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Abstract: We have studied the chromosomes of lymphocytes of the residents in a high background radiation area (HBRA) and a control area (CA) in the southern China. The level of natural radiation in HBRA is three to five times higher than that in control area. Unstable type aberrations (dicentrics and rings) were analyzed in 22 individuals in HBRA and 17 individuals in CA. Stable type aberrations (translocations) were examined in 28 elderly individuals and 6 children in HBRA and 24 elderly individuals and 8 children in CA. There was statistically significant difference in the increasing rates between those two areas in adult. In case of stable aberrations, the frequencies were much higher than those of the unstable aberrations both in HBRA and in control. Statistically, there was no difference in the frequencies between HBRA and CA, but the frequencies in children were lower than those in elderly individuals in both groups. Individual variation was small in children while that in adults was large. The amount of chromosome aberrations induced by radiation is not significant when it is compared with that induced by other mutagenic factors such as smoking in the Southern China. [Copyright &y& Elsevier]

The Soil Moisture Experiment in the Luan River (SMELR) was conducted from 2017 to 2018 in the semi-arid Luan River watershed located in the North of China. One of the objectives of SMELR is to serve as an assessment tool and demonstration for a new Terrestrial Water Resources Satellite (TWRS) concept with one-dimensional synthetic aperture microwave techniques, for which soil moisture retrieval under variable satellite observing configurations (mainly in terms of incidence angels) is the greatest challenge. This proposed mission is targeted to provide continuity for the current satellite L-band microwave observations, and further improve the accuracy and spatial resolution of soil moisture mapping through the synergistic use of active, passive and optical remote sensing data. Multi-resolution, multi-angle and multi-spectral airborne data were obtained four times over a 70 km by 12 km area in the Shandian River basin, and one time over a 165 km by 5 km area that includes the Xiaoluan River basin. The near surface soil moisture (0 cm–5 cm) was measured extensively on the ground in fifty 1 km by 2 km quadrats (targeted to compare with the airborne radiometer), and two hundred and fifty 200 m by 200 m quadrats corresponding to radar observations. Two networks were established for continuous measurement of the soil moisture and temperature profile (3 cm, 5 cm, 10 cm, 20 cm, 50 cm) and precipitation in the Shandian and Xiaoluan River basin, respectively. Supporting ground measurements also included ground temperature, vegetation water content, surface roughness, continuous measurement of microwave emission and backscatter at a pasture site, reflectance of various land cover types, evapotranspiration and aerosol observations. Preliminary results within the experimental area indicate that (1) the near surface soil moisture spatial variability at a 200 m scale was up to ~0.1 cm3/cm3 at an intermediate value of ~0.35 cm3/cm3. (2) The difference of soil and vegetation temperature in grass and croplands reach its maximum of 11 K around solar noon time, and the soil temperature gradient is largest at around 15 P.M. (3) Both the airborne and ground measurements cover a wide range of conditions. The L-band active and passive observations exhibit a large variation of ~30 dB and ~80 K, respectively, corresponding to soil moisture range from 0.1 cm3/cm3 to 0.5 cm3/cm3. The sensitivity of both active and passive data to soil moisture is compared at corresponding spatial resolutions and show high information complementarity for better accuracy and resolution soil moisture retrieval. • First airborne optical and active-passive microwave experiment conducted in China • Demonstration of the active-passive microwave synergy at varying incidence angles • Addressing prelaunch science questions of the terrestrial water resources mission [ABSTRACT FROM AUTHOR]

Unbalanced and degraded mixtures (UDM) are frequently encountered during forensic DNA analysis. For example, forensic DNA units regularly encounter DNA mixture signal where the DNA signal from the alleged offender is masked or swamped by high quantities of DNA from the victim. Our previous data presented a new kind of DNA markers that composed of a deletion/insertion polymorphism (DIP) and a SNP and we termed this new kind of microhaplotypes DIP-SNP (combination of DIP and SNP). Since such markers could be designed short enough for degraded DNA amplification, we hypothesized that DIP-SNP markers are applicable for typing of UDM. In this study, we developed a new set of DIP-SNPs with short amplicons which were complement to our prior developed system. The multiplex PCR and SNaPshot assay were established for 20 DIP-SNPs in a Chinese Han population. The DIP-SNPs were capable of detecting the minor contributor's allele in home-made DNA mixture with sensitivities from 1:100 to 1:1000 with a total of 1 -10 ng input DNA. Moreover, this system successfully typed the degraded DNA whether it came from the single source or mixture samples. In Chinese population, the system showed an average informative value of 0.293 and combined informative value of 0.998363862. Our results demonstrated that DIP-SNPs may serve as a valuable tool in detection of UDM in forensic medicine., (© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Unbalanced DNA mixture is still a difficult problem for forensic practice. DIP-STRs are useful markers for detection of minor DNA but they are not widespread in the human genome and having long amplicons. In this study, we proposed a novel type of genetic marker, termed DIP-SNP. DIP-SNP refers to the combination of INDEL and SNP in less than 300bp length of human genome. The multiplex PCR and SNaPshot assay were established for 14 DIP-SNP markers in a Chinese Han population from Shanxi, China. This novel compound marker allows detection of the minor DNA contributor with sensitivity from 1:50 to 1:1000 in a DNA mixture of any gender with 1 ng-10 ng DNA template. Most of the DIP-SNP markers had a relatively high probability of informative alleles with an average I value of 0.33. In all, we proposed DIP-SNP as a novel kind of genetic marker for detection of minor contributor from unbalanced DNA mixture and established the detection method by associating the multiplex PCR and SNaPshot assay. DIP-SNP polymorphisms are promising markers for forensic or clinical mixture examination because they are shorter, widespread and higher sensitive., (Copyright © 2018 Elsevier Inc. All rights reserved.)

Cytogenetic investigation of stable-type aberrations (translocations) was carried out with our improved methods on 28 elderly individuals in a high-background radiation area (HBRA) in China, and on 24 elderly individuals in a control area (CA). The level of radiation in HBRA is 3 to 5 times higher than in CA. The mean frequencies of translocations per 1,000 cells in HBRA and CA were 12.4 +/- 5.3 and 10.0 +/- 3.8, respectively. No significant difference was found in the frequencies between HBRA and CA (P>0.05, Mann-Whitney U test). When elderly individuals in HBRA and CA were classified into four subgroups of HBRA nonsmokers, HBRA smokers, CA nonsmokers, and CA smokers, a significant difference was found in the frequencies between CA smokers and CA nonsmokers (P<0.05, Mann-Whitney U test). Furthermore a tendency of difference (a near T-value of 0.05 level) was found in a comparison of HBRA smokers vs. CA nonsmokers. The present results indicate that the elevated level of natural radiation in HBRA plays a less significant part than smoking in bringing about the induction rate of stable-type aberrations (translocations) in those areas.

Cytogenetic investigation of stable type aberrations (translocations) was performed with our improved methods in 6 children and 15 elderly persons in a high background radiation area (HBRA) in China, and in 8 children and 11 elderly persons in a control area. The total numbers of cells analyzed in elderly persons were 68,297 in HBRA and 35,378 in controls and in children were 45,535 in HBRA and 56,198 in controls. On average 5138 cells per subject were analyzed. The variation in the frequencies of translocations per 1000 cells was small in children while it was large in elderly persons. No significant difference was found in the frequencies between HBRA and control (P > 0.05, Mann-Whitney U test). On the other hand, correlation between age and translocation frequencies was significant at the 1% level (rs = 0.658 with 37DF, Spearman rank correlation test). The contribution of an elevated level of natural radiation in HBRA in China to the induction of stable type chromosome aberrations does not have a significant effect compared with the contribution of chemical mutagens and/or metabolic factors. The present study suggests that the probability of the risk of causing malignant and/or congenital diseases by the increased amount of radiation is imperceptible in HBRA where the level of natural radiation is 3 to 5 times higher than that in the control area.