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Key Points: Question: Is dual antiplatelet therapy noninferior to intravenous thrombolysis in patients with minor nondisabling acute ischemic stroke? Findings: In this noninferiority randomized clinical trial that included 760 participants, excellent neurologic function at 90 days (modified Rankin Scale score of 0 or 1) occurred in 93.8% of those randomized to receive dual antiplatelet therapy and 91.4% of those randomized to receive intravenous alteplase, a difference greater than the prespecified noninferiority margin of −4.5%. Meaning: Among patients with minor nondisabling acute ischemic stroke, dual antiplatelet therapy, compared with intravenous alteplase, met the criteria for noninferiority with regard to excellent functional outcome at 90 days. Importance: Intravenous thrombolysis is increasingly used in patients with minor stroke, but its benefit in patients with minor nondisabling stroke is unknown. Objective: To investigate whether dual antiplatelet therapy (DAPT) is noninferior to intravenous thrombolysis among patients with minor nondisabling acute ischemic stroke. Design, Setting, and Participants: This multicenter, open-label, blinded end point, noninferiority randomized clinical trial included 760 patients with acute minor nondisabling stroke (National Institutes of Health Stroke Scale [NIHSS] score ≤5, with ≤1 point on the NIHSS in several key single-item scores; scale range, 0-42). The trial was conducted at 38 hospitals in China from October 2018 through April 2022. The final follow-up was on July 18, 2022. Interventions: Eligible patients were randomized within 4.5 hours of symptom onset to the DAPT group (n = 393), who received 300 mg of clopidogrel on the first day followed by 75 mg daily for 12 (±2) days, 100 mg of aspirin on the first day followed by 100 mg daily for 12 (±2) days, and guideline-based antiplatelet treatment until 90 days, or the alteplase group (n = 367), who received intravenous alteplase (0.9 mg/kg; maximum dose, 90 mg) followed by guideline-based antiplatelet treatment beginning 24 hours after receipt of alteplase. Main Outcomes and Measures: The primary end point was excellent functional outcome, defined as a modified Rankin Scale score of 0 or 1 (range, 0-6), at 90 days. The noninferiority of DAPT to alteplase was defined on the basis of a lower boundary of the 1-sided 97.5% CI of the risk difference greater than or equal to −4.5% (noninferiority margin) based on a full analysis set, which included all randomized participants with at least 1 efficacy evaluation, regardless of treatment group. The 90-day end points were assessed in a blinded manner. A safety end point was symptomatic intracerebral hemorrhage up to 90 days. Results: Among 760 eligible randomized patients (median [IQR] age, 64 [57-71] years; 223 [31.0%] women; median [IQR] NIHSS score, 2 [1-3]), 719 (94.6%) completed the trial. At 90 days, 93.8% of patients (346/369) in the DAPT group and 91.4% (320/350) in the alteplase group had an excellent functional outcome (risk difference, 2.3% [95% CI, −1.5% to 6.2%]; crude relative risk, 1.38 [95% CI, 0.81-2.32]). The unadjusted lower limit of the 1-sided 97.5% CI was −1.5%, which is larger than the −4.5% noninferiority margin (P for noninferiority <.001). Symptomatic intracerebral hemorrhage at 90 days occurred in 1 of 371 participants (0.3%) in the DAPT group and 3 of 351 (0.9%) in the alteplase group. Conclusions and Relevance: Among patients with minor nondisabling acute ischemic stroke presenting within 4.5 hours of symptom onset, DAPT was noninferior to intravenous alteplase with regard to excellent functional outcome at 90 days. Trial Registration: ClinicalTrials.gov Identifier: NCT03661411 This randomized trial examines whether dual antiplatelet therapy is noninferior to intravenous thrombolysis among patients with minor nondisabling acute ischemic stroke. [ABSTRACT FROM AUTHOR]

As the world's leading producer of farmed aquatic food products, China faces great uncertainty concerning the further sustainable development of its aquaculture industry due to high and increasing pressure from environmental and resource constraints. To realize truly sustainable development, it is imperative to establish an evaluation system that integrates social, economic, environmental and resource criteria, and optimizes aquaculture systems through a holistic approach. Here, we used analytical hierarchy processes combined with expert judgment and objective data to assess the sustainability of 10 major aquaculture production systems (APSs) in China. Based on the evaluation results, we propose the ecological intensification of aquaculture systems (ELIAS), rationally integrating anthropogenic inputs with aquaculture ecosystem services, to improve the sustainability of APSs. The holistic evaluation system and ELIAS are fundamental and critical for the future development of aquaculture in China and globally. [ABSTRACT FROM AUTHOR]

The cover image is based on the Review Article Optimization of aquaculture sustainability through ecological intensification in China by Shuang‐lin Dong et al., https://doi.org/10.1111/raq.12648 [ABSTRACT FROM AUTHOR]

Ambrosia artemisiifolia L. from Ambrosia of the Heliantheae of the Asteraceae family is a recognized harmful weed worldwide and one of the major invasive foreign plants in China. In this study, we investigated its reproductive features, focusing on its microsporogenesis, microgametogenesis, and pollen morphology. The results show that (1) Ambrosia artemisiifolia L. is a dicotyledonous plant and has spherical, tricolpate pollen grains with spiny outer wall; (2) its anther wall comprises four layers, namely epidermis, endothecium, middle layers, and amoeboid tapetum; (3) cytokinesis of microspore mother cells is successive; (4) most of tetrads are tetrahedral; and (5) mature pollen grains are three-celled. In conclusion, although Ambrosia artemisiifolia L. is a dicotyledonous plant with tricolpate pollen, its microsporogenesis is successive, which is different from typical dicots. [ABSTRACT FROM AUTHOR]

Biological soil crusts (biocrusts) are widely distributed in arid and semi-arid environments worldwide. The microbiome metabolic pathways of biocrusts contribute to important biogeochemical processes that improve the physicochemical properties of the surface soil. However, the differences in microbial processes among different types of biocrusts have been poorly studied by metagenomic analyses. In this study, we compared two types of biocrusts (bacterial-dominated biocrusts and moss-dominated biocrusts) using shotgun metagenomic sequencing. Our results showed that Actinobacteria was the most abundant phylum in the microbiomes of bacterial and moss biocrusts, even though the two biocrusts differed in the composition of the following other abundant phyla Proteobacteria , Acidobacteria , Cyanobacteria , Planctomycetes and Bacteroidetes. The profile of the C- and N- related metabolic pathways of the metagenome differed between the bacterial and moss biocrusts. The results showed that the genes encoding carbon monoxide dehydrogenase were more abundant than the gene encoding the ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) involved in the Calvin cycle, which is actually the main metabolic pathway for carbon fixation in both biocrusts. Low diversity in N 2 fixation pathways appears to be a distinguishing feature of biocrust microbiomes, while nitrogen reduction dominated the metabolic networks of the two biocrusts. The microbiome of bacterial biocrusts rich in assimilatory nitrate reduction genes, while the microbiome of moss biocrusts was rich in dissimilatory nitrate reduction genes. Amongst the (bio-)chemical parameters it is total organic carbon(TOC) that differentiates between the bacterial and moss biocrusts. Our study suggests that the biocrust types have significant effects on the TOC content and taxonomic, gene and metabolic diversity. • Different biocrusts can improve surface soil properties in arid environments. • The microbiomes of different biocrusts differ in contribution to biogeochemical processes. • Metagenomics provides a powerful tool for understanding the functional diversity of microbiomes. • The microbiomes of cynobacterial and mossy biocrusts differ taxonomically and functionally. • NO 3 − reduction seems to dominate the N cycle in biocrusts. [ABSTRACT FROM AUTHOR]

Andrographis paniculata is an important medicinal plant in the Lingnan region of China, which has the functions of clearing heat, removing toxins, and resisting bacteria and inflammation. The TCP gene family is a class of transcription factors that regulate plant growth, development, and stress response. In order to analysis the role of the TCP gene family under abiotic stress in A. paniculata, this study identified the TCP gene family of A. paniculata at the genome-wide level and analyzed its expression pattern in response to abiotic stress. The results showed that the A. paniculata TCP gene family had 23 members, with length of amino acid ranging from 136 to 508, the relative molecular mass between 14 854.71 and 55 944.90 kDa, and the isoelectric point between 5.67 and 10.39. All members were located in the nucleus and unevenly distributed on 13 chromosomes. Phylogenetic analysis classified them into three subfamilies: PCF, CIN and CYC/TB1. Gene structure and conserved motif analysis showed that most members of the TCP gene family contained motif 1, motif 2, motif 3 in the same order and 1-3 CDS. The analysis of promoter cis-acting elements showed that the transcriptional expression of the TCP gene family in A. paniculata might be induced by light, hormones, and adversity stress. In light of the expression pattern analysis and qRT-PCR verification, the expression of ApTCP4, ApTCP5, ApTCP6, and ApTCP11 involved in response by various abiotic stresses such as drought, high temperature, and MeJA. This study lays the foundation for in-depth exploration of the functions of A. paniculata TCP genes in response to abiotic stress.

Green tide in the sea is an abnormal ecological phenomenon caused by the explosive proliferation or high aggregation of some green macroalgae under particular environmental conditions. Since 2007, green tides have occurred in the Yellow Sea for 15 consecutive years, resulting in extremely serious damage to coastal ecological environment, social development, and economic construction. Therefore, it is urgent to solve the green tide problem, which is driven by external and internal factors. Global researchers have scientifically recognized the external causes (environmental factors) of the green tide blooms, and have carried out a series of studies on the physiological response of green tide algae to some environmental factors and obtained some achievements. However, the internal causes of green tide blooms, which is the intense ecophysiological adaptability of green tide algae in response to drastic fluctuation of environmental factors, has not yet been sufficiently addressed. From the perspective of algae ecophysiology, we reviewed the response mechanisms of green tide forming species to the fluctuations of various ecological factors, including light intensity, carbon dioxide, temperature, salinity, desiccation, nutrient, heavy metals, and biotic factors. Moreover, we summarized the adaptive regulation mechanisms of green tide algae dealing with fluctuating environmental factors from the aspects of photosynthetic and growth physiology, nutritional physio-logy, and reproductive physiology, which help reveal the internal mechanisms of green tide blooming.