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Length-weight relationships (LWRs) were reported for ten fish species (Abbottina rivularis, Hemibarbus labeo, Hemibarbus maculatus, Onychostoma macrolepis, Opsariichthys bidens, Rhynchocypris lagowskii, Schizothorax prenanti, Sinibrama macrops, Zacco platypus, and Siniperca scherzeri). Fishes were collected from 62 sampling stations covering an area of ∼7000 km2 in the Qinling Mountains, characterized by unique fauna and distribution pattern, using gillnets and ground cages during June to December 2022. The intercept a ranged from 0.013 for S. prenanti to 0.060 for H. labeo, and the slope b ranged from 2.537 for H. labeo to 3.079 for Z. platypus. [ABSTRACT FROM AUTHOR]

This paper introduces the South East Asia Mandarin-English corpus, a 63-h spontaneous Mandarin-English code-switching transcribed speech corpus suitable for LVCSR and language change detection/identification research. The corpus is recorded under unscripted interview and conversational settings from 157 Singaporean and Malaysian speakers who spoke a mixture of Mandarin and English within a single sentence. About 82 % of the transcribed utterances are intra-sentential code-switching speech and the corpus will be release by LDC in 2015. This paper presents an analysis of the code-switching statistics of the corpus, such as the duration of monolingual segments and the frequency of language turns in code-switch utterances. We also summarize the development effort, details such as the processing time for transcription, validation and language boundary labelling. Lastly, we present textual analyses of code-switch segments examining the word length of monolingual segments in code-switch utterances and the most common single word and two-word phrase of such segments. [ABSTRACT FROM AUTHOR]

A simulation-based interval quadratic programming (IQP) method was developed for river water quality management, where uncertainties associated with water quality parameters, cost functions, and environmental requirements were described as interval values. IQP was applied to a real-case study of the Xiangxihe River, China. A multisegment simulation model of one river with one tributary was used to generate water-quality transformation matrices and vectors, which were used to establish the river water quality constraints; in addition, the cost function of wastewater treatment were expressed as quadratic form. System cost, wastewater treatment efficiencies, and river pollution situations were analyzed under two scenarios and three criteria. A number of cost-effective schemes were generated by adjusting different combinations of decision variables within their solution intervals. Results can help decision makers generate alternatives between wastewater treatment cost and stream water quality requirement under uncertainty. [ABSTRACT FROM AUTHOR]

Seamounts are ubiquitous in the ocean. However, little is known about how seamount habitat features influence the local microbial community. In this study, the microbial populations of sediment cores from sampling depths of 0.1 to 35 cm from 10 seamount summit sites with a water depth of 1,850 to 3,827 m across the South China Sea (SCS) Basin were analyzed. Compared with nonseamount ecosystems, isolated seamounts function as oases for microbiomes, with average moderate to high levels of microbial abundance, richness, and diversity, and they harbor distinct microbial communities. The distinct characteristics of different seamounts provide a high level of habitat heterogeneity, resulting in the wide range of microbial community diversity observed across all seamounts. Using dormant thermospores as tracers to study the effect of dispersal by ocean currents, the observed distance-decay biogeography across different seamounts shaped simultaneously by the seamounts' naturally occurring heterogeneous habitat and the limitation of ocean current dispersal was found. We also established a framework that links initial community assembly with successional dynamics in seamounts. Seamounts provide resource-rich and dynamic environments, which leads to a dominance of stochasticity during initial community establishment in surface sediments. However, a progressive increase in deterministic environmental selection, correlated with resource depletion in subsurface sediments, leads to the selective growth of rare species of surface sediment communities in shaping the subsurface community. Overall, the study indicates that seamounts are a previously ignored oasis in the deep sea. This study also provides a case study for understanding the microbial ecology in globally widespread seamounts. IMPORTANCE Although there are approximately 25 million seamounts in the ocean, surprisingly little is known about seamount microbial ecology. We provide evidence that seamounts are island-like habitats harboring microbial communities distinct from those of nonseamount habitats, and they exhibit a distance-decay pattern. Environmental selection and dispersal limitation simultaneously shape the observed biogeography. Coupling empirical data with a null mode revealed a shift in the type and strength, which controls microbial community assembly and succession from the seamount surface to the subsurface sediments as follows: (i) community assembly is initially primarily driven by stochastic processes such as dispersal limitation, and (ii) changes in the subsurface environment progressively increase the importance of environmental selection. This case study contributes to the mechanistic understanding essential for a predictive microbial ecology of seamounts., Competing Interests: The authors declare no conflict of interest.

Panax notoginseng (Burk.) F. H. is a genuine medicinal material in Yunnan Province. As accessories, P. notoginseng leaves mainly contain protopanaxadiol saponins. The preliminary findings have indicated that P. notoginseng leaves contribute to its significant pharmacological effects and have been administrated to tranquilize and treat cancer and nerve injury. Saponins from P. notoginseng leaves were isolated and purified by different chromatographic methods, and the structures of 1 - 22 were elucidated mainly through comprehensive analyses of spectroscopic data. Moreover, the SH-SY5Y cells protection bioactivities of all isolated compounds were tested by establishing L-glutamate models for nerve cell injury. As a result, twenty-two saponins, including eight dammarane saponins, namely notoginsenosides SL 1 -SL 8 ( 1 - 8 ), were identified as new compounds, together with fourteen known compounds, namely notoginsenoside NL-A 3 ( 9 ), ginsenoside Rc ( 10 ), gypenoside IX ( 11 ), gypenoside XVII ( 12 ), notoginsenoside Fc ( 13 ), quinquenoside L 3 ( 14 ), notoginsenoside NL-B 1 ( 15 ), notoginsenoside NL-C 2 ( 16 ), notoginsenoside NL-H 2 ( 17 ), notoginsenoside NL-H 1 ( 18 ), vina-ginsenoside R 13 ( 19 ), ginsenoside II ( 20 ), majoroside F 4 ( 21 ), and notoginsenoside LK 4 ( 22 ). Among them, notoginsenoside SL 1 ( 1 ), notoginsenoside SL 3 ( 3 ), notoginsenoside NL-A 3 ( 9 ), and ginsenoside Rc ( 10 ) showed slight protective effects against L-glutamate-induced nerve cell injury (30 µM).

Importance: The strategic option between vascular supercharge and flap prefabrication in the fabrication of multipedicle flaps for difficult head and neck reconstruction has not been reported. Objectives: To summarize our 13-year experience in the design of multipedicle pre-expanded perforator flaps for extensive head and neck defect reconstruction, with vascular supercharge and flap prefabrication, either solely or in combination. To discuss the strategy for judicious selection between these techniques in the design of multipedicle flaps at different donor sites. Design, Setting, and Participants: A retrospective study was performed in patients with severe head and neck deformities and treated with multipedicle pre-expanded perforator flaps between May 2005 and May 2018. Intraoperative indocyanine green angiography was utilized for visualization of flap perfusion and analysis of the hemodynamics of pre-existing perforator and prefabricated vessels. Main Outcomes and Measures: The main outcomes measured were (1) features of deformities, (2) multipedicle flap designs, and (3) postoperative complications and long-term functional and aesthetic outcomes. Results: Seventy-five multipedicle pre-expanded perforator flaps were harvested, including 44 supercharged, 26 prefabricated, and 5 tripedicle, combining both techniques, with sizes ranging from 22 × 12 to 45 × 27 cm 2 . Hemodynamic analysis demonstrated slower arterial inflow (0.60 ± 0.29 U/s vs. 2.65 ± 1.29 U/s, p  < 0.05) and venous outflow (0.10 U/s vs. 0.23 ± 0.11 U/s) in prefabricated vessels, compared to the pre-existing perforator, namely the internal mammary arterial perforator. Partial necroses were observed in three patients with only one requiring skin grafting. The aesthetic and functional outcomes were satisfying after reconstruction. Conclusions and Relevance: Multipedicle pre-expanded perforator flaps fabricated by vascular supercharge and flap prefabrication, either solely or in combination in various donor sites, are powerful reconstructive tools for extensive head and neck defects. For a more reliable design, vascular supercharging should be considered a priority strategy, and flap prefabrication an alternative when suitable supercharging vessels are unavailable.

Using a culture-independent method that combines CARD-FISH, qPCR and 16S rDNA, we investigated the abundance, community structure and diversity of microbes along a steep thermal gradient (50-90 °C) in the Tengchong Geothermal Field. We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C). Under low-temperature conditions (52.3-74.6 °C), the microbial communities were dominated by Bacteria, which accounted for 60-80% of the total number of cells. At 74.6 °C, Archaea were dominant, and at 90.8 °C, they accounted for more than 90% of the total number of cells. Additionally, the microbial communities at high temperatures (74.6-90.8 °C) were substantially simpler than those at the low-temperature sites. Only a few genera (e.g., bacterial Caldisericum, Thermotoga and Thermoanaerobacter, archaeal Vulcanisaeta and Hyperthermus) often dominated in high-temperature environments. Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected. AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

Six new ellagitannins herein, rubusuaviins A-F, were isolated from the aqueous acetone extract of Chinese sweet tea (Tien-cha, dried leaves of Rubus suavissimus S. LEE) together with seven known tannins. Rubusuaviin A was characterized as 1-O-galloyl-2,3-O-(S)-HHDP-4,6-O-(S)-sanguisorboyl-beta-D-glucopyranose. Rubusuaviins B, C, and E are dimeric, trimeric, and tetrameric ellagitannins, respectively, in which the sanguisorboyl groups were connected ellagitannin units. Rubusuaviins D and F were desgalloyl derivatives of rubusuaviins C and E, respectively. The inhibition of alpha-amylase activity by rubusuaviins and related ellagitannins was compared. Ellagitannins with beta-galloyl groups at the glucose C-1 positions showed stronger inhibition compared with the alpha-galloyl and desgalloyl compounds. The molecular weight of these compounds was not important for the inhibition of alpha-amylase activity.