1. Identification and expression profiles of IL-8 in bighead carp (Aristichthys nobilis) in response to microcystin-LR.
- Author
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Li H, Cai Y, Xie P, Li G, Hao L, and Xiong Q
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Carps metabolism, China, DNA, Complementary genetics, DNA, Complementary metabolism, Fish Proteins chemistry, Fish Proteins metabolism, Interleukin-8 chemistry, Interleukin-8 metabolism, Lakes, Marine Toxins, Molecular Sequence Data, Nucleic Acid Amplification Techniques, Phylogeny, RNA, Messenger genetics, RNA, Messenger metabolism, Real-Time Polymerase Chain Reaction, Sequence Alignment, Tissue Distribution, Carps genetics, Environmental Exposure, Fish Proteins genetics, Interleukin-8 genetics, Microcystins toxicity, Transcriptome drug effects
- Abstract
Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp.
- Published
- 2013
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