1. Genetic diversity of Lepista nuda (Agaricales, Basidiomycota) in Northeast China as indicated by SRAP and ISSR markers.
- Author
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Du J, Guo HB, Li Q, Forsythe A, Chen XH, and Yu XD
- Subjects
- Agaricales classification, Agaricales isolation & purification, Antioxidants chemistry, China, Climate, Cluster Analysis, DNA, Fungal genetics, DNA, Fungal metabolism, Phylogeny, Polymorphism, Genetic, Agaricales genetics, Genetic Variation, Microsatellite Repeats genetics, Nucleic Acid Amplification Techniques methods
- Abstract
Lepista nuda is a popular wild edible mushroom that grows in China. In this study, we used ISSR and SRAP molecular markers to analyze the genetic diversity of 72 samples of L. nuda from eight populations in Northeast China. In total, six ISSR primers and five pairs of SRAP primers that produced clear and polymorphic banding profiles were selected for assessing L. nuda genetic diversity. The results revealed a high level of genetic variation among the 72 samples (94.4% polymorphism) but a low degree of gene flow among the populations. Among L. nuda populations, genetic distance was not correlated significantly with geographic distance. The antioxidant activity of the samples from each population was also tested and the result showed that all the selected samples had more than 60% DPPH scavenging activities. Nonetheless, the antioxidant activity diversity is not coincident with both the genetic diversity and the geographic distribution. The results indicate that ISSR and SRAP molecular markers are useful for studying the genetic diversity of L. nuda. The results also suggest that L. nuda populations in Northeast China require protection., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2018
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