection goals. Our research findings offer crucial support for local governments in shaping comprehensive water environmental protection policies and insights for addressing similar environmental challenges caused by rapid urbanization in other developing regions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Background: The status of children's early motor skills play an important role during childhood and across lifetime. This study described FMS proficiency among boys (n = 189) and girls (n = 179) kindergarten children from 3 to 6 years old (4.4 s 0.7, mean ± SD) in northwest China. The differences in FMS proficiency of boys and girls from different environments, ethnic groups were analyzed respectively., Methods: TGMD-3 was used to assess FMS. FMS mastery level was defined according to the correct performance of all criteria over two trials. The correlation between BMI and FMS and the interaction of environmental and ethnic on FMS were analyzed. The general linear model was used to evaluate the differences of boys and girls among environment groups (urban/suburban/county), and ethnic groups (Han/Hui/Tibetan) on the FMS subsets respectively., Results: FMS proficiency was assessed in 368 3- to 6-year-old children (n = 156 urban, n = 101 suburban, n = 111 county)/(n = 208 Han, n = 107 Hui, n = 53 Tibetan). Overall, the highest skill performance was the run, with 86% achieving mastery level, and the poorest performance was the FH strike, at only 19%. Correlation between BMI and FMS is minimal. According to TGMD-3 scores, there was no significant difference between boys and girls in total FMS (p = 0.38). In terms of locomotor skills, boys performed better than girls in the hop, skip and slide (p < 0.05). County children performed significantly difference than urban and suburban children. Some skills performed less proficiently, (boys in 6 of 13 skills: run, HJ, slide, TH strike, FH strike and kick; girls in 4 of 13 skills: run, slide, TH strike and kick) and some skills performed more proficiently (boys in dribble; girls in hop and dribble). Tibetan children performed significantly difference than Han and Hui children. Some skills performed less proficiently, (boys in 6 of 13 skills: run, HJ, slide, TH strike, FH strike and kick; girls in TH strike) and some skills performed more proficiently (boys and girls were all in dribble)., Conclusion: Children in northwest China showed certain characteristics in FMS, the county/Tibetan boys and girls performed poorer than others in ability to execute particular process characteristics of some skills and performed more outstanding in other skills. It suggests that a certain group population may need specific focus on interventions to improve their FMS level., (© 2022. The Author(s).)

The effect of ambient temperature on dermatologic diseases has received widespread attention. Previous studies have shown that ambient temperature might affect specific dermatologic diseases, but results were inconsistent. This study aims to assess the short-term effect of ambient temperature on outpatient visits due to dermatologic diseases (DMs) in Xinxiang, China. Daily DMs outpatient visits, mean temperature, mean relative humidity, and air pollution data of Xinxiang were retrieved from January 1, 2015, to December 31, 2018. A distributed lag nonlinear model (DLNM) was applied to analyze the effect of ambient temperature on DMs outpatients. We controlled several potential confounding factors such as the long-term trend, public holiday, day of the week, humidity, and air pollutants (NO 2 , PM 2.5 ). Finally, two more stratification analysis was conducted by age and gender. A total of 164,270 outpatients of DMs were enrolled during our study, and the daily mean visits were 113. The estimated effect of temperature on DMs was nonlinear. Heat temperature would exacerbate outpatients of dermatologic diseases. With a reference median temperature (17 °C), the effect of temperature on DMs was most pronounced at lag0-14; exposure to heat (32 °C, 99th) was associated with 1.565 (95% CI: 1.266-1.934) increased risk of outpatients for DMs. Stratification analysis showed that citizens of young ages were susceptive to heat; both genders had a similar relationship between temperature and DMs risk. This study highlights that ambient temperature was associated with DMs outpatients; heat temperature might aggravate DMs risk. The health hazards of heat temperature required more attention, and more effective prevention measurements should be designed and implemented to curb global warming., (© 2022. The Author(s) under exclusive licence to International Society of Biometeorology.)

The study was based on the teaching effect of blended teaching of the course of Veterinary Immunology on the U-Massive Open Online Course (UMOOC) platform of Inner Mongolia Minzu University. The students of two classes majoring in veterinary medicine in Inner Mongolia Minzu University were selected to form the traditional teaching group (control group) and the blended teaching group (experimental group), respectively, and the teaching effects were further compared and analyzed. The blended teaching module improved students' active learning attitude along with their ability to grasp knowledge in class, and most students supported to use blended teaching in the future. In conclusion, the UMOOC-based blended teaching methodology stimulated students' active learning attitude toward veterinary immunology and helped to foster innovative consciousness and self-management ability.

Previous studies have reported regional variations in the relationship between ambient temperature and dermatitis, which therefore remain inconclusive. This study aimed to understand the short-term effects of ambient temperature in outpatients with dermatitis in Xinxiang. Routine data of dermatitis outpatient visits between January 1, 2015, and December 31, 2018 were acquired from the First Affiliated Hospital of Xinxiang Medical University. A distributed lag nonlinear model (DLNM) in combination with a generalized additive model (GAM) was applied to investigate the acute impact of ambient temperature on dermatitis outpatients. Two stratified analyses based on age and sex were conducted to explore their potential modification effects. A total of 18,064 dermatitis outpatient records were retrieved during the study period. The relationship between ambient temperature and dermatitis outpatients was found to be nonlinear; exposure-response curves were approximately "J" shaped. The risk of dermatitis outpatient increased with a corresponding elevation in temperature from 11.5 to 27.5°C. However, the significance was lost when the temperature exceeded the given range. Stratified analysis demonstrated higher sensitivity to temperature in females than in males, and the young groups (<15 years) might be sensitive to heat temperature. This study provides first-hand epidemiological evidence demonstrating a higher risk of dermatitis outpatient visits associated with moderately higher temperatures. The outcomes of this study create awareness among citizens to take the necessary precautions to prevent the adverse effects of moderate heat temperature during early summer and autumn seasons., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Background: Dietary patterns and symptoms research among Chinese with esophageal squamous cell carcinoma (ESCC) and its precursor lesions is limited, especially as it relates to multiple food consumption and multiple co-occurring symptoms. The aim of our study was to identify the dietary patterns and severity of symptom classes with the risk of esophageal squamous cell carcinoma and its histological precursor lesions, and develop a risk prediction model for different stages of esophageal disease., Methods: We analyzed data from a multicenter cross-sectional study carried out in ESCC high incidence areas between 2017 and 2018, which included 34,707 individuals aged 40-69 years. Dietary patterns and severity of symptom classes were derived by applying a latent class analysis (LCA). A multiple logistic regression model was used to derive the odds ratio (ORs) and corresponding 95% confidence intervals (CIs) for ESCC and the different stages of esophageal disease according to the dietary patterns and severity of symptom classes identified. We built the risk prediction model by using a nomogram., Results: We identified five dietary patterns and three severity of symptom classes. The dietary patterns were classified as follows: "Healthy", "Western", "Lower consumers-combination", "Medium consumers-combination" and "Higher consumers-combination" patterns based on the intake of foods such as red meat, vegetables and fruits. The severity of symptoms was categorized into "Asymptomatic", "Mild symptoms" and "Overt symptoms" classes based on health-related symptoms reported by the participants. Compared to the "Healthy" pattern, the other four patterns were all associated with an increased risk of esophageal disease. Similarly, the other two symptom classes present different degrees of increased risk of esophageal disease compared to the "Asymptomatic". The nomograms reflect the good predictive ability of the model., Conclusion: Among individuals aged 40-69 years in high incidence regions of upper gastrointestinal cancer, the results supplied that subjects with diets rich in livestock and poultry meat and low in fruits and vegetables and subjects with typical symptoms were at increased ESCC risk. The findings highlight the importance of considering food and symptom combinations in cancer risk evaluation., (© 2022. The Author(s).)

Background: Despite research efforts, the causative factors that contribute to esophageal squamous cell carcinoma (ESCC) in high-risk areas have not yet been understood. In this study, we, therefore, aimed to describe the risk factors associated with ESCC and its precursor lesions., Methods: We performed an endoscopic examination of 44,857 individuals aged 40-69 years from five high incidence regions of China in 2017-2018. Participants were classified as 4 groups of normal control, esophagitis, low-grade intraepithelial neoplasia (LGIN) and high-grade intraepithelial neoplasia/esophageal squamous cell carcinoma (HGIN/ESCC) using an unconditional logistic regression determine risk factors., Results: We identified 4890 esophagitis, 1874 LGIN and 437 HGIN/ESCC cases. Crude odds ratios (ORs) and adjusted odds ratios were calculated using unconditional logistic regression. Drinking well and surface water, salty diet, and positive family history of cancer were the common risk factors for esophagitis, LGIN and HGIN/ESCC. History of chronic hepatitis/cirrhosis was the greatest risk factor of esophagitis (adjusted OR 2.96, 95%CI 2.52-3.47) and HGIN/ESCC (adjusted OR 1.91, 95%CI 1.03-3.22). Pesticide exposure (adjusted OR 1.20, 95%CI 1.05-1.37) was essential risk factor of LGIN., Conclusions: Among individuals aged 40-69 years in high incidence regions of upper gastrointestinal cancer, the results provided important epidemiological evidence for the prevention of different precancerous lesions of ESCC., (© 2021. The Author(s).)

Objectives: As the largest organ of the human body, the skin is the major exposure route of NO 2 . However, the evidence for a relationship between NO 2 exposure and dermatologic diseases (DMs) is limited. This time-series study was conducted to assess the short-term effect of nitrogen dioxide (NO 2 ) exposure on DMs outpatient visits in Xinxiang, China., Methods: Daily recordings of NO 2 concentrations, meteorological data, and the outpatient visits data for DMs were collected in Xinxiang from January 1st, 2015, to December 31st, 2018. The analysis method used was based on the generalized additive model (GAM) with quasi-Poisson regression to investigate the relationship between NO 2 exposure and DMs outpatient visits. Several covariates, such as long-term trends, seasonality, and weather conditions were controlled., Results: A total of 164,270 DMs outpatients were recorded. A 10 μg/m 3 increase in NO 2 concentrations during the period was associated with a 1.86% increase in DMs outpatient visits (95% confidence intervals [Cl]: 1.06-2.66%). The effect was stronger (around 6 times) in the cool seasons than in warmer seasons and younger patients (< 15 years of age) appeared to be more vulnerable., Conclusions: The findings of this study indicate that short-term exposure to NO 2 increases the risk of DMs in Xinxiang, China, especially in the cool seasons. Policymakers should implement more stringent air quality standards to improve air quality., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.)

Blastocystis is a common enteric protist that inhabits the gastrointestinal tract of approximately 1 billion people worldwide. In this study, a total of 1,070 patients from two hospitals in Zhengzhou, Central China were enrolled to know molecular characteristics of Blastocystis sp. The microorganism was identified and subtyped with a PCR amplification and sequencing of the small subunit ribosomal deoxyribonucleic acid (SSU-rDNA). The overall minimum prevalence of Blastocystis sp. in participants was 3.1% (33/1070). Although there were no significant differences on Blastocystis sp. infections among study sites, age groups, and gender, the higher infection was observed in the patients with gastrointestinal diseases (8.8%, 15/170). Sequence analysis of the 33 isolates revealed three known subtypes, such as ST1 (n = 7), ST3 (n = 23), and ST7 (n = 3). Among them, ST3 was the dominant subtype being detected in 23 isolates (69.7%), followed by ST1 (21.2%, 7/33) and ST7 (9.1%, 3/33). The phylogenetic analysis demonstrated that three subtypes (ST1, ST3 and ST7) were clustered with their reference sequences with good bootstrap support. The subtype determination of Blastocystis sp. isolates by the phylogenetic analysis was well supported by online platform. The present study provides the first molecular report of Blastocystis sp. infections in hospital patients in Central China., (Copyright © 2021 Elsevier GmbH. All rights reserved.)

Cryptosporidium and Giardia are ubiquitous protozoan parasites that infect a broad range of hosts. The presence of Cryptosporidium spp. and G. duodenalis was detected in 355 fecal samples of laboratory experimental rats from four experimental rat rearing facilities in China by PCR amplification of the small subunit (SSU) rRNA gene. The G. duodenalis positive samples were further characterized in the β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes. The overall infection rates of Cryptosporidium spp. and G. duodenalis were 0.6% (2/355) and 9.3% (33/355), respectively, with no co-infection. Among the four facilities, only the rats in Zhengzhou1 were found positive for the two pathogens. Undetermined Cryptosporidium genotype was observed in one sample and C. ubiquitum in another sample. Assemblage G was identified in all the 33 G. duodenalis positive isolates at SSU rRNA gene, out of which 19, 20, and 21 isolates were also subtyped as assemblage G at tpi, gdh and bg gens, respectively. To our knowledge, this is the first report of Cryptosporidium and G. duodenalis infections in laboratory experimental rats in China. The infections of these pathogens in laboratory animals should be monitored routinely since they may interfere the biological experiments in these animals., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Objective: To investigate the predictive value of early indicators changes in blood test on the prognosis of patients with acute paraquat poisoning., Methods: The clinical data of patients with acute paraquat poisoning admitted to emergency department of Shengjing Hospital of China Medical University from January 2012 to June 2019 were retrospectively analyzed. The changes of blood test indexes within 24 hours after admission were collected, including white blood cell count (ΔWBC), neutrophils count (ΔNE), lymphocytes count (ΔLY), monocytes count (ΔMO), arterial partial pressure of oxygen (ΔPaO 2 ), arterial partial pressure of carbon dioxide (ΔPaCO 2 ), arterial blood pH (ΔpH), bicarbonate radical (ΔHCO 3 - ), base excess (ΔBE), lactate (ΔLac), total protein (ΔTP), albumin (ΔALB), alanine aminotransferase (ΔALT), aspartate aminotransferase (ΔAST), total bilirubin (ΔTBil), direct bilirubin (ΔDBil), blood urea nitrogen (ΔBUN), serum creatinine (ΔSCr), serum calcium concentration (ΔCa 2+ ), and serum potassium concentration (ΔK + ). Multivariate Logistic regression was used to analyze the risk factors of prognosis in patients with acute paraquat poisoning, and receiver operating characteristic (ROC) curve was drawn to analyze the predictive value of ROC curve for the death of patients with paraquat poisoning., Results: A total of 251 patients with acute paraquat poisoning were included, with 99 cases dead, and the mortality was 39.4%. The increase of the markers including ΔWBC, ΔLac, ΔALT, ΔAST, ΔTBil, ΔDBil, ΔBUN, ΔSCr and ΔK + within 24 hours of admission in the death group were significantly higher than that in the survival group; the decrease of the markers including ΔPaCO 2 , ΔHCO 3 - , ΔBE, ΔTP, and ΔALB in the death group were significantly greater than those in the survival group. The variables with statistical significance in the above single factor analysis were included in the multivariate Logistic regression analysis. The results showed that ΔLac, ΔSCr and ΔK + for predicting death of patients with acute paraquat poisoning was 0.639 (95%CI was 0.505-0773), 0.811 (95%CI was 0.704-0.917), and 0.649 (95%CI was 0.519-0.779), respectively. When the cut-off of ΔLac was 1.85 mmol/L, the sensitivity was 87.9%, the specificity was 47.7%, and the diagnostic accuracy was 70.2%; when the cut-off of ΔSCr was 37.75 μmol/L, the sensitivity was 84.4%, the specificity was 77.9%, and the diagnostic accuracy was 80.5%; when the cut-off of ΔK + was 0.42 mmol/L, the sensitivity was 36.6%, the specificity was 90.7%, and the diagnostic accuracy was 68.3%. The efficiency of combination of ΔLac, ΔSCr, and ΔK + was 0.42 mmol/L, the sensitivity was 36.6%, the specificity was 90.7%, and the diagnostic accuracy was 68.3%. The efficiency of combination of ΔLac, ΔSCr, and ΔK + was greater than a single indicator in predicting death of patients with acute paraquat poisoning, with AUC of 0.911, and 95%CI of 0.834-0.989., Conclusions: ΔLac, ΔSCr, ΔK + within 24 hours of admission were all independent risk factors for the prognosis of patients with acute paraquat poisoning. ΔSCr > 37.75 μmol/L within 24 hours of admission would predict a poor prognosis in the patients with acute paraquat poisoning. Combined analysis of ΔLac, ΔSCr, and ΔK + can predict the prognosis of paraquat poisoning patients more accurately than single index.

Retinal fundus photography provides a non-invasive approach for identifying early microcirculatory alterations of chronic diseases prior to the onset of overt clinical complications. Here, we developed neural network models to predict hypertension, hyperglycemia, dyslipidemia, and a range of risk factors from retinal fundus images obtained from a cross-sectional study of chronic diseases in rural areas of Xinxiang County, Henan, in central China. 1222 high-quality retinal images and over 50 measurements of anthropometry and biochemical parameters were generated from 625 subjects. The models in this study achieved an area under the ROC curve (AUC) of 0.880 in predicting hyperglycemia, of 0.766 in predicting hypertension, and of 0.703 in predicting dyslipidemia. In addition, these models can predict with AUC>0.7 several blood test erythrocyte parameters, including hematocrit (HCT), mean corpuscular hemoglobin concentration (MCHC), and a cluster of cardiovascular disease (CVD) risk factors. Taken together, deep learning approaches are feasible for predicting hypertension, dyslipidemia, diabetes, and risks of other chronic diseases., Competing Interests: The authors declare no competing interests.

Carbon (C) plays a vital role in regulating soil nutrient cycling and increasing soil microbial community, but there is still limited information on how C source utilization characteristics responds to soil physical and chemical properties changes under double-cropping rice (Oryza sativa L.) paddy field in southern China. Therefore, the effects of different short-term manure nitrogen (N) input rate managements on C source utilization characteristics in rice rhizosphere and non-rhizosphere soils under double-cropping rice field in southern China were studied by using 18 O-H 2 O method. Therefore, a field experiment were established in Ningxiang city of Hunan Province, and five different fertilizer treatments were applied: (1) 100% N of chemical fertilizer (M0), (2) 30% N of organic manure and 70% N of chemical fertilizer (M30), (3) 50% N of organic manure and 50% N of chemical fertilizer (M50), (4) 100% N of organic manure (M100), and (5) without N fertilizer input as control (CK). The results showed that soil microbial biomass C content, soil microbial growth rate, and soil microbial basal respiration with application of organic manure treatments (M30, M50, M100) were significantly higher (p < 0.05) than that of CK treatment. And the soil C utilization efficiency with M0 treatment were significantly higher (p < 0.05) than that of M100 treatment. Compared with CK and M0 treatments, the metabolic capacity of soil microorganisms to exogenous C sources with M30, M50 and M100 treatments were increased. The largest types of exogenous C source was carboxylic acids, followed by amino acid and carbohydrate, and complex compounds was the smallest. The RDA analysis results indicated that fertilizer treatments significantly changed the utilization characteristics of soil microorganisms to exogenous C sources. As a result, this study found that characteristics of soil C source utilization were significantly affected by different short-term manure N input rate managements.

Background: Dexmedetomidine is widely used for non-invasive pediatric procedural sedation. However, the hemodynamic effects of intravenous dexmedetomidine are a concern. There has been a growing interest in the application of intranasal dexmedetomidine as a sedative in children., Objective: To investigate the incidence of bradycardia in children undergoing intranasal dexmedetomidine sedation and to identify the associated risk factors., Methods: Data pertaining to pediatric patients who underwent intranasal dexmedetomidine sedation for non-invasive investigations at the Kunming Children's Hospital between October 2017 and August 2018 were retrospectively analyzed., Results: Out of 9984 children who qualified for inclusion, 228 children (2.3%) developed bradycardia. The incidence of bradycardia in the group that received additional dose of dexmedetomidine was higher than that in the group that did not receive additional dose (9.2% vs 16.7%; P = .003). The incidence of bradycardia in males was higher than that in females (2.6% vs 1.8%; P = .007). On multivariate logistic regression, only male gender showed an independent association with the occurrence of bradycardia (odds ratio 1.48; 95% confidence interval 1.11-1.97; P = .008)., Conclusions: The overall incidence of bradycardia in children after sole use of intranasal dexmedetomidine sedation was 2.3%. Male children showed a 1.48-fold higher risk of bradycardia. However, the blood pressure of the children who developed bradycardia was within the normal range. Simple wake-up can effectively manage bradycardia induced by intranasal dexmedetomidine sedation., (© 2019 The Acta Anaesthesiologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.)

Experimental rats are important animal models, and a history of pathogenic infections in these animals will directly affect the animal trial results. Enterocytozoon bieneusi is a ubiquitous potential pathogen transmitted via the fecal-oral route. To determine the prevalence and genotypic distributions of E. bieneusi in experimental rats in China, 291 fresh fecal samples were collected from four medical experimental animal centers. Enterocytozoon bieneusi was screened via nested-PCR amplification of the internal transcribed spacer (ITS) region. Of the rats tested, 4.8% (14/291) were positive for E. bieneusi. Five E. bieneusi ITS genotypes (four known: EbpA, EbpC, CHY1, and N; one novel: SHR1) were detected among 14 sequenced samples. The dominant E. bieneusi genotype was EbpA (50.0%, 7/14). In the phylogenetic analysis, genotypes EbpA and EbpC belonged to the previously described group 1, genotypes N and SHR1 belonged to group 2, and genotype CHY1 belonged to the novel group 12. To our knowledge, this is the first report of E. bieneusi in experimental laboratory rats in China. Infections with this pathogen must be monitored in laboratory animals, and quality control officers in the medical experimental centers should attempt to trace the pathogen's source and stop its transmission., Competing Interests: Declaration of competing interest The authors declare that they have no conflicts of interest., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Blastocystis sp. is a ubiquitous protist that has been frequently reported in humans and animals worldwide. A total of 355 fecal samples of experimental rats were collected from four laboratory rearing facilities in China, and Blastocystis sp. was detected by PCR amplification of the partial small subunit ribosomal (SSU) rRNA gene. Twenty-nine (8.2%, 29/355) samples were positive for Blastocystis sp., with the highest infection rate (20.7%, 24/116) in rats of the Zhengzhou1, followed by that in the Zhengzhou2 (5.0%, 2/40), Shenyang (3.0%, 3/100) and Wuhan (0) rearing facilities. Among the three rat strains, Sprague-Dawley (SD) rats had higher infection rates (11.3%, 17/151) compared to Wistar rats (8.7%, 9/104) and spontaneously hypertensive (SH) rats (3.0%, 3/100). Two Blastocystis sp. subtypes (ST4 and ST7) were identified. ST4 was the predominant subtype detected in 26 samples (89.7%). A phylogenetic analysis demonstrated that the sequences of ST4 and ST7 obtained in this study were clustered with their reference subtypes. To our knowledge, this is the first report of Blastocystis sp. in experimental rats in China. Pathogen infections in laboratory animals need to be monitored due to fecal-oral transmission., (© J. Li et al., published by EDP Sciences, 2020.)

Background: Bone mineral density (BMD) has been positively associated with lung function in patients diagnosed with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis. However, the relationship between BMD and lung function is inconsistent in the general population., Methods: To investigate the association between BMD and lung function in a Chinese general population, a total of 1024 adults aged 40-70 years old from Qiliying (an industrial polluted exposure area) and Langgongmiao (the reference area with non-industrial pollution) were recruited and underwent BMD and spirometry tests., Results: Both BMD and lung function levels were lower in the exposed area compared to the reference area. In addition, BMD and lung function levels were also lower in females compared to males. Both Spearman and partial correlation analyses showed that BMD was positively correlated with FVC and FEV 1 . After adjusting linear regression analyses for potential confounding factors, every 0.1 g/cm 2 drop in BMD was associated with 53.0 mL decrease in FVC and 33.5 mL decrease in FEV 1 ., Conclusions: A reduction of BMD is associated with lower lung function in a general population from China.

Few studies have investigated the acute exacerbations of chronic obstructive pulmonary disease (AECOPD)-associated attributable burden under exposure to high levels of air pollution among Asians. Data on hospitalization for AECOPD, air pollution and meteorological factors from 1 January 2013 to 31 December 2016 were collected in Shijiazhuang, China. We used a Poisson generalized linear regression model combined with a distributed lag nonlinear model (DLNM) to evaluate the relative cumulative risk for a lag of 0-7 days and examined the potential effect modifications by age and sex via stratification analyses, controlling for long-term trends, seasonal patterns, meteorological factors, and other possible confounders. Then, we computed hospitalization percentages attributable to air pollutants. The AECOPD-associated relative cumulative risks for PM 2.5 , PM 10 , NO 2 , SO 2 , and CO for a lag of 0-7 days were significantly positively correlated with hospitalization. The associations were stronger in females and retired patients. The NO 2 Cum RR of AECOPD admission was the greatest. A 10μg/m 3 increase in daily NO 2 concentration was associated with 6.7% and 5.7% increases in COPD hospitalizations in the retired and female groups, respectively. The results showed that 13%, 9.4%, 1.7%, 9.7%, and 8.8% of AECOPD hospitalizations were attributable to exposure to PM 2.5 , PM 10 , SO 2 , NO 2 , and CO, respectively. If the air pollutant concentration was reduced to the 24-h average grade II levels of NAAQS of China, the AECOPD attributable percentage for PM 2.5 and PM 10 would decrease by 80%. The air pollutants PM 2.5 , PM 10 , SO 2 , NO 2 , and CO were significantly relevant to AECOPD-associated hospitalization. The associations differed by individual characteristics. The retired and female populations were highly vulnerable.

Few researches have been investigated on the effects of ambient air pollutants from coal combustion on acute exacerbation of chronic obstructive pulmonary disease (AECOPD) hospitalizations. The whole time series was split into heating season and non-heating season. We used a quasi-Poisson generalized linear regression model combined with distributed lag non-linear models (DLNMs) to estimate the relative cumulative risk and calculate the air pollutant hospitalization burden of AECOPD for lag 0-7 days in heating season and non-heating season. There were higher PM 2.5 , PM 10 , NO 2 , SO 2 , and CO concentrations in heating seasons than non-heating season in Shijiazhuang; however, O 3 was higher in non-heating season than heating season. The AECOPD-associated relative cumulative risks for PM 2.5 , PM 10 , NO 2 , and SO 2 for lag 0-7 days were significantly positively associated with hospitalization in heating and non-heating season; we found that the cumulative relative risk of NO 2 was the greatest in every 1 unit of air pollutants during the heating season and the cumulative relative risk of SO 2 was the greatest during the non-heating season. The results showed that 17.8%, 12.9%, 1.7%, 16.7%, and 10.5% of AECOPD hospitalizations could be attributable to PM 2.5 , PM 10 , SO 2 , NO 2 , and CO exposure in heating season, respectively. However, the results showed that 19.5%, 22.4%, 15%, 8.3%, and 10.4% of AECOPD hospitalizations could be attributable to PM 2.5 , PM 10 , SO 2 , NO 2, and O 3 exposure in non-heating season, respectively. The attributable burden of AECOPD hospitalization in heating season and non-heating season are different. PM 2.5 , PM 10 , NO 2 , and CO are the main factors of heating season, while PM 10 , PM 2.5 , SO 2 , and O 3 are the main factors of non-heating season. In conclusions, the centralized heating can change the influence of attributable risk. When government departments formulate interventions to reduce the risk of acute hospitalization of chronic obstructive pulmonary disease (COPD), the influence of heating on disease burden should be considered.

While increasing evidence suggests that ozone (O 3 ) exposure is associated with adverse birth outcomes, only one study has focused on its impact on the premature rupture of membranes (PROM). Therefore, we thus examined the effect of O 3 on PROM in Xinxiang, China, using an over-dispersed Poisson generalized additive model. Several confounding factors, including meteorological factors, temporal trends, the day of the week, and public holidays, were considered in the model. We identified a total of 3255 instances of PROM from January 1, 2015 to December 31, 2017, and there was a significant association between the daily maximum 8-h mean concentrations (O 3-8h ) and PROM. Each 10 μg/m 3 increase in the 3-day average concentration (lag02) of O 3-8h corresponded to an increment in PROM of 5.42% (95% CI: 1.45-9.39%). Although the results of the stratified analyses were insignificant, a few trending results were observed: stronger associations between O 3 and PROM would occur in women with advanced age (≥35) or during the warm season than those in younger women (<35) or during the cool season. Our study indicates that O 3 exposure is an important risk factor of PROM and should be considered in its prevention and control in the study area., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Background: Previous studies have found associations between asthma morbidity and air pollution especially in young population, (PLoS One 12:e0180522, 2017; Can J Public Health 103:4-8, 2012; Environ Health Perspect 118:449-57, 2010; Am J Respir Crit Care Med 182:307-16, 2010; J Allergy Clin Immunol 104:717-22, 2008; J Allergy Clin Immunol 104:717-22, 1999; Environ Res 111:1137-47, 2011) but most of them were conducted in areas with relatively low air pollutant level. Moreover, very few studies have investigated the effect and burden modification of heating season during which the ambient air pollution level is significantly different from that during non-heating season in north China., Objectives: This study aimed to evaluate the effect and burden modification of heating on short-term associations between adult asthma hospitalizations and ambient air pollution in the north China city of Shijiazhuang., Methods: Generalized additive models combined with penalized distributed lag nonlinear models were used to model associations between daily asthma hospitalizations and ambient air pollutants from 1 January 2013 to 16 December 2016 in Shijiazhuang city, adjusting for long-term and seasonality trend, day of week, statutory holiday, daily mean air pressure and temperature. Attributable risks were calculated to evaluate the burden of asthma hospitalizations due to air pollutants exposure. The effect of pollutants on hospitalization and the attributable measures were estimated in heating and non-heating season separately and the comparisons between the two seasons were conducted., Results: All pollutants demonstrated positive and significant impacts on asthma hospitalizations both in heating season and non-heating season, except for O 3 in heating season where a negative association was observed. However, the differences of the pollutant-specific effects between the two seasons were not significant. SO 2 and NO 2 exposure were associated with the heaviest burden among all pollutants in heating season; meanwhile, PM 10 and PM 2.5 were associated with the heaviest burden in heating season., Conclusions: In conclusion, we found evidence of the effect of ambient air pollutants on asthma hospitalizations in Shijiazhuang. The central heating period could modify the effects in terms of attributable risks. The disease burden modification of heating should be taken into consideration when planning intervention measures to reduce the risk of asthma hospitalization.

The identification of phosphorus (P) fractions is essential for understanding the transformation and availability of P in paddy soils. To investigate the soil P fractions associated with soil properties under long-term fertilization, we selected three fertilization treatments, including no fertilization (CK), chemical fertilizers (NPK) and chemical fertilizers combined with manure (NPKM), from three long-term experiments located in Nanchang (NC), Jinxian (JX) and Ningxiang (NX). The results showed that chemical fertilizers combined with manure (NPKM) significantly (P ≤ 0.05) increased the soil total phosphorus, Olsen P and soil organic matter (SOM) by 2, 3 and 1 times, respectively, compared with the NPK treatment, and by 4, 17 and 2 times, respectively, compared with the CK treatment. NPKM significantly increased the grain yield compared with CK and NPK at all sites. The apparent P balance with NPK was higher in NC and NX but lower in JX compared with NPKM. Hedley fractionation revealed the predominance of most of the organic and inorganic phosphorus (Po and Pi) fractions with long-term fertilization, especially with the NPKM treatment, at all sites. The nonlabile P pool decreased by 14% and 18% whereas the moderately labile P pool proportions increased by 3 and 6 times with the NPK and NPKM treatments, respectively, compared to the CK treatment. The labile P pool showed a significant positive relationship with the SOM, total P and Olsen P contents. The moderately labile P was positively correlated with the total P and Olsen P. A significant positive correlation was observed between soil pH and the nonlabile P pool. Redundancy analysis revealed that the moderately labile P fraction (HCl dil. Pi fraction) was remarkably increased by the NPKM treatment and significantly correlated with the soil pH and total P concentration. The labile P fraction (NaHCO3-Pi) showed a strong relationship with the Olsen P and total P. However, the residual P fraction was negatively correlated with the HCl. dil. Pi fraction. We concluded that NPKM application improved P availability by many folds compared to NPK, which could lead to environmental pollution; therefore, the rate of combined application of manure and chemical fertilizer should be reduced compared to chemical fertilizer inputs to minimize the wastage of resources and environmental P losses., Competing Interests: The authors have declared that no competing interests exist.

Although numerous studies have investigated the association between air pollution and hospitalization, few studies have focused on the health effect of air pollution on populations with hypertension. In this study, we conducted a time-series study to investigate the acute adverse effect of six criteria ambient air pollutants (fine particulate matter [PM 2.5 ], inhalable particulate matter [PM 10 ], nitrogen dioxide [NO 2 ], sulfur dioxide [SO 2 ], ozone [O 3 ], and carbon monoxide [CO]) on hospitalization of patients for hypertension in Shijiazhuang, China, from 2013 to 2016. An over-dispersed Poisson generalized addictive model adjusting for weather conditions, day of the week, and long-term and seasonal trends was used. In addition, we evaluated the effect of modification by season, sex, and age. A total of 650,550 hospitalization records were retrieved during the study period. A 10 μg/m 3 increase of PM 2.5 (lag06), PM 10 (lag06), NO 2 (lag03), O 3 (lag6), and CO (lag04) corresponded to 0.56% (95% confidence interval [CI]: 0.28-0.83%), 0.31% (95% CI: 0.12-0.50%), 1.18% (95% CI: 0.49-1.87%), 0.40% (95% CI: 0.09-0.71%), and 0.03% (95% CI: 0.01-0.05%) increments in hospitalization of patients for hypertension, respectively. We observed statistically significant associations with PM 2.5 , PM 10 , NO 2 , O 3 , and CO, while positive but insignificant associations with SO 2 . The effects of PM 2.5 , PM 10 , NO 2 , O 3 , and CO were robust when adjusted for co-pollutants. We found stronger associations in the cool season than in the warm season. Moreover, there were non-significant differences in the associations between air pollution and sex or age group. This study suggests that patients with hypertension had an increased risk of hospital admission when exposed to air pollution., (Copyright © 2018 Elsevier Inc. All rights reserved.)

The continuous deterioration of the aquatic environment in rivers and streams is increasingly causing social and political tensions. To alleviate aquatic environmental problems, especially for the nonpoint source pollution, establishment of riparian forest buffers has been demonstrated as an effective control measure. However, few comprehensive studies of the reduction effects of riparian reforestation on the aquatic environment have been performed, particularly in identifying the suitable widths of reforestation projects. In this paper, the Annualized Agricultural Non-Point Source (AnnAGNPS) model was used to simulate the reduction effects of riparian reforestation on runoff and nutrient loads in Wucun watershed, China. The results showed that 20-m, 40-m, and 60-m widths of riparian buffer reforestation had significant effects on the yearly loads of total nitrogen (TN) and total phosphorus (TP), with reduced rates of 23.21 to 56.2% and 18.16 to 52.14%, respectively. The reduction effect on annual runoff varied from 2.8 to 5.4%. Furthermore, the reduction effect of nutrients performed best during the transition period, while the best runoff reduction was found during the dry period. These distinct reductions indicated that the implementation of riparian forest buffers was capable of reducing the risk and frequency of flooding and eutrophication, especially during the wet and transition periods. Additionally, the 20-m width of riparian buffer reforestation achieved the highest reduction efficiency for runoff, and the 40-m width was the most suitable reforested riparian buffer width for TN and TP. Therefore, 40 m may be the optimum buffer width for the implementation of riparian reforestation in the Wucun watershed. These research results provided scientific information on selecting the optimum buffer width for aquatic environmental regulators and managers as the reduction effects of different widths of riparian buffers on runoff and nutrients were different when considering buffer reforestation.

Objective: To characterize the prevalence and species/genotypes of Cryptosporidium spp. in farmed pigs in the north of the Yangtze River in Anhui Province., Methods: A total of 500 samples of pig feces were obtained from seven largescale pig farms in the north of the Yangtze River in Anhui Province. PCR and sequences analysis of the small subunit rDNA gene were used to detect and identify the Cryptosporidium species/genotypes., Results: The overall prevalence of Cryptosporidium was 4.8% (24/500). Additionally, Cryptosporidium prevalence was 40.0% in Qianshan and 6.3% in Chuzhou, respectively. No Cryptosporidium infection was found in other sampling areas. The DNA sequence analysis of the SSUrDNA gene revealed that all of the isolates represented C. scrofarum . The Cryptosporidium infection rate (9.1%) of pigs (> 60 days) was significantly higher than the rates of both pigs (< 30 days) and pigs (30-60 days) (both P < 0.01)., Conclusions: C. scrofarum in the farmed pigs in the north of the Yangtze River in Anhui Province may be a source of Cryptosporidium infection and pose a potential public health threat to humans and other animals, and therefore, the status should be paid more attention to.

Grasslands worldwide are suffering from overgrazing, which greatly alters plant community structure and ecosystem functioning. However, the general effects of grazing on community structure and ecosystem function at spatial and temporal scales has rarely been examined synchronously in the same grassland. Here, during 2011-2013, we investigated community structure (cover, height, and species richness) and aboveground biomass (AGB) using 250 paired field sites (grazed vs. fenced) across three vegetation types (meadow, typical, and desert steppes) on the Inner Mongolian Plateau. Grazing, vegetation type, and year all had significant effects on cover, height, species richness, and AGB, although the primary factor influencing variations in these variables was vegetation type. Spatially, grazing significantly reduced the measured variables in meadow and typical steppes, whereas no changes were observed in desert steppe. Temporally, both linear and quadratic relationships were detected between growing season precipitation and cover, height, richness, or AGB, although specific relationships varied among observation years and grazing treatments. In each vegetation type, the observed community properties were significantly correlated with each other, and the shape of the relationship was unaffected by grazing treatment. These findings indicate that vegetation type is the most important factor to be considered in grazing management for this semi-arid grassland.

Objective: To study the expression of a gene associated with retinoid-interferon (IFN)-induced mortality 19 (GRIM-19) in the villi of patients with missed abortion and possible pathogenesis., Design: Case-control study., Setting: University hospital., Patient(s): Villous samples were collected from a total of 50 women with missed abortion and 50 women with normal pregnancies., Intervention(s): Villous samples from the missed-abortion group were collected by curettage or manual vacuum aspiration. The control villous samples were obtained by vacuum aspiration., Main Outcome Measure(s): Protein and messenger RNA levels of GRIM-19 in villous samples were measured by Western blot and real-time polymerase chain reaction, and the location of GRIM-19 in trophoblasts was detected by immunohistochemistry. Apoptotic cells and microvessel density in villous sample were assayed by TUNEL and immunostaining. Mitochondrial membrane potential and apoptotic changes of the HTR-8/SVneo cell line in response to down-regulation of GRIM-19 were evaluated by 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide and annexin V/propidium iodide. Vascular endothelial growth factor production was measured by ELISA., Result(s): GRIM-19 protein and messenger RNA levels in the villi from women with missed abortion were found to be significantly lower than in women who had normal pregnancies. Many more apoptotic cells and lower microvessel density were detected in villi from patients with missed abortion. Knockdown of GRIM-19 in HTR-8/SVneo cells gave rise to a collapse of mitochondrial membrane potential and increase in apoptosis. Simultaneously, the vascular endothelial growth factor secretion in the HTR-8/SVneo cells culture medium decreased as GRIM-19 became down-regulated., Conclusion(s): The GRIM-19 deficiency in the villus may be associated with missed abortion via increasing apoptosis and affecting angiogenesis., (Copyright © 2015 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)

Background: A new histological classification by the International Association for the Study of Lung Cancer/American Thoracic Society/European Respiratory Society (IASLC/ATS/ERS) for lung adenocarcinoma (LAC) was proposed recently, in which a micropapillary pattern (MPP) was described., Objectives: This study aimed to evaluate the clinicopathological characteristics of LAC with MPP (LAC-MPP) and to investigate the correlation between LAC-MPP and epidermal growth factor receptor (EGFR) mutation status with the prognosis in Chinese patients., Patients and Methods: From May 2007 and February 2012, two hundred and forty-eight patients underwent resection and pathologically confirmed LAC. We classified all cases histologically according to the IASLC/ATS/ERS classification; an MPP ratio ≥5% was considered MPP-positive. Used Pearson's chi-square test to evaluate the relationships between EGFR mutation status and MPP status with patient clinicopathological characteristics., Results: There were MPP-positive tumors (MPP ratio ≥5%) in 31.9% of cases (79/248); the MPP ratio correlated with TNM stage (p=0.001) and lymph node metastasis (p=0.03). EGFR mutation (EGFR-mut) was detected in 87 cases (34.3%); 161 cases had wild-type EGFR (EGFR-wt). EGFR mutation was present in 65% of the MPP-positive subtype. Patients with EGFR-mut tumors had significantly longer overall survival (OS) (p=0.002). OS was also significantly longer in MPP-negative EGFR-mut or EGFR-wt patients (p<0.001)., Conclusion: These findings indicate that EGFR-mut tumors are likelier to be MPP-positive subtypes and that MPP may be a novel potential pathological marker of poor prognosis in Chinese LAC patients., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)

Pollution discharge disturbs the natural functions of water systems. The environmental microbial community composition and diversity are sensitive key indicators to the impact of water pollutant on the microbial ecology system over time. It is meaningful to develop a way to identify the microbial diversity related to heavy metal effects in evaluating river pollution. Water and sediment samples were collected from eight sections along the Tiaozi River where wastewater and sewage were discharged from Siping City in northeastern China. The main pollutants contents and microbial communities were analyzed. As the primary metal pollutants, zinc (Zn) and arsenic (As) were recorded at the maximum concentrations of 420 and 5.72 μg/L in the water, and 1704 and 1.92 mg/kg in the sediment, respectively. These pollutants posed a threat to the microbial community diversity as only a few species of bacteria and eukaryotes with strong resistance were detected through denaturing gradient gel electrophoresis (DGGE). Acinetobacter johnsonii, Clostridium cellulovorans, and Trichococcus pasteurii were the dominant bacteria in the severely polluted areas. The massive reproduction of Limnodrilus hoffmeisteri almost depleted the dissolved oxygen (DO) and resulted in the decline of the aerobic bacteria. It was noted that the pollution reduced the microbial diversity but the L. hoffmeisteri mass increased as the dominant community, which led to the overconsuming of DO and anaerobic stinking water bodies. Water quality, concentrations of heavy metals, and the spatial distribution of microbial populations have obvious consistencies, which mean that the heavy metals in the river pose a serious stress on the microorganisms.

Natural hybridization can lead to various evolutionary outcomes in plants, including hybrid speciation and interspecific gene transfer. It can also cause taxonomic problems, especially in plant genera containing multiple species. In this study, the hybrid status of Melastoma affine, the most widespread taxon in this genus, and introgression between its putative parental species, M. candidum and M. sanguineum, were assessed on two sites, Hainan and Guangdong, using 13 SSR markers and sequences of a chloroplast intergenic spacer. Bayesian-based STRUCTURE analysis detected two most likely distinct clusters for the three taxa, and 76.0% and 73.9% of the morphologically identified individuals of M. candidum and M. sanguineum were correctly assigned, respectively. 74.5% of the M. affine individuals had a membership coefficient to either parental species between 0.1 and 0.9, suggesting admixture between M. candidum and M. sanguineum. Furthermore, NewHybrids analysis suggested that most individuals of M. affine were F2 hybrids or backcross hybrids to M. candidum, and that there was extensive introgression between M. candidum and M. sanguineum. These SSR data thus provides convincing evidence for hybrid origin of M. affine and extensive introgression between M. candidum and M. sanguineum. Chloroplast DNA results were consistent with this conclusion. Much higher hybrid frequency on the more disturbed Guangdong site suggests that human disturbance might offer suitable habitats for the survival of hybrids, a hypothesis that is in need of further testing.

Background: Natural hybridization is prevalent in ferns, and plays an important role in fern evolution and speciation. In the Indo West-Pacific region, the mangrove fern genus Acrostichum consists of two largely sympatric species, A. aureum and A. speciosum. Although there has been no report of interspecific hybridization before, we found some individuals morphologically intermediate between them in Guangdong and Hainan, China, for the first time, which were suspected to be hybrids. In this study, we aimed to test the hypothesis of natural hybridization between A. aureum and A. speciosum in Guangdong and Hainan using three low-copy nuclear genes. A chloroplast intergenic spacer was used to infer the hybridization direction once the hybrid status was confirmed. In addition, we examined spore shapes and germination for these taxa., Results: Both A. aureum and A. speciosum showed a low level of polymorphism at all three nuclear genes; however, they were well separated at these loci. At both locations, each individual of the putative hybrid showed additivity in chromatograms at all sites where the two species showed fixed differences. Haplotype analysis at all three nuclear genes indicated that each individual of the putative hybrid possessed two haplotypes, matching with those of A. aureum and A. speciosum, respectively. Sequencing of the chloroplast trnV-trnM regions showed that A. aureum differed from A. speciosum by eleven nucleotide substitutions and three indels (insertions/deletions), and all sampled individuals of the putative hybrid had the identical sequences with A. speciosum. Compared with A. aureum and A. speciosum, the putative hybrid had much reduced spore germination rate., Conclusions: Sequence data of the three nuclear genes provide compelling evidence for natural hybridization between A. aureum and A. speciosum, and all the hybrid individuals are likely F1s. The hybridization is unidirectional and A. speciosum is the maternal parent of the hybrid based on the assumption of maternal inheritance of chloroplast DNA. Human disturbance on mangrove habitats may facilitate the establishment of hybrids of Acrostichum.

This research firstly reported the molecular analysis of ECHO25 (Entric Cytopathic Human Orphanviruses Type 25). To clarify molecular characteristics of the ECHO25 virus isolates in Henan Province and its relationship with the rest of world's isolates,the complete VP1 sequences of the 4 isolates in Henan were successfully amplified by RT-PCR and were compared with other ECHO25 isolates available from GenBank. Compared with the prototype strain JV-4, the nucleotide sequence identity was 79.2%-80.1%, and the amino sequence identity was 89.0%-92.4, the nucleotide sequence identity among the 4 strains isolates in Henan Province was 93.0%-99.0%, the amino sequence identity was 92.4%-97.5%. HN-01 and HN-26 strains had the highest level of homology, the nucleotide homology was 99.0%; All the 4 strains belonged to the B1 genotype.

In this study, the single extraction and sequential extraction procedure were used to separate Pb from an old smelter soil in Liaoning Province, China. At the same time, the root and the overground parts of six species plants in the smelter were harvested for analysis. The concentrations of Pb in the roots and the overground parts of the plants were then compared with the amounts of Pb extracted from the rhizosphere soil by range of conventional extractants. The speciation distribution of Pb was RES > OX > OM > WSA > SE. Assuming that metal mobility and bioavailability are related to their solubility and the contents in typical plants in the contaminated soil, Pb would be the potential risk to environment safety in the area.

Based on the investigation of heavy metal pollutions on an abandoned metal smelter site in Shenyang, and by using the United States Environmental Protection Agency methodologies for human health risk assessment, the human health risk of the polluted soil on the study site was assessed. For the defined future land use patterns, the industrial (I) and recreational (II) exposure scenarios were assumed and evaluated. The combined hazard index for all the contaminants of potential concern was 2.65 x 10(-2) for Scenario I and 3.67 x 10(-2) for Scenario II. Regarding the potential carcinogenic risk associated with inhalation route, only cadmium was a contributor, with the risk of 4.48 x 10(-9) and 7.30 x 10(-10) for Scenario I and Scenario II, respectively. The hazard indices were less than a unit, and the carcinogenic health risk was negligible. The potential health risks of the study site (for both industrial and recreational scenarios) were mainly associated with the exposure to copper. The risk-based remedial goals calculated for the industrial scenario were lower than the environment quality risk assessment criteria for soil at the manufacturing facilities of China.

In order to evaluate ecological risk of agrochemicals in agricultural environment, single and joint toxic effects of an important herbicide and a typical heavy metal on root elongation of crops were investigated. Seeds of the three crops including wheat (Triticum aestivum), Chinese cabbage (Brassica pekimensis) and soybean (Glycine max) as the main crops in northeast China were exposed to acetochlor as a herbicide and lead (Pb) as a heavy metal using the pot-culture method, and meadow brown soil as one of the main soils distributed in northeast China was applied in the investigation. The results indicated that the interactive effects of the two pollutants on root elongation of the three crops were very complicated although they had markedly significant (P < 0.01) linear interrelationships based on the regression analyses. When the concentration of added Pb2+ reached 200 mg/kg, acetochlor and Pb had an antagonistic effect on the inhibition of root elongation of the three crops. However, acetochlor and Pb had significantly (P < 0.05) synergic effects on the inhibition of root elongation when concentration of added Pb2+ was up to 1000 mg/kg. At the low concentration of added Pb, joint toxicity of acetochlor and Pb was more dependent on the concentration of Pb. Among the three crops, wheat was the most sensitive to the toxicity of Pb and Chinese cabbage was the most sensitive to the toxicity of acetochlor.

Groundwater quality with respect to nitrate, major inorganic constituents, stable isotopes, and tritium was assessed in the agricultural Tangshan region in the Hai He River Basin of the People's Republic of China and compared with three regions in the U.S.: the Delmarva Peninsula of Delaware, Maryland, and Virginia; the San Joaquin Valley of California; and the Sacramento Valley of California. The China and U.S. regions are similar in size and land use, but have different climatic conditions and patterns of water use for irrigation. The Tangshan region has been in agricultural production for a much longer time, probably several centuries, than the three U.S. regions; however, the widespread use of synthetic fertilizers and other soil amendments probably started at a similar time in all four regions. In all four regions, median nitrate concentrations were generally below the U.S. drinking water standard of 10 mg/l of nitrate as nitrogen. However, higher concentrations and a greater range were evident for the Tangshan region. In the water samples collected from a shallow aquifer in the Tangshan region (over 25% of all samples), nitrate concentrations exceeded the Chinese standard of 20 mg/l, whereas few comparative samples (2.6%) collected in the U.S. exceeded 20 mg/l. In Tangshan, relatively low nitrate, which is indicative of uncontaminated background concentrations, was measured in older water of deeper wells. Recently recharged water was detected in wells drilled as deep as 150 m. Nitrate concentrations above background levels were also measured in water samples from these wells. In addition to nitrate, the agricultural area of the Tangshan region has been affected by elevated total dissolved solids and iron, the latter attributed to widespread application of animal wastes and sewage deposited on the land surface, which lead to oxygen depletion in the subsurface environment and dissolution of iron. The elevated total dissolved solids of the Tangshan study area could not be attributed to any one process.

75 Chinese and 75 American students' perceptual styles of cognitive complexity and scanning (focus versus nonfocus) were analyzed. The American students, 22 Chinese women, and 18 Chinese social science and humanities students scored as more complex than the 53 Chinese men and 57 Chinese natural science and engineering students. It was also observed that the Chinese men and American women scored as more focused than the 22 Chinese women and 43 American men.

The Group Embedded Figures Test of Oltman, Raskin, and Witkin was administered to 75 Chinese and 75 American students at an American university to measure their perceptual styles of field independence versus field dependence. The analysis showed that these students had a similar style of field independence.

The underlying DNA changes associated with glucose-6-phosphate dehydrogenase (G6PD)-deficient Asians have not been extensively investigated. To fill this gap, we sequenced the G6PD gene of 43 G6PD-deficient Chinese whose G6PD was well characterized biochemically. DNA samples were obtained from peripheral blood of these individuals for sequencing using a direct polymerase chain reaction (PCR) sequencing procedure. From these 43 samples, we have identified five different types of nucleotide substitutions in the G6PD gene: at cDNA 1388 from G to A (Arg to His); at cDNA 1376 from G to T (Arg to Leu); at cDNA 1024 from C to T (Leu to Phe); at cDNA 392 from G to T (Gly to Val); at cDNA 95 from A to G (His to Arg). These five nucleotide substitutions account for over 83% of our 43 G6PD-deficient samples and these substitutions have not been reported in non-Asians. The substitutions found at cDNA 392 and cDNA 1024 are new findings. The substitutions at cDNA 1376 and 1388 account for over 50% of the 43 samples examined indicating a high prevalence of these two alleles among G6PD-deficient Chinese. Our findings add support to the notion that diverse point mutations may account largely for much of the phenotypic heterogeneity of G6PD deficiency.

Using a direct PCR sequencing technique, we have identified two DNA base substitutions in 8 different biochemical G6PD variants of Chinese origin. Neither one of these abnormalities has been reported in other ethnic groups. An abnormality (C1) of G to T substitution at cDNA 1376 causing an amino acid change from Arg to Leu has been found in 3 variants. Another abnormality (C2) of G to A substitution at cDNA 1388 causing an amino acid change from Arg to His has been found in 5 variants. Both C1 and C2 are located in exon 12 of the G6PD gene and are only 12 base pairs apart. However, C1 is associated with a significant increase in the deamino-NADP utilization rate, whereas C2 is not. Taken together, our data suggest that C1 and C2 are very common among Chinese with a G6PD deficiency and exon 12 may define an important functional domain of the human G6PD.