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Information and communications technology (ICT) is now widely used in education. However, the effect of ICT on the development of students' scientific higher-order thinking skills (S-HOTS) is a controversial issue in science education research. This has attracted significant attention from researchers worldwide in the field of educational technology. Purpose: In this study, a novel framework for students' S-HOTS is proposed based on Marzano's new taxonomy to determine the impact of ICT on S-HOTS. And exploring the difference in the impact of ICT on S-HOTS in two different countries. Sample: The data for this study were obtained from the Science Assessment and ICT Familiarity Questionnaire of the PISA 2015 survey for Finnish and Chinese students. This survey measures the differences in economic and educational levels and literacy in each country and tests the knowledge and skills necessary for life in reading, mathematics, and science for students (15 years old), through a detailed pre-testing country field study to make the results comparable across countries. Design and methods: We used the Delphi methodology to determine the framework of S-HOTS and a regression analysis to analyse the effects of ICT on students' S-HOTS. Results: The results demonstrate how the availability, use frequency and use time of ICT affect S-HOTS. Furthermore, we compared the results between China (9841 students) and Finland (2538 students), and these findings demonstrated that ICT use at school has a negative effect on S-HOTS, especially ICT use time in school. We also found that using an interactive whiteboard positively and significantly affects S-HOTS. Conclusion: The results suggest that the effect of ICT use on S-HOTS in school depends on the ICT method combined with the learning process. Consequently, the use of ICT in schools should be viewed dialectically to promote students' S-HOTS.

A thorough insight into the initiation, segmentation, propagation and interaction of multitrend basin-bounding faults is crucial to restoring the growth history of the faults and clarifying the fault growth pattern and its influence on the structures developed along the margin due to the growth of the basinbounding faults, but systematic studies on the individual influence of the evolution of each fault segment on the present structure are lacking. Based on 3D seismic data, the timing and growth of multitrend basin-bounding faults were analysed using T-z plots and throw backstripping, allowing us to determine the individual effects that each fault segment evolution exerteds on the present-day configuration of the northern margin of the Nanpu Sag. The basinbounding fault is composed of the Xinanzhuang and Baigezhuang faults, and the Xinanzhuang fault comprises three linked segments with varying orientations (i.e., NE-SW, E-W, and NNE-SSW). In comparison, the Baigezhuang fault comprises only two linked NW-SE-oriented fault segments. The evolution process can be divided into three stages. (1) During the early synrift I stage, namely, the isolated fault stage, five isolated multitrend basin-bounding segments were active. (2) During the late synrift I stage, namely, the hard-linkage stage, the five segments propagated laterally and linked with each other, behaving as a single fault. Meanwhile, the NE-trending No. 5 Fault bifurcated upward from the basin-bounding fault to accommodate local stress, and the NW-trending Gaobei Fault deviated from the basin-bounding fault controlled by local stresses induced by differential activities of the multitrend fault segments under the same far-field stress. (3) During the synrift II to postrift linkage development stage, the extension orientation changed from NW-SE- to N-S, and additional displacement accumulated along the basin-bounding fault without further lateral propagation. Newly formed E-W- trending faults developed orthogonal to the extension orientation and linked with preexisting NE- or NW- trending faults, forming a complex fault zone. In addition, influenced by the geometry of the basin-bounding fault, the Laoyemiao Anticline formed by gravitational collapse under the dual action of a rollover anticline and transverse anticline. Furthermore, the evolution of the basin-bounding faults played an important role in controlling the source-to-sink system, and the transition zone was the main provenance channel formed by the segmented growth of the faults. This study provides new insight into multitrend large fault evolution, and their impact on basin development provides a comprehensive explanation of the later structures developed in polyphase rifts. [ABSTRACT FROM AUTHOR]

Soil nitrogen (N) availability is critical to root function and morphological traits, which reflect strategies for nutrient uptake and survival of plants. However, these factors have rarely been studied in temperate old-growth forests, especially understory shrubs. Therefore, we conducted an 8-year continuous N addition field experiment in Lesser Khingan Mountains northeastern China, including four N treatments. The results showed that under low and medium N treatments, the root diameter of all shrubs generally increased, which was caused by the increase in root stele diameter and cortical thickness, which were mainly regulated by soil NH4+-N. Low N treatment significantly increased the ratio of stele to root diameter, promoted the development of stele and tracheid characteristics, and thus improved the transport capacity of fine roots. High N treatment decreased the cortical thickness of all shrubs, but increased the total area and number of tracheid, which might have a significant positive impact on the growth of fine roots. These results suggest that N deposition may affect the physiological function and activity of fine roots by affecting their diameter, cortical thickness, and stele diameter. Collectively, plants can adjust their root nutrient absorption strategies to adapt to changes in the nutrient environment. [ABSTRACT FROM AUTHOR]

Cinnamomum camphora (C. camphora) is a broad-leaved evergreen tree cultivated in subtropical China. Currently, the use of C. camphora clonal cuttings for coppice management has become popular. However, the effects of C. camphora coppice planting on soil abiotic and biotic variances remained unclear. In this study, we collected soil from three points in the seven-year C. camphora coppice planting land: under the tree canopy (P15), between trees (P50), and abandoned land (Control) to investigate the effects of C. camphora coppice planting on soil fertility, microbial community structure and enzyme activity. The results revealed that C. camphora coppice planting significantly increased soil fertility in the point under the tree canopy (P15) and point between trees (P50), and P15 had more significant effects than P50. Meanwhile, in P15 and P50, soil bacterial, fungal alpha-diversity were improved and microbial community structures were also changed. And the changes of soil organic carbon and total nitrogen promote the transformation of soil bacterial, fungal community structures, respectively. In addition, C. camphora coppice planting significantly (p < 0.05) increased soil urease (UE), polyphenol oxidase, and peroxidase activities, while significantly decreased soil ACP activity. This study demonstrated that the C. camphora coppice planting could improve soil fertility in subtropical China, which promoted the transformation of soil microbial community from oligotrophs (K-strategist) to copiotrophs (r-strategist). Thus, this work can provide a theoretical basis for soil nutrient variation and productive management of C. camphora coppice plantation in subtropical China. [ABSTRACT FROM AUTHOR]

Background: Allergic diseases are highly prevalent in the women of childbearing age. As we know, the immune system could change when pregnancy, which may affect the course of allergic diseases. Meanwhile, they also can affect the course and outcome of pregnancy. The data on incidence of allergies during pregnancy is lacking and conducting clinical trials in pregnant women was limited, therefore, we observed a prebirth cohort to supplement the relevant data and strengthen concerned research conductions. Objective: We aim to obtain the incidence of allergies in urban pregnancy and explore the relevant factors of allergic diseases in urban pregnancy. Methods: We design a multicenter and prospective cohort in 20 institutions above municipal level which were eligible according to the study design from 14 provinces covering all-side of China. This cohort was conducted from 13+6 weeks of gestation to 12 months postpartum and in our study, we chose the prenatal part to analyze. The outcome was developing allergies during pregnancy, which were diagnosed by clinicians according to the uniform criterion from National Health Commission. All the data was collected by electronic questionnaires through tablet computers. Results: The incidence of allergic diseases in urban pregnant women was 21.0% (95%CI 20.0% ~ 22.0%). From social demography data, the history of allergies of pregnant women and their parents had statistical significance(p < 0.01); For exposure to living or working environment, house decoration for less than half a year, exposure to plush toys, disinfectants, insecticides, antihistamines, glucocorticoids, antipyretic analgesics, tocolytic agent and probiotics had statistical significance (all p < 0.05); For psychological status, self-rated depression and anxiety had statistical significance (p = 0.026;p = 0.006). Conclusion: The incidence of allergic diseases in urban pregnant women was similar to the former study and kept a medium–high level. The history of allergies of pregnant women and their parents, house decoration time, exposure to plush toys, disinfectants, insecticides, antihistamines, glucocorticoids, antipyretic analgesics, tocolytic agents, probiotics, self-rated depression, and anxiety were relevant factors of allergic diseases during pregnancy. [ABSTRACT FROM AUTHOR]

Background: Helicobacter pylori (H. pylori) infection is one of the most common chronic bacterial infections worldwide. The resistance of H. pylori to antibiotics may increase the risk of treatment failure. Complementary and alternative regimens are still needed. This study aimed to critically assess the efficacy and safety of Jinghua Weikang capsule (JWC) for H. pylori eradication. Materials and methods: PubMed, Embase, Web of Science, Cochrane library, China National Knowledge Infrastructure, Wanfang Digital Periodicals, and Chinese Science and Technology Periodicals database were searched from inception to April 2022. Randomized controlled trials (RCTs) comparing a combination of JWC and conventional treatments with conventional treatments alone or combined with a placebo for H. pylori eradication were considered for inclusion. The primary outcome was H. pylori eradication rate. The meta-analysis and trial sequential analysis (TSA) were conducted where possible. Results: A total of 34 studies were included in the statistical analysis. A pooled result showed that JWC with the duration of 2 weeks combined with the triple/quadruple therapy could significantly increase the H. pylori eradication rate compared with the triple/quadruple therapy alone (RR: 1.13, 95% CI: 1.05 to 1.21, p = 0.0008). However, the evidence of benefit was not confirmed by TSA. Another pooled result showed that JWC with the duration of 4 weeks combined with the triple/quadruple therapy could significantly increase the H. pylori eradication rate compared with the triple/quadruple therapy alone (RR: 1.21, 95% CI: 1.15 to 1.27, p < 0.00001). The evidence of benefit was confirmed by TSA. There were no statistically significant differences in the incidence of adverse reactions between the two groups. Conclusion: The present study suggests that JWC with the duration of 4 weeks can significantly improve the H. pylori eradication rate and should be considered as a complementary treatment to conventional regimens for H. pylori eradication. However, more high-quality RCTs are still needed to confirm these findings. [ABSTRACT FROM AUTHOR]

Background: Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods: In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), 109 CFU/mL inactivated B. abortus A19 strain (I group), 105 CFU/mL (low-dose group, L group) 106 CFU/mL live B. abortus A19 strain (high-dose group, H group), or 105 CFU/mL live B. abortus A19 strain combined with 109 CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results: The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of CD4+ and CD8+ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, CD4+ and CD8+ T cells in these mice were similar to those of the mice in the H group. Conclusions: Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine. [ABSTRACT FROM AUTHOR]

Brucella abortus is an important zoonotic pathogen that causes severe economic loss to husbandry and poses a threat to human health. The B. abortus A19 live vaccine has been extensively used to prevent bovine brucellosis in China. However, it is difficult to distinguish the serological response induced by A19 from that induced by natural infection. In this study, a novel genetically marked vaccine, A19ΔvirB12, was generated and evaluated. The results indicated that A19ΔvirB12 was able to provide effective protection against B. abortus 2308 (S2308) challenge in mice. Furthermore, the safety and protective efficacy of A19ΔvirB12 have been confirmed in natural host cattle. Additionally, the VirB12 protein allowed for serological differentiation between the S2308 challenge/natural infection and A19ΔvirB12 vaccination. However, previous studies have found that the accuracy of the serological detection based on VirB12 needs to be improved. Therefore, we attempted to identify potential supplementary antigens with differential diagnostic functions by combining label-free quantitative proteomics and protein chip technology. Twenty-six proteins identified only in S2308 were screened; among them, five proteins were considered as potential supplementary antigens. Thus, the accuracy of the differential diagnosis between A19ΔvirB12 immunization and field infection may be improved through multi-antigen detection. In addition, we explored the possible attenuation factors of Brucella vaccine strain. Nine virulence factors were downregulated in A19ΔvirB12. The downregulation pathways of A19ΔvirB12 were significantly enriched in quorum sensing, ATP-binding cassette transporter, and metabolism. Several proteins related to cell division were significantly downregulated, while some proteins involved in transcription were upregulated in S2308. In conclusion, our results contribute to the control and eradication of brucellosis and provide insights into the mechanisms underlying the attenuation of A19ΔvirB12. [ABSTRACT FROM AUTHOR]

We present comprehensive petrological, major-trace element, in situ zircon U-Pb dating and Sr-Nd-Pb isotopic data for Muchen granitoid (western Zhejiang Province, Southeast China), to constrain the petrogenesis of alkaline A-type granites and the geodynamic setting of Southeast China in the Early Cretaceous. The Early Cretaceous Muchen quartz monzonite yielded zircon U-Pb crystallization ages of 111.3 ± 0.7 Ma and is metaluminous to weakly peraluminous with SiO2 contents ranging from 59 to 69 wt.%, and can be classified as alkaline A-type granitoid. The quartz monzonites have low (87Sr/ 86Sr)i values (0.7052 to 0.7061) and high εNd(t) values (-2.6 to -2.0), similar to nearby coeval mafic rocks that have been proposed to be derived from the enriched lithospheric mantle. The high Nb/Ta ratios (16.7 to 30.1, average 21.8) and low Nb/U ratios (as low as 3.5) indicate the involvement of slab-derived melt and fluids in this mantle. These geochemical properties of the Muchen quartz monzonites indicated that they might be from a phlogopite-bearing and rutile-rich subduction-modified subcontinental lithospheric mantle, and underwent strong fractional crystallization of olivine + orthopyroxene + plagioclase during magma ascent. The low Mg# values of these alkaline rocks (<30 mostly) may indicate a low-pressure source in a back-arc setting. The early Cretaceous alkaline granitoids in Southeast China are related to the continental back-arc setting caused by deep angle subduction of the paleo-Pacific plate. [ABSTRACT FROM AUTHOR]

Subduction is a fundamental geodynamic process that transfers carbon from Earth's surface into the mantle. However, current understanding of the migration mechanisms, final storage region, and species involved in carbon recycling from continental crust remains limited. Here, we investigated the compositions of polyphasic inclusions and Mg isotopes in postcollisional mafic magmatic rocks from the Dabie Shan region of China. The main rock-forming minerals contained two distinct types of polyphasic inclusions, which displayed systematic differences in daughter mineral/gaseous phase assemblages, including host-like silicates ± carbonates (magnesite, dolomite, and calcite) + CH4 and carbonates + talc ± SiO2 (aqueous) + CH4, respectively. These inclusions indicate that carbon-rich silicate melts and carbon-rich magmatic fluids were trapped by host minerals during magmatic processes. The abundant carbonates and CH4 in both types of inclusions suggest that the mantle source of these postcollisional mafic magmatic rocks was rich in carbon, most likely existing in the forms of CO2 and CH4. Moreover, the studied postcollisional mafic magmatic rocks have mantle-like Mg isotope compositions, with d26Mg values ranging from -0.23‰ to -0.16‰. The combined observations of polyphase inclusions and Mg isotopes indicate that a substantial carbon-rich mantle domain arose from the metasomatism of silicate melts derived from subducted continental slabs that had dissolved a certain quantity of CO2 and CH4. We proposed that continental subduction is an efficient pathway for transporting crustal carbon into an orogenic subcontinental lithospheric mantle wedge, where the recycled carbon can be stored for >100 m.y. and eventually released to the surface during postcollisional magmatism. [ABSTRACT FROM AUTHOR]

Rotavirus B (RVB) is a causative agent leading to acute viral gastroenteritis diarrhea in both children and young animals, and has been commonly detected in piglets. In order to determine the causative agent of diarrheal outbreak occurring in December 2022 in piglets from a pig herd in Luoyang, Henan province of China, four common viral pathogens causing piglet diarrhea-three coronaviruses and rotavirus A (RVA) were first tested and found negative, therefore metagenomic sequencing was performed to explore other potential pathogens in the diarrheal samples. Unexpectedly, the most abundant viral reads mapped to RVB, and were de novo assembled to complete 11 viral gene segments. Sequence comparisons revealed that 5 gene segments encoding VP1, VP2, VP3, NSP3 and NSP4 of RVB strain designated as HNLY-2022 are most closely related to RVB strains derived from herbivores with low nucleotide similarities of 65.7–75.3%, and the remaining segments were relatively close to porcine RVB strains with the VP4 gene segment showing very low nucleotide identity (65.0%) with reference strains, indicating HNLY-2022 is a new reassortant RVB strain. Based on the previously proposed genotype classification criterion, the genotype constellation of RVB strain HNLY-2022 is G6-P[6]-I4-R6-C6-M6-A7-N5-T7-E5-H4 with more than half of the genotypes (P[6], R6, C6, M6, T7 and E5) newly reported. Therefore, the new reassortant RVB strain is the likely causative agent for the diarrheal outbreak of piglets occurred in China and more epidemiological studies should be conducted to monitor the spread of this newly identified porcine RVB strain. • Outbreak of piglet diarrhea occurred in Henan province of China was associated with a new rotavirus B. • HNLY-2022 is a reassortant RVB strain with the gene segments probably arose from pigs and herbivores. • The genotypes P[6], R6, C6, M6, T7 and E5 of RVB were firstly identified. [ABSTRACT FROM AUTHOR]

• Four current and one earlier CSFV field isolates in China were classified as either highly or moderately virulent strains. • E2 and Erns antibodies in infected pigs were generally inadequate for protection, or even absent in some infected pigs. • Viremia and Erns protein were present and long-lasting in the blood of all infected animals. Classical swine fever (CSF) remains an important pig disease in China, where it usually presents with mild or atypical clinical manifestations, with large scale outbreaks rarely seen. This has led to speculation about the possible circulation of viral strains of low virulence. To investigate this possibility, five field isolates within the predominant genotype 2 (2.1b, 2.1c, 2.1 h and 2.2) were evaluated and compared by experimental infection of naturally farrowed but colostrum-deprived piglets. All infected piglets displayed clinical signs, including persistent high fever, depression, anorexia, dyspnea, conjunctivitis, constipation, and hesitant gait. Typical pathological lesions, including pulmonary edema, hemorrhagic or cellulosic exudation, and swelling and hemorrhage of lymph nodes, were observed. Viremia and Erns protein expression in the blood of all infected animals were detectable from 3 to 5 days post infection (DPI), their presence correlating with the onset of fever, clinical signs and leukopenia. E2 antibody did not develop in any of the field CSFV-infected piglets during the disease course, while Erns antibody was detectable in 4–56% of infected animals at various time points. Mortalities ranged from 20 to 80% within 21 DPI, progressing to 100% by 43 DPI. Based on clinical scores and fatalities within 21 DPI, 2 of the 5 field isolates were classified as of moderate virulence and 3 of high virulence; i.e. , no field isolates of low virulence were identified. The study has provided data supporting the use of these isolates as challenge viruses to evaluate the efficacy of current CSF vaccines. [ABSTRACT FROM AUTHOR]

Zircon SIMS U-Pb dating of the Poshi, Hongshishan, Bijiashan, and Huangshan Ni-Cu-bearing and Xiangshan Ni-Cu-Ti-Fe-bearing mafic-ultramafic intrusions in the Eastern Tianshan and Beishan Rift yields a relatively restricted range of 278.6 Ma to 284.0 Ma. The histogram of compiled age data of basalts in the Tarim Basin and mafic-ultramafic intrusions in the Eastern Tianshan and Beishan Rift has a peak of 280 Ma, which probably represents the time of mantle plume activity. The basalts have lower εNd(t) values in the range of -9.2 ~ -1.7 and Mg# of <50, and higher TiO2 contents (>2 wt.%), indicating that they were generated directly from a peripheral zone of the mantle plume by low degree of melting. The mafic-ultramafic intrusions have higher εNd(t) of -1.3 ~ 11.2 and Mg# of 33 ~ 90, and lower TiO2 < 1.8 weight percent, suggesting that their parental magmas were produced from lithospheric mantle source by high degree of melting due to higher temperature of the mantle plume head. A possible mantle plume model beneath lithospheric mantle of the Tarim Basin, Tianshan and Beishan and its spatial framework is suggested. [ABSTRACT FROM AUTHOR]

• TWJ-E2 vaccine can confer full protection against lethal challenge of CSFV genotype 2 field isolates. • TWJ-E2 vaccination elicited high levels of E2 and neutralizing antibodies. • TWJ-E2 vaccination eliminated the virus and prevented viral shedding after CSFV challenge. Classical swine fever (CSF) still threatens the swine industry in China, with genotype 2 isolates of CSFV dominating the epizootics. In 2018 the first E2 subunit marker vaccine against CSFV (Tian Wen Jing, TWJ-E2®), containing a baculovirus-expressed E2 glycoprotein of a genotype 1.1 vaccine strain, was officially licensed in China and commercialized. To evaluate the cross-protective efficacy of TWJ-E2 against different virulent genotype 2 Chinese field isolates (2.1b, 2.1c, 2.1 h, and 2.2), 4-week-old pigs were immunized with the TWJ-E2 vaccine according to the manufacturer's instructions and then challenged with genotype 2 strains. A group vaccinated with the conventional C-strain vaccine was included for comparison. TWJ-E2 vaccinated pigs developed higher levels of E2 and neutralizing antibodies than those receiving the commercial C-strain vaccine. All TWJ-E2 and C-strain vaccinated pigs survived challenge without development of fever, clinical signs or pathological lesions. In contrast, all unvaccinated control pigs displayed severe CSF disease with 40–100% mortalities by 24 days post challenge. None of the TWJ-E2 and C-strain vaccinated pigs developed viremia, viral shedding from tonsils, Erns protein in the sera, or viral RNA loads in different tissues after challenge, all of which were detected in the challenged unvaccinated controls. We conclude that vaccination of young pigs with TWJ-E2 provides complete immune protection against genotypically heterologous CSFVs and prevents viral shedding after challenge, with an efficacy at least comparable to that elicited by the conventional C-strain vaccine. [ABSTRACT FROM AUTHOR]

Pregnant women infected with HCV should be given attention due to their special physiological stage and the effect on offspring health. To examine the prevalence of HCV infection among pregnant women in part of China and explore relevant factors during pregnancy, a cross-sectional study was conducted in four maternal and children health care institutions (MCHC) in Guangdong, Hunan and Chongqing. Pregnant women who were delivered, induced or spontaneous abortion were included and relevant information was collected through the Hospital Information System. Results showed that the prevalence of HCV among pregnant women in four MCHCs was 0.11% (95% CI 0.09-0.13%). Age, occupations, regions, syphilis-infection, intrahepatic cholestasis of pregnancy (ICP), and placenta previa were significant factors (all P < 0.05). Age and syphilis-infection were positively correlated with HCV infection (Z = 3.41, P = 0.0006; OR = 18.16, 95% CI 9.34-35.29). HCV and HBV infection were risk factors of ICP (OR = 4.18, 95% CI 2.18-8.04; OR = 2.59, 95% CI 2.31-2.89). Our study indicates that the prevalence of HCV among pregnant women in the three provinces(city) was low compared with the general population in China. Older age and syphilis-infection increased the risk of HCV infection during pregnancy. HCV infection was a risk factor of ICP. Generally, we need keep a watchful eye on HCV infection and relevant factors mentioned above during pregnancy in clinic, especially those also infected with syphilis. HCV testing based on risk factors is recommended in antenatal care and obstetrics., (© 2023. The Author(s).)