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Seasonal polyphenism is a phenomenon where two or more phenotypes are produced from a single genotype in different seasons. This phenomenon, a special type of phenotypic plasticity, is one of the major reasons for the success of insects and is also one of the primary sources of synonymy generations in insect taxonomy. Two nominal scorpionflies, Panorpa liui Hua, 1997 and Panorpa jilinensis Zhou, 2000 from the northeastern region of China, are very similar in morphology except for body coloration. However, whether they are conspecific or distinct species remains to be resolved. Here, we investigated these two nominal species through integrative taxonomy, including morphological variation analyses, geometric morphometrics of wing size and shape variations, genetic diversity, phylogenetic analyses based on two mitochondrial (COI and COII) and one nuclear (28S rRNA) gene fragments, and biological rearing. The results show that P. liui Hua, 1997 is a bivoltine species of seasonal polyphenism in the lowland plain, exhibiting a black morph in the spring and a yellow morph in the summer. Panorpa jilinensis Zhou, 2000 syn. nov. is considered as a junior synonym of P. liui Hua, 1997. The value of integrative taxonomy for species delimitation is discussed. [ABSTRACT FROM AUTHOR]

Strew pellet production not only contribute to regional sustainable development and localized energy transition, but also help to mitigate global greenhouse gas emissions. With the development strew pellet products in China, it is critical to uncover the embodied emissions, land use and economic cost effectiveness from producing strew pellet. In order to reach such a target, two main categories of biomass pellet production including a large-scale centralized factory and a small-scale distributed workshop are investigated. Compared with raw coal production, the unit co-benefits in terms of per gigajoule of straw pellets from centralized factory are 1687 kg CO 2 , 8.65 g SO 2 , 3.21 g NOx, and 3.897 g PM 10 , and 0.33 m2 land use, and those for straw pellets from centralized factory are 1352 kg CO 2 , 8.46 g SO 2 , 3.12 g NOx, and 4.22 g PM 10 , and 0.33 m2 land use. Cost-effectiveness for the two straw pellets production system were also uncovered so that the relevant interested agents such as decision makers, business investors or environmental researchers can see the potential economic performance from developing such kind biomass plants. We conclude that environmental performance of the straw pellets whether from centralized factor or decentralized workshop have attractive alternatives to coal production. Image 108297 • LCA-based co-benefits approach was applied to investigate biomass pellet production. • The analysis framework includes energy consumption, equipment and infrastructure. • Emission factors and land demand for producing one-unit products are presented. • Environmental performance of straw pellets has attractive alternatives to raw coal. • Electricity is an important factor affecting the performance of pellets production. [ABSTRACT FROM AUTHOR]

To evaluate the effectiveness and safety of hypothermic perfusion in the phacoemulsification of hard nuclear cataract. Tertiary opthalmology center, China. Laboratory study and prospective randomized clinical trial. Rabbits and patients with hard nuclear cataract underwent phacoemulsification with perfusion temperatures at 4°C or 24°C. Anterior segment optical coherence tomography (AS-OCT), corneal endothelial cell count (ECC), and cornea sections were observed before the rabbits' operation and 1 day and 7 days postoperatively. AS-OCT, corneal ECC, and anterior chamber (AC) inflammation were observed before the patients' operation and 1 day, 7 days, and 30 days postoperatively. The study comprised 40 rabbits and 80 patients. In the animal models, the mean central corneal thickness (CCT) in the 4°C group (370.4 μm ± 45.5 [SD]) was thinner than in the 24°C group (496.7 ± 121.5 μm) 1 day postoperatively (P <.001). The mean AC inflammation reaction grade in the 4°C group (1.1 ± 0.9) was lower than in the 24°C group (2.2 ± 0.8) (P =.0333). In clinical trials, the mean CCT and incisional corneal thicknesses in the 4°C group (600.7 ± 51.8 μm and 859.2 ± 177.8 μm, respectively) were thinner than in the 24°C group (655.3 ± 85.0 μm and 955.9 ± 196.7 μm, respectively) (P <.001). The endothelial cell density (P =.036) and hexagonality (P =.001) were higher in the 4°C group. The mean AC inflammation reaction grade in the 4°C group (0.6 ± 0.6) was lower than in the 24°C group (1.3 ± 1.0) 1 day postoperatively (P =.004). Hypothermic perfusion in phacoemulsification of hard nuclear cataract is safe and it can effectively protect corneal endothelium, decrease corneal edema, and reduce AC inflammation in the early postoperative stage. [ABSTRACT FROM AUTHOR]

China's ties with Africa are evolving into a multi-faceted relationship of increasing complexity. After nearly two decades of debt-financed infrastructure development, Beijing's exposure to African debt is reaching disquieting proportions with an estimated US$132 billion owed to China in 2016. Managing this new role as Africa's creditor poses uncomfortable questions for creditor and debtor alike. Concurrently, the quiet surge of Chinese investment in manufacturing in Africa is transforming local economies in ways that are beginning to alter the continent's position within the global economy. Finally, the proliferation of Chinese businesses and migrants across Africa is inspiring greater Chinese involvement in UN peacekeeping and private security initiatives. This article examines how these structural changes are challenging core practices and principles which guided China–Africa relations in its formative decades. For instance, under the banner of an alternative to western policies China promoted the absence of conditionalities attached to its concessional loans and grants. Equally, promotion of industrialization of African economies marks a key shift away from China's resource-centric engagement with the continent. And, in the case of security, Beijing's commitment to avoid intervention in domestic affairs is being set aside with implications for its principles, and ultimately status, in Africa. [ABSTRACT FROM AUTHOR]

Aim: To investigate the features of skip lesions and evaluate value of top‐hat procedure in management of squamous intraepithelial lesion. Methods: We reviewed the records of patients who underwent loop electrosurgical excision procedure (LEEP) in Peking University First Hospital between 2011 and 2016. Patients were confirmed to have CIN1–3. The term 'skip lesion' refers to lesion lying deep in cervical canal discontiguous with other lesions in transformation zone and was confirmed by top‐hat. We compared their lesion grade in patients with or without skip lesion using logistic regression. We further reviewed patients who underwent subsequent hysterectomy within 6 months following LEEP and evaluated if top‐hat procedure led to less residual lesions or was able to predict residual lesions. Results: A total of 2260 patients were included and 595 underwent top‐hat procedure. Thirty‐nine out of 595 patients had skip lesions (6.5%), among whom two patients had CIN1 (5.1%), eight had CIN2 (20.5%) and 29 had CIN3 (74.4%). Logistical regression showed CIN3 was associated with higher risk of skip lesions compared to CIN1 (OR = 4.433, 95%CI: 1.036–18.964), while CIN2 was not (OR = 1.762, 95%CI: 0.366–8.471). Sixty‐two patients underwent hysterectomy within 6 months following LEEP (CIN1–3), 24 underwent top‐hat. Analysis revealed top‐hat procedure did not result in less residual lesions. Colposcopy impression or prior HPV test was unable to predict skip lesions. Conclusion: About 9.4% patients with CIN3 had skip lesions in the study, which is associated with elevated risk for residual lesion. Top‐hat procedure is able to detect skip lesions, but should not be performed on routinely because its prognostic value is not proved. [ABSTRACT FROM AUTHOR]

Conclusions: There could be another candidate gene in DFNA2, which could be responsible for the hearing loss phenotype. Objective: We collected a four-generation family from the southern part of China with autosomal dominant sensorineural hearing impairment. In order to identify the responsible pathogenic mutations in this family, we set out to identify the locus and to sequentially analyze the candidate genes in the identified region. Methods: After family ascertainment and clinical analysis, exclusive analysis was performed. Then a genome-wide scan was performed using an Illumina Linkage-12 DNA Analysis Kit (average spacing 0.58 cM). Fine-mapping markers were genotyped to identify the locus. Finally, we performed haplotype analyses and candidate gene DNA sequencing for the family. Results: The known genetic loci and genes were not associated with our family. The genome-wide scan and haplotype analyses traced the disease to chromosome 1p34.2-p34.3 with maximum multi-point LOD score of 3.2, which overlaps with DFNA2. We failed to identify any of the known or novel variants within KCNQ4, a voltage-gated potassium channel gene, and GJB3, a gene that encodes the gap junction protein connexin 31, which were the cloned deafness genes in DFNA2. [ABSTRACT FROM AUTHOR]

Conclusions: Analysis of the complete mtDNA genome and X-linkage of this five-generation Chinese family revealed that the 1555A > G mutation may lead to deafness. Objectives: Mutations in mitochondrial DNA (mtDNA) have been found to be associated with sensorineural hearing loss. However, the variable clinical phenotype and incomplete penetrance of mtDNA 1555A > G-induced hearing loss complicate our understanding of this mutation. We aimed to identify whether nuclear genes, mitochondrial haplotypes/variants, and a possible threshold effect are involved in its manifestation in the pedigree. Methods: We performed clinical, genetic, and X-linkage analysis of a five-generation Chinese family in which all the affected individuals were male. Results: Clinical evaluation revealed that affected individuals with or without aminoglycoside exposure developed hearing loss extending gradually from 8000 Hz to 4000 Hz and then to 1000 Hz. Using X-linkage analysis and sequencing, we detected an identical homoplasmic 1555A > G mutation in nine individuals, and a previously unreported variant 14163C > T in mtDNA. The new variant 14163C > T coexisted with the 1555A > G mutation in six affected subjects of our pedigree. The previously unreported variant 14163C > T and aminoglycoside exposure may synergize the development of this deafness. [ABSTRACT FROM AUTHOR]

Abstract: Objective: We analyzed the clinical features and family-related gene mutations for the first two Chinese cases of type IV Waardenburg syndrome (WS4). Methods: Two families were analyzed in this study. The analysis included a medical history, clinical analysis, a hearing test and a physical examination. In addition, the EDNRB, EDN3 and SOX10 genes were sequenced in order to identify the pathogenic mutation responsible for the WS4 observed in these patients. Results: The two WS4 cases presented with high phenotypic variability. Two novel heterozygous mutations (c.254G>A and c.698-2A>T) in the SOX10 gene were detected. The mutations identified in the patients were not found in unaffected family members or in 200 unrelated control subjects. Conclusions: This is the first report of WS4 in Chinese patients. In addition, two novel mutations in SOX10 gene have been identified. [Copyright &y& Elsevier]

Abstract: Waardenburg syndrome (WS) is a rare disorder characterized by distinctive facial features, pigment disturbances, and sensorineural deafness. There are four WS subtypes. WS1 is mostly caused by PAX3 mutations, while MITF, SNAI2, and SOX10 mutations are associated with WS2. More than 100 different disease-causing mutations have been reported in many ethnic groups, but the data from Chinese patients with WS remains poor. Herein we report 18 patients from 15 Chinese WS families, in which five cases were diagnosed as WS1 and the remaining as WS2. Clinical evaluation revealed intense phenotypic variability in Chinese WS patients. Heterochromia iridis and sensorineural hearing loss were the most frequent features (100% and 88.9%, respectively) of the two subtypes. Many brown freckles on normal skin could be a special subtype of cutaneous pigment disturbances in Chinese WS patients. PAX3, MITF, SNAI2, and SOX10 genes mutations were screened for in all the patients. A total of nine mutations in 11 families were identified and seven of them were novel. The SOX10 mutations in WS2 were first discovered in the Chinese population, with an estimated frequency similar to that of MITF mutations, implying SOX10 is an important pathogenic gene in Chinese WS2 cases and should be considered for first-step analysis in WS2, as well as MITF. [Copyright &y& Elsevier]

Abstract: Jujube (Ziziphus jujuba Mill.) has long been used for human consumption and medicinal purposes in China. It is recommended for some diseases in which radical species are produced as a result of oxidative stress. However, the systematic study on the antioxidant capacities of various tissues of jujube is still lacking. In order to address this, various tissues of three jujube varieties were characterized with respect to their antioxidant capacities and antioxidant compounds such as flavonoid, ascorbic acid, anthocyanin and phenolic. Antioxidant capacity was measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric-reducing antioxidant power) assays. The phenolic acids were analyzed by high-performance liquid chromatography (HPLC). The results show that the peel of all cultivars has the highest antioxidant capacities, reflecting the highest content of total phenolics, flavonoids, and anthocyanins found in this part. In addition, the predominant phenolic acid in jujube was found to be protocatechuic acid, followed by gallic acid, chlorogenic acid and caffeic acid. The results obtained in this study clearly indicate that Chinese jujube has a significant potential to use as a natural antioxidant agent. [Copyright &y& Elsevier]

Based on household-level survey data collected from Qinghai Province of China, we estimated the greenhouse gas (GHG) and air pollutant emissions from spatial perspectives, including household energy consumption in pastoral, agropastoral, and agricultural zones. The findings suggest that the total annual GHG and pollutant emissions per capita in the area was 2296.32 kg per year. The highest amount of pollutants was emitted from the pastoral zones, followed by the agropastoral and agricultural zones. CO 2 is the primary GHG emitted by household energy consumption. Dung burning was the cause of the high PM 2.5 emissions in the pastoral areas, while the use of coal was the primary cause of GHG and pollutant emissions in the agropastoral and agricultural zones. • We used empirical data from rural households in Qinghai, China. • This paper indicates the reliance of local energy consumption on non-commercial energy. • We estimate greenhouse gas and pollutant emissions resulting from solid-fuel combustion. • We compare indoor pollutant emissions from pastoral, agropastoral, and agricultural zones spatially. • This paper provides policy recommendations for energy transition towards clean energy. Energy consumption in the household sector has become an important issue in China's energy consumption and an important unit of China's clean energy transformation. Currently, the potential air pollution, carbon emissions and health risks caused by energy consumption in many areas cannot be ignored, and refined and regionalized index-based research data necessary to support decision making are lacking. Based on household-level survey data collected from Qinghai Province, China, we estimated greenhouse gas (GHG) and air pollutant emissions from spatial perspectives, including household energy consumption in pastoral, agropastoral, and agricultural zones. The findings suggest that the total annual GHG and pollutant emissions per capita in the area was 2296.32 kg per year. The highest amount of pollutants was emitted from the pastoral zones, followed by the agropastoral and agricultural zones. CO 2 is the primary GHG emitted by household energy consumption. Dung burning was the cause of the high PM 2.5 emissions in the pastoral areas, while the use of coal was the primary cause of GHG and pollutant emissions in the agropastoral and agricultural zones. These findings highlight the need to integrate household energy policies with rural development to enable a complete transition towards cleaner fuels. [ABSTRACT FROM AUTHOR]

The term "left-behind adolescent" refers to adolescents who remain in the household registration area to live with their one parent or temporary guardian because both or one of their parents have left town for work. Subjective vitality is a crucial indicator of healthy adolescent growth. However, few studies have been conducted on the factors associated with the left-behind adolescents' subjective vitality. This study investigated the level of subjective vitality of left-behind adolescents and explored the potential mechanisms between the parent-child relationship and subjective vitality. We collected a sample of 604 secondary school students from a rural region in southwest China (52.98% female; Mage = 13.76; SD = 0.88). We compared a mediation model among adolescents left behind by fathers (N = 200), mothers (N = 122), and both parents (N = 282). The results found that parent-child relationships were positively associated with subjective vitality. Basic psychological needs and meaning in life both mediated the relationship between the parent-child relationship and subjective vitality among left-behind adolescents. There was no significant difference in the role of the father-child relationship and the mother-child relationship. In addition, unlike previous research, we discovered no direct association between the mother-child relationship and subjective vitality when mothers were absent. And there was no significant direct relationship between the father-child relationship and subjective vitality when fathers were absent. Our findings add incremental insight into how parental absence affects left-behind adolescents' positive psychological development. Highlights: The left-behind adolescents' subjective vitality was strongly and positively related to parent-child relationships (the father-child and the mother-child relationship), BPNs, and meaning in life. BPNs and meaning in life both mediated the relationship between the parent-child relationship and subjective vitality among left-behind adolescents. There was no significant difference in the role of the father-child relationship and the mother-child relationship. This study illustrates that the absent parent cannot provide the left-behind adolescents with the necessary psychological support to direct promote subjective vitality. In contrast, only the remaining parent would promote the adolescents' sense of vitality. [ABSTRACT FROM AUTHOR]

Tipuloidea (crane flies) is one of the most species-rich groups of Diptera, including over 15,000 species worldwide, with a wide variety of habitats. However, relatively less attention has been paid to the larvae of crane flies in contrast to the better-known adults. In this study, Tipula (Pterelachisus) cinereocincta mesacantha Alexander, 1934 is added to the fauna of China, from larvae found beneath a piece of moss in a forest of eastern Liaoning and reared and associated with the adult stage. The external structures of the larva are described thoroughly accompanied by light microscopy photographs. In addition, with scanning electron microscopy, more detailed structures of the larval head capsule, the antenna and the mouthparts are presented for the first time for the larvae of Tipula (Pterelachisus). Some microscopic structures, including the paired peg-like sensilla on the central teeth of epipharynx, and a pair of cushiony lobes anterior to the teeth, are newly recorded for the larva of Tipuloidea. On the basis of the comparison of larval characters, the described larvae of Pterelachisus are roughly categorized into three groups. The variations of larval heads between T. (P.) cinereocincta mesacantha and other related species are also discussed. This discovery will provide new data and insights for further research on the taxonomy and phylogeny of Tipuloidea, as well as some evidence on the biology and adaptions of crane fly larvae. [ABSTRACT FROM AUTHOR]

• We develop a new model of economic growth that as a starting point the traditional neoclassical growth model RCK(Ramsey-Cass-Koopmans) • We examine the effect of government tax and fiscal expenditure structure on economic growth. • We find that income tax rate, allocation of government fiscal expenditure is inverted "U" in relation to the economic growth rate. • The finding helps the government to form more rational technological innovation preferences in decision-making. China's economy has been transitioning from labor-driven and capital-driven at a stage of rapid growth to innovation-driven at a stage of high-quality development. In this paper, government's "technological innovation preference" (TIP) is introduced into the traditional neoclassical economic growth model which is Ramsey-Cass-Koopmans (RCK), as a factor affecting the utility of endogenous growth model with capital accumulation and technological innovation. Assumed that the government fiscal revenue is used for public and research spending, through which we can examine its impact on high-quality economic growth and transmission mechanism, as well as how TIP influences on the economy. Results indicate that the tax rates and the growth rates would be raised, while the proportion of public spending would fall if TIP enhanced by the government. Furthermore, though steady-state output level may be lower in short term, however, it may also achieve high-quality growth in long run. [ABSTRACT FROM AUTHOR]

Background: Transversus abdominis plane (TAP) block can provide effective analgesia for abdominal surgery. However, it was questionable whether TAP had additional effect in the context of multimodal analgesia (MMA). Therefore, this study aimed to assess the additional analgesic effect of preoperative TAP block when added to MMA protocol in open gynecological surgery. Methods: In this prospective, randomized-controlled trial, 64 patients scheduled for open gynecological surgery were randomized to receive preoperative TAP block (Study group, n = 32) or placebo (Control group, n = 32) in addition to MMA protocol comprising dexamethasone, acetaminophen, flurbiprofen and celecoxib, and rescued morphine analgesia. The primary outcome was rescued morphine within 24 h after surgery. Secondary outcomes included pain scores, adverse effects, quality of recovery measured by 40-item quality of recovery questionnaire score (QoR-40) at 24 h, and quality of life measured with short-form health survey (SF − 36) on postoperative day (POD) 30. Results: The Study group had less rescued morphine than the control group within 24 h [5 (2–9) vs. 8.5 (5–12.8) mg, P = 0.013]. The Study group had lower pain scores at 1 h [3 (2–4) vs. 4 (3–5), P = 0.007], 2 h [3 (2–4) vs. 3.5 (3–5), P = 0.010] and 6 h [3 (2–3) vs. 3 (2.3–4), P = 0.028], lower incidence of nausea at 48 h (25.8% vs. 50%, P = 0.039), and higher satisfaction score [10 (10–10) vs. 10 (8–10), P = 0.041]. The SF-36 bodily pain score on POD 30 was higher in the Study group (59 ± 13 vs. 49 ± 16, P = 0.023). Conclusions: Preoperative TAP block had additional analgesic effect for open gynecological surgery when used as part of multimodal analgesia. Rescued morphine within 24 h was significantly reduced and the SF-36 bodily pain dimension at 30 days after surgery was significantly improved. Trial registration: www.chictr.org.cn (ChiCTR2000040343, on Nov 28 2020). [ABSTRACT FROM AUTHOR]

Atractylodes lancea (Thunb.) DC. is an important medicinal plant mainly distributed in China. A. lancea is rich in volatile oils and has a significant effect on various diseases, including coronavirus disease 2019 (COVID-19). Based on the signature constituents of volatile oils, A. lancea is divided into two chemotypes: the Dabieshan and Maoshan chemotype. Gas chromatography-mass spectrometry (GC–MS) results revealed that the hinesol and β-eudesmol contents in the Dabieshan chemotype were higher than those in the Maoshan chemotype. Next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing technologies were combined to investigate the molecular mechanisms of sesquiterpenoid biosynthesis in A. lancea. A total of 42 differentially expressed genes (DEGs) for terpenoid biosynthesis were identified in the two chemotype groups, and nine full-length terpene synthase (TPS) genes were identified. Subcellular localization revealed that AlTPS1 and AlTPS2 proteins were localized in the nucleus and endoplasmic reticulum. They use FPP as a substrate to generate sesquiterpenoids. AlTPS1 catalyzes biosynthesis of elemol while AlTPS2 is observed to perform β-farnesene synthase activity. This study provides information for understanding the differences in the accumulation of terpenoids in two chemotypes of A. lancea and lays a foundation for further elucidation of the molecular mechanism of sesquiterpenoid biosynthesis. [ABSTRACT FROM AUTHOR]

Considering the significance and importance of urban sustainability in China and China's history of rapid urbanisation and industrialisation, this paper aims to incorporate the investigation of climate change and air pollution in urban sustainability research in China through a driver-pressure-state-impact-response model. The model has 12 indices: population, enterprise, registered vehicles, energy consumption, emissions and concentrations of carbon dioxide and sulfur dioxide, land use change, annual temperature, tropical nights and reforestation were selected. It was used in the analysis of 14 cities in the Liaoning Province. The results show that the model is an effective tool for urban sustainability research, allowing for the investigation of interactions of climate change and air pollution and contributing to the body of knowledge by aiding generation of realistic and practical measures. [ABSTRACT FROM AUTHOR]

• Grubs, larvae of family Scarabaeidae, are used in East-Asian herbal medicine. • DNA metabarcoding was applied to 11,539 Grubs. • Identified 21 amplicon sequence variants from Grubs species. • Identified 28 amplicon sequence variants from non-Grubs species. • These finding urges stringent quality measure to ensure human safety and drug efficacy. Grubs, called Qicao in China, have a long tradition as herbal medicine in East Asia. These larvae belong to the diverse family Scarabaeidae and are typically harvested from the wild during their immature stage based on morphological characteristics. However, rapid and accurate identification becomes challenging when relying solely on external morphological features, as the lack of clarity on biological sources raises safety concerns for clinical applications. The application of DNA metabarcoding provides a solution by enabling the determination of the biological source of a large sample. In the current study, we collected 19 batches of Grubs, consisting of 11,539 individuals, from the market and analyzed their biological composition through metabarcoding. We identified 49 Amplicon Sequence Variants (ASVs), 21 of which were Grubs. The 21 ASVs were classified into seven Molecular Operational Taxonomic Units (MOTUs) through species delimitation, which revealed that commercially available Grubs are predominantly sourced from Protaetia brevitarsis seulensis , while species of Rutelinae, Anomala , and Holotrichia were also abundant in some commercial batches. Among the identified ASVs, 28 belonged to non-Grub species and indicated adulteration from different animal families; high abundances of these ASVs were detected for Bombycidae, Tabanidae, and Viviparidae. Our findings underscore the complexity of Grubs' species composition and advocate for a deeper understanding of the wildlife sources contributing to herbal products. This research contributes valuable insights into the molecular identification of Grubs, paving the way for enhanced quality assurance in traditional medicine applications to provide safe and effective medicines for humanity. [ABSTRACT FROM AUTHOR]

Objective: The aim of this study was to evaluate the GoldenGate microarray as a diagnostic tool and to elucidate the contribution of the genes on this array to the development of both nonsyndromic and syndromic sensorineural hearing loss in China. Methods: We developed a microarray to detect 240 mutations underlying syndromic and nonsyndromic sensorineural hearing loss. The microarray was then used for analysis of 382 patients with nonsyndromic sensorineural hearing loss (including 15 patients with enlarged vestibular aqueduct syndrome), 21 patients with Waardenburg syndrome, and 60 unrelated controls. Subsequently, we analyzed the sensitivity, specificity, and reproducibility of this new approach after Sanger sequencing-based verification, and also determined the contribution of the genes on this array to the development of distinct hearing disorders. Results: The sensitivity and specificity of the microarray chip were 98.73% and 98.34%, respectively. Genetic defects were identified in 61.26% of the patients with nonsyndromic sensorineural hearing loss, and 9 causative genes were identified. The molecular etiology was confirmed in 19.05% and 46.67% of the patients with Waardenburg syndrome and enlarged vestibular aqueduct syndrome, respectively. Conclusion: Our new mutation-based microarray comprises an accurate and comprehensive genetic tool for the detection of sensorineural hearing loss. This microarray-based detection method could serve as a first-pass screening (before next-generation-sequencing screening) for deafness-causing mutations in China. [ABSTRACT FROM AUTHOR]

Farmers and their energy saving behaviors make significant sense for long-run sustainability and enacting effective energy policies, particularly in the fragile eco-environment and non-developed rural area. This study investigates the rural household energy-saving behaviors and influencing factors in the loess hilly region of China by taking Qin'an county in Gansu province based on a total of 506 households by adopting the participatory farmer assessment method. Most peasant families pay close attention energy conservation in their daily lives. Most farmer households (88.74%) were conscious of energy saving in their daily lives, and consider energy saving as a self-acting behavior (68.58%) and good habit (87.94%); electricity is the main clean energy for farmers; the price of energy-saving products is the key factor for farmers, and the female and the younger householders have better performance in energy conservation. Farmers' energy-saving behaviors are negatively correlated with the altitude of their dwellings but positively correlated with the education level of householders, family size, income. We argue that continuous training on energy saving is necessary and should be tailored to local conditions and supported by the government. • A rural household survey of 506 households was conducted in the loess hilly region of China. • Female, younger, and higher education level shows positive impacts on energy saving. • Energy-saving behaviors positively correlated with income, health conditions, environmental concerns, and energy availability and prices. • The training efforts for promoting energy saving of rural households should be enhanced. • We argue that government subsidies should integrate into the promotion of energy-saving products. [ABSTRACT FROM AUTHOR]

• PM, SO 2 , CO reduced significantly, NO 2 was less clear, and O 3 increased significantly. • Excess health risks (ER) based on WHO guideline are two times than those from CAAQS guideline. • PM 10 rather than PM 2.5 contributed the most to ER. • ~15%, ~85% and ~95% people in YRD, FWP and JJJ were exposed to polluted air (HAQI > 100) in 2018. • Population-normalized HAQI significantly added the inequality of people exposure to polluted air. Air pollution events occurred frequently in China, and tremendous efforts were devoted to the reduction of air pollution in recent years. Here, analysis of ambient monitoring data of six criteria air pollutants from 367 Chinese cities during 2015–2018, showed that PM 2.5 , PM 10 , SO 2 and CO were reduced significantly by 22.1%, 13.5%, 46.4% and 21.5%, respectively, NO 2 reduction was less significant (6.3%) while O 3 level instead increased over China (13.7%). Spatial distribution, seasonal, monthly and diurnal variations of the air pollutants during 2018, implicated of effective control measures, were discussed in details, especially for the five key densely populated regions of Jing-Jin-Ji (JJJ), Fen Wei Plains (FWP), Yangtze River Delta (YRD), Sichuan Basin (SCB) and Pearl River Delta (PRD). Moreover, excess health risks (ERs) of the six pollutants were estimated for 2018, and such risks was two times higher if the World Health Organization (WHO) air quality guideline rather than Chinese guideline was adopted. PM 10 rather than PM 2.5 was the dominant contributor to ERs, and the case with both PM 2.5 and PM 10 exceeding threshold values occupied ~1/3 of total days, yet contributed ~2/3 of total ERs. For 2018, the health-risk based air quality index (HAQI) was further calculated by combining health risks from multiple pollutants, and it was found that high HAQI mostly distributed in North China Plain (NCP). ~15%, ~85% and ~95% people in YRD, FWP and JJJ were exposed to polluted air (HAQI > 100), and population-normalized HAQI further added the inequality, JJJ and a small region of SCB had much higher HAQI (>280). Investigations on HAQI with socioeconomic factors show that total population, population density and built-up area presented an inverted U-shape, suggesting existence of Environmental Kuznets Curve (EKC), while a positive relationship was found between HAQI and share of secondary industry. Multiple regression analysis suggested that built-up area was the most prominent factor to HAQI, followed by the gross domestic product (GDP). The findings here demonstrate in great details the current characteristics of air pollution and its associated health risks in China, therefore providing important implications for effective air pollution control strategies in near future for different regions of China. [ABSTRACT FROM AUTHOR]

Hearing loss (HL) is a common sensory disorder. More than half of HL cases can be attributed to genetic causes. There is no effective therapy for genetic HL at present, early diagnosis to reduce the incidence of genetic HL is important for clinical intervention in genetic HL. Previous studies have identified 111 nonsyndromic hearing loss genes. The most frequently mutated genes identified in NSHL patients in China include GJB2, SLC26A4, and the mitochondrial gene MT-RNR1. It is important to develop HL gene panels in Chinese population, which allow for etiologic diagnosis of both SHL and NSHL. In this study, a total of 220 unrelated Han Chinese patients with bilateral progressive SNHL and 50 unrelated healthy controls were performed Single nucleotide polymorphism (SNP) genotyping using an improved multiplex ligation detection reaction (iMLDR) technique, is to simultaneously detect a total of 32 mutations in ten HL genes, covering all currently characterized mutations involved in the etiology of nonsyndromic or syndromic hearing loss in the Chinese population. The 49 positive samples with known mutations were successfully detected using the iMLDR Technique. For 171 SNHL patients, gene variants were found in 57 cases (33.33%), among which, 30 patients carried mutations in GJB2, 14 patients carried mutations in SLC26A4, seven patients carried mutations in GJB3, and six patients carried mutations in MT-RNR1. The molecular etiology of deafness was confirmed in 12.9% (22/171) of patients carried homozygous variants. These results were verified by Sanger sequencing, indicating that the sensitivity and specificity of the iMLDR technique was 100%. We believe that the implementation of this population-specific technology at an efficient clinical level would have great value in HL diagnosis and treatment. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship of muscle mass and muscle function with age.Methods and Study Design: The study including 415 participants (aged 60-99 years). Upper (UMM) and lower (LMM) limbs muscle mass and whole body fat free mass (FFM) were measured by bioelectrical impedance analysis. The appendicular skeletal muscle mass (ASM) index (ASM/height2) was calculated. Muscle function was assessed by measuring hand grip strength (HGS) and gait speed.Results: Using ASM index cutoff values we found that higher prevalence of sarcopenia in women than in men (33.5% vs 23.6%, p=0.025). In the upper limb, HGS (β=-0.809) declined more rapidly with age than did UMM (β=-0.592) in men, but not in women (β=-0.389 and β=-0.486 respectively). In the lower limb, gait speed declined more rapidly than LMM in both men (β=-0.683 vs β=-0.442) and women (β=-1.00 vs β=-0.461). The variance of UMM explained 28-29% of the variance of HGS, and LMM explained 7-8% of the variance of gait speed in women and men respectively. In addition to the common predictors (BMI and age), the specific predictors were smoking, exercise and education for FFM and ASM, and smoking, drinking and exercise for HGS (p<0.05).Conclusions: Loss of muscle function is greater than the decline of muscle mass particularly in the upper limbs in men. However, women are more prone to have low muscle mass than the men. Exercise programs need to be designed gender specifically. [ABSTRACT FROM AUTHOR]

The characteristics of wine phenolics found in several North American and (for comparison) European grape cultivars grown in China were analyzed. This was done to find non-Vitis vinifera wines with prominent features in order to diversify the kinds of wines. The phenolic richness and antioxidant activity decreased in the order: red > rose > white wines. In the red wines, the American grape 'Cynthiana' had the highest total concentrations of phenols, anthocyanins, flavonols and phenolic acids, as well as antioxidant capacity, followed by the French hybrid 'Chambourcin', the lowest were detected in two European grape varieties, 'Merlot' and 'Cabernet Sauvignon', while the total flavon-3-ols levels were reversed among these red grape cultivars. The highest concentration of stilbenes out of all the wines analyzed was found in the 'Merlot' variety. There were significant differences among wine phenolic compositions between North American and European grape cultivars. The antioxidant activities were significantly related to the concentrations of total phenols (r² = 0.996), anthocyanins (r² = 0.984), flavonols (r² = 0.850) and gallic acid (r² = 0.797). The prominent features of wine aroma and nutrition could make the American grape wines attractive to consumers. It is therefore necessary to perform further research on cultural practices and wine making involving these grapes. [ABSTRACT FROM AUTHOR]

Abstract: This study provides the first intensive investigation of Dichlorodiphenyltrichloroethanes (DDT) distribution in typical paint factories and shipyards in China where DDT containing antifouling paint were mass produced and used respectively. DDTs were analyzed in soil, sludge and sediment samples collected from three major paint factories and two shipyards. The results showed that the total DDTs concentrations detected in paint factory and shipyard sites ranged from 0.06 to 8387.24mgkg−1. In comparison with paint factory sites, the shipyard sites were much more seriously contaminated. However, for both kinds of sites, the DDTs level was found to be largely affected by history and capacity of production and use of DDT containing antifouling paint. (DDE+DDD)/DDT ratios indicated that DDT containing antifouling paint could serve as important fresh input sources for DDTs. It can be seen that most samples in shipyards were in ranges where heavy contamination and potential ecological risk were identified. [Copyright &y& Elsevier]

New Energy Vehicles (NEVs) exert great potential in reducing carbon emissions, improving air quality and reducing the dependence on petroleum products, and are also essential in realizing the social transformation toward a low-carbon society. Since public are the principal users and stakeholders of NEVs, it's vital to understand their acceptance of NEVs and discuss the influencing factors of public acceptance, so as to promote the development of NEVs. Based on the survey data of Gansu Province, this paper analyzes the public perception and acceptance of NEVs in underdeveloped regions, and explores main factors affecting their acceptance by adopting Structural Equation Model. The results show that the public acceptance of NEVs is relatively higher, with only 51.99% residents are willing to accept NEVs. And there are differences of public acceptance of NEVs among different groups. The acceptance of females, young and less educated public are relatively higher, decreases with aging and increases with education attainment. Perceived risk and perceived cost are significantly negative correlated with public acceptance of NEVs, while subjective norm and the quality of NEVs positively correlated with public acceptance. In addition, support policy is significantly positively correlated with perceived cost while perceived cost is positively corelated with perceived risk. Finally, this paper proposes countermeasures and suggestions on improving the public acceptance of NEVs in underdeveloped regions. • We conducted the NEVs acceptance of 1356 residents in Chinese underdeveloped regions. • 51.99% of the public in underdeveloped regions of China were willing to accept NEVs. • Perceived risk, perceived cost, and NEVs' quality are the key influencing factors. • Support policy, knowledge have significant indirect impact on acceptance of NEVs. • Enhancing subsidies and supporting facilities were effective ways. [ABSTRACT FROM AUTHOR]

Water shortage has become one of the most serious challenges faced by humans. Thus, improving water use behaviors and saving water are critical. In this paper, based on the extended Value-Belief-Norm (VBN) theory, we used the structural equation model and the survey data of 558 households in the Loess Hilly Region to analyze the key factors affecting water saving behaviors of farmers. The domestic water consumption of farmers in Loess Hilly Region is mainly for diet and personal hygiene. The water-saving behaviors of farmers are significantly affected by their willingness, knowledge and age. Personal habits, policy incentives, egocentric values and biospheric values indirectly affect the willingness to save water by acting on personal water-saving norms. Gender and annual household income also have a significant impact on water-saving willingness of farmers. The estimation results of multi-group structural equation model and full-sample structural equation model are generally consistent, and there are significant differences in the influence of gender and water source adjustment variables on water-saving behaviors among different hypothetical paths. Among them, female, cellar water and well water groups have the greater impact on water-saving behaviors. These results provide references for policy makers to formulate effective rural water-saving strategies. Policymakers should strengthen the propagandize of water-saving knowledge and skills, popularize knowledge about water resources and ecological environment changes, encourage farmers to develop good water use habits, and actively promote the subsidy system for water-saving appliances. Then they should establish a supervision and management mechanism of water resources to stimulate farmers to save water, which realize the sustainable use of local water resources. [Display omitted] • Investigate the water saving willingness and water saving behaviors of 558 farmers in the Loess Hilly Region. • There is a disconnection between farmers' water-saving willingness and their behaviors. • Gender and water source group models have significant differences in the influence paths of water-saving behaviors. • Personal habits, policy incentives and values play an important role in water-saving behaviors. • Enhancing government support will help to transform water saving willingness into actual water saving behaviors. [ABSTRACT FROM AUTHOR]

• We evaluate the implementation and potential impact of solar energy-based poverty reduction program in Qinghai, China. • PV stations, precisely planned to fulfill 'targeted' intervention, were aggregated into larger PV farms in implementation. • It is challenging to precisely target the poor, improve income, facilitate energy transition, and enhance local capacity. • Future energy-based development needs to sufficiently consider local contexts and promote local engagement. In 2014, China initiated a nationwide poverty reduction campaign through deploying photovoltaic (PV) systems in its vast rural areas, which aims to support over 2 million households. Despite the significant socioeconomic benefits anticipated by policy-makers, it remains unclear regarding the challenges of implementing such an ambitious program in remote rural areas and its effect on poverty reduction. Focusing on Qinghai, a province that serves as the testbed for solar energy development in China but suffers chronic poverty, we aim to assess implementation status of the program, and examine whether and how solar energy-based development affects rural household well-being and generates socioeconomic spillovers. Using data from multiple sources, including household survey, interviews, policy documents, and large-scale socioeconomic covariates, as well as advanced statistical and spatial analyses, we find there have been substantial adjustments to the plan of building small-scale PV stations across poor villages throughout Qinghai. While total installation capacity was met, the 97 planned PV stations in 39 counties were aggregated into 24 medium- to large-scale PV farms in 27 counties. We also find substantial challenges to precisely target the poor, improve their income, facilitate a transition in household use of energy, and enhance local capacity for economic development. Future PV-based intervention programs need to sufficiently consider local contexts and demands, and promote local engagement to achieve the twin goals of sustainable energy transition and poverty reduction. [ABSTRACT FROM AUTHOR]