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Floodplain wetlands are among the most dynamic ecosystems on Earth, featuring high biodiversity and productivity. They are also sensitive to anthropogenic disturbances and are globally threatened. Understanding how flow regime drives the spatiotemporal dynamics of wetland habitats is fundamental to effective conservation practices. In this study, using Landsat imagery and the random forest (RF) machine learning algorithm, we mapped the winter distribution of four wetland habitats (i.e., Carex meadow, reedbed, mudflat, and shallow water) in East Dongting Lake, a Ramsar wetland in the middle to lower Yangtze Basin of China, for 34 years (1988–2021). The dynamics of wetland habitats were explored through pixel-by-pixel comparisons. Further, the response of wetland habitats to flow regime variations was investigated using generalized additive mixed models (GAMM). Our results demonstrated the constant expansion of reedbeds and shrinkage of mudflats, and that there were three processes contributing to the reduction in mudflat: (1) permanent replacement by reedbed; (2) irreversible loss to water; and (3) transitional swapping with Carex meadow. These changes in the relative extent of wetland habitats may degrade the conservation function of the Ramsar wetland. Moreover, the duration of the dry season and the date of water level withdrawal were identified as the key flow regime parameters shaping the size of wetland habitats. However, different wetland vegetation showed distinct responses to variations in flow regime: while Carex meadow increased with earlier water withdrawal and a longer dry season, reedbed continuously expanded independent of the flow regime corresponding to the increase in winter rainfall. Our findings suggested that flow regime acts in concert with other factors, such as climate change and sand mining in river channels, driving wetland habitat transition in a floodplain landscape. Therefore, effective conservation can only be achieved through diverse restoration strategies addressing all drivers. [ABSTRACT FROM AUTHOR]

The Lesser White‐fronted Goose (Anser erythropus), smallest of the "gray" geese, is listed as Vulnerable on the IUCN Red List and protected in all range states. There are three populations, with the least studied being the Eastern population, shared between Russia and China. The extreme remoteness of breeding enclaves makes them largely inaccessible to researchers. As a substitute for visitation, remotely tracking birds from wintering grounds allows exploration of their summer range. Over a period of three years, and using highly accurate GPS tracking devices, eleven individuals of A. erythropus were tracked from the key wintering site of China, to summering, and staging sites in northeastern Russia. Data obtained from that tracking, bolstered by ground survey and literature records, were used to model the summer distribution of A. erythropus. Although earlier literature describes a patchy summer range, the model suggests a contiguous summer habitat range is possible, although observations to date cannot confirm A. erythropus is present throughout the modeled range. The most suitable habitats are located along the coasts of the Laptev Sea, primarily the Lena Delta, in the Yana‐Kolyma Lowland, and smaller lowlands of Chukotka with narrow riparian extensions upstream along major rivers such as the Lena, Indigirka, and Kolyma. The probability of A. erythropus presence is related to areas with altitude less than 500 m with abundant wetlands, especially riparian habitat, and a climate with precipitation of the warmest quarter around 55 mm and mean temperature around 14°C during June‐August. Human disturbance also affects site suitability, with a gradual decrease in species presence starting around 160 km from human settlements. Remote tracking of animal species can bridge the knowledge gap required for robust estimation of species distribution patterns in remote areas. Better knowledge of species' distribution is important in understanding the large‐scale ecological consequences of rapid global change and establishing conservation management strategies. [ABSTRACT FROM AUTHOR]

Mining-induced tremor is a dynamic phenomenon occurring during the fracturing or slipping of rock and coal mass in coal mines, and the tremor mechanism is one of the key problems in the field of rockburst. The Bayesian inversion method can more accurately analyse the source rupture type under the premise of considering waveform data and model errors. First, we added different noise levels to the waveform and determined filtering parameters based on the spectrum. The rationality of the Bayesian inversion was verified by observing the focal mechanism solution. Finally, a total of fourteen mining-induced tremors were selected, and the parameters of the moment tensor (MT), type of rupture, and fault plane solutions were obtained. The results demonstrate that (1) the tremor events in the roof are mainly tensile/shear failure, and compression failure mainly occurs in the coal seam. (2) Events on the floor are closely related to gob and mining activities. (3) The rupture form of the events near the tectonic region is more complex, and the fault plane solutions (strike, dip, and slip) were obtained by MT conversion. (4) The relation between rockbursts and mining-induced tremors is discussed by analysing events 1# and 14# that occurred in the Xinjulong coal mine. These research findings can provide a reference for the analysis of source parameters and rupture forms of mining-induced tremors. [ABSTRACT FROM AUTHOR]

This study conducts the mid-long term predictions and assesses the multi-year average offshore wind energy in the China seas for the next five years based on CMIP5 wind data, in hope of providing reference for the long term prediction of wind energy. Results show that (1) large areas with multi-year average wind power densities (WPD) greater than 350 W/m² are mainly found throughout the Ryukyu Islands and the Taiwan and Luzon Straits in the southeastern area of the Indo-China Peninsula. (2) From 2015-2019, the WPD in the Bohai Sea, Taiwan Strait and Beibu Gulf will be lower than the multi-year average level and thus will be similar to the multi-year average in the Yellow Sea and Gulf of Thailand but greater than the multi-year average in the East China Sea and South China Sea. (3) The predicted occurrences of exploitable wind speeds of the East China Sea and South China Sea are promising for the next five years, exceeding 60%. A value above 40% is predicted for the Yellow Sea, and a lower value is anticipated for the Bohai Sea. (4) The predicted occurrences of WPD > 50 W/m² are more promising for the next five years. [ABSTRACT FROM AUTHOR]

Anthropogenic habitat alternation is one of the leading causes of biodiversity loss, especially in freshwater ecosystems. Understanding the mechanisms of how habitat degradation drives changes in spatial patterns of species assemblages is fundamental to conservation science. We conducted an empirical study to investigate the species richness (α-diversity) and community composition (β-diversity) of benthopelagic biota along the gradient of human modification in West Dongting Lake, China. The study site is a large floodplain lake that has been subjected to long-term intensive human use. For a better understanding of how β-diversity responds to human disturbance, we also distinguished between two patterns of β-diversity, i.e., turnover and nestedness. We found that both α- and β-diversity of the benthopelagic assemblage decreased along the intensity gradient of human modification, indicating the strong homogenizing effect of anthropogenic habitat alternation. Our results also suggested that multiple processes, including environmental filtering, dispersal limitation, and biological interactions, were important in shaping the benthopelagic assemblage structure because spatial turnover other than nestedness dominated the total β-diversity at both the lake and habitat level. These findings support that habitat restoration by removing artificial banks and channels and increasing the landscape naturalness would be the key for this highly modified floodplain lake. [ABSTRACT FROM AUTHOR]

With the development of social media, more and more people use online tools to interact with others and to express their opinions and emotions. This study examined online expression on People's Daily's microblog site in response to a heavy rainfall in Wuhan, China during early July of 2016. We used a computer program to conduct a sentiment analysis to analyze 14 official posts on the site and 30,161 comments under these threads to investigate online expression of emotion in response to the heavy rainstorm. Results showed that online expression of emotion went through four stages, including emergence, peak, declination and extinction. More important, this cycle was repeated because of new development in the event. Additionally, both positive and negative emotions were present in the comments; however, unlike many previous studies, positive emotion was dominant in the comments. We also found that interaction among netizens influences emotional expression in the online discussions. Theoretical and practical implications of these results are discussed. [ABSTRACT FROM AUTHOR]

This paper demonstrates the impact of an endemic fossorial animal, plateau pika (Ochotona curzoniae), on soil properties and N2O flux at the Zoige Wetland. Pika burrow and control sites without disturbance by pika were selected to measure the soil water content, bulk density, soil organic matter (SOM), NH4-N content and NO3-N content in August 2012. N2O fluxes were measured with static opaque chambers at these sites in June and August 2012. Pika burrowing altered soil aeration by transferring deeper soil to the surface and by constructing underground burrows, which significantly increased bulk density, and reduced soil water content, SOM and NH4-N content at 0–10 cm and 10–20 cm soil depth. N2O flux had a significant correlation with bulk density, SOM and NH4-N content. Pika burrowing significantly influenced N2O flux by increasing N2O flux at the control site from near zero to 0.063±0.011 mg m-2 h-1. Our findings described how pika burrowing influences the soil traits and significantly increases the principal greenhouse gas N2O emission. As plateau pika was commonly considered as a pest, our findings give a novel clue to effectively manage populations of plateau pika on the Qinghai-Tibet Plateau from the perspective of greenhouse gas emission. [ABSTRACT FROM AUTHOR]

Better understanding of the dynamics of hydrological connectivity between river and floodplain is essential for the ecological integrity of river systems. In this study, we proposed a regime-switch modelling (RSM) framework, which integrates change point analysis with dynamic linear regression, to detect and date change points in linear regression, and to quantify the relative importance of natural variations and anthropogenic disturbances. The approach was applied to the long-term hydrological time series to investigate the evolution of river-floodplain relation in Dongting Lake in the last five decades, during which the Yangtze River system experienced unprecedented anthropogenic manipulations. Our results suggested that 1) there were five distinct regimes during which the influence of inflows and local climate on lake water level changed significantly. The detected change points were well corresponding to the major events occurred upon the Yangtze; 2) although the importance of inflows from the Yangtze was greater than that of the tributaries flows over the five regimes, the relative contribution gradually decreased from regime 1 to regime 5. The weakening of hydrological forcing from the Yangtze was mainly attributed to the reduction in channel capacity resulting from sedimentation in the outfalls and water level dropping caused by river bed scour in the mainstream; 3) the effects of local climate was much smaller than these of inflows; and 4) since the operation of The Three Gorges Dam in 2006, the river-floodplain relationship entered a new equilibrium in that all investigated variables changed synchronously in terms of direction and magnitude. The results from this study reveal the mechanisms underlying the alternated inundation regime in Dongting Lake. The identified change points, some of which have not been previously reported, will allow a reappraisal of the current dam and reservoir operation strategies not only for flood/drought risk management but also for the maintenance and restoration of the regional ecological integrity. [ABSTRACT FROM AUTHOR]

Three yeast strains, named as FHL-A, FHL-B, and FHL-C, were isolated from peach fruit surfaces collected from different regions in the North of China highly produced protease and were presented as single separate group in the genus Metschnikowia by sequence comparisons of 26S rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region. BLASTn alignments on NCBI showed that the similarity of 26S rRNA gene sequences of the three strains to all sequences of other yeasts accessed into the GenBank/EMBL/DDBJ and other database was very low (≦93%). The phylogenetic tree based on the D1/D2 region of 26S rRNA gene sequences revealed that three strains are most closely related to Metschnikowia koreensis KCTC 7828T (AF257272.1) (sequence similarity: 93.0%) and Metschnikowia reukaufii CBS9709 (AJ716113.1) (sequence similarity: 93.0%). However, the strains are distinguished from M. koreensis by its non-assimilation of galactose, ribitol, and D-xylose, and by its growth at 37 °C or in vitamin-free medium, and are notably different from M. reukaufii by its non-assimilation of galactose, D-xylose, D-arabinose, and D-ribose, and by its growth at 35 °C or in vitamin-free medium. The strain FHL-B formed asci in V8 juice sporulation medium for 3 weeks. Therefore, the name Metschnikowia persici is proposed for the novel species, with FHL-B (= CBS12815 = CFCC 3578T) as the type strain. [ABSTRACT FROM AUTHOR]

Peatlands contain large amount of carbon stock that is vulnerable to release into the atmosphere. Mostly because of human impact, the peatlands at Zoige Wetlands face severe degradation, and the groundwater table is now lower than before, which has increased the population of the plateau zokor, a burrowing rodent. However, the impact of these changes on ecosystem carbon flows has not been studied. To investigate how the plateau zokor and the groundwater level alter the ecosystem respiration of the Zoige peatlands, we sampled the CO2 flux of hummocks shaped by the zokors and compared it with the CO2 flux of undisturbed sites with different groundwater table levels. The soil organic carbon (SOC), soil water content (SWC) and soil temperature at 5 cm (T5) were measured. SOC showed no significant difference among the four sampling sites and did not correlate with the CO2 flux, while SWC was found to partly determine the CO2 flux. A linear equation could adequately describe the relationship between the natural logarithm of the ecosystem respiration and the soil temperature. It is demonstrated that descending groundwater table might accelerate ecosystem respiration and the CO2 flux from hummocks was higher than the CO2 flux from the control site in the non-growing season. With rising temperature, the CO2 flux from the control site accelerated faster than that from the hummocks. Our results show that ecosystem respiration was significantly lower from hummocks than at the control site in the growing season. The results on the impact of zokors on greenhouse gas emissions presented in this paper provide a useful reference to help properly manage not only this, but other litter-burrowing mammals at peatland sites. [ABSTRACT FROM AUTHOR]

Poyang Lake is the largest freshwater lake in China and is of global importance for the conservation of migratory waterbirds of the East Asian - Australasian Flyway. Recent dam construction on the Yangtze River and its tributaries for agriculture and hydroelectric power has affected the hydrological regimes in downstream lakes. The Three Gorges Dam changed the hydrological regime of downstream lakes by reducing wet season flooding and expanding water storage in the dry season., Despite the critical role of Poyang Lake in regional and global biodiversity conservation and the potential adverse ecological impacts of the Three Gorges Dam on downstream lakes, there have been few studies of the hydrological requirements of wintering waterbirds in the middle Yangtze floodplains., We assembled a predictor matrix including three hydrological variables (annual inflow, maximum water level in high water season or Max WL and minimum water level in low water season or Min WL) and two climatic variables (annual rainfall and biological cumulative temperature or Bio T). Using the predictor matrix and annual waterbird census, we built group-specific generalised additive models ( GAM) to investigate how waterbird population variations were related to climatic and hydrological factors in the Poyang Lake National Natural Reserve. We then used the modelled predictor-response curves to identify the optimal lake water levels for each waterbird group., The community-level model selected group and the group-varying-coefficient term of Bio T, Inflow and Max WL as explanatory variables. At group level, tuber eaters and sedge foragers responded positively to Bio T and Min WL. Seed eaters, invertebrate eaters and fish eaters responded positively to Inflow and negatively to Max WL and Min WL. Based on the modelled predictor-response curves, we propose the following optimal water level ranges for Poyang Lake wintering waterbird conservation: a) maximum high water season level should be less than 17.4 m; and b) minimum low water level should be between 8.2 m and 8.8 m. [ABSTRACT FROM AUTHOR]

By combining satellite tracking, land-cover extracted from Landsite 8 images, and the traditional stable isotope analysis, we studied the habitat selection and food preference of a vulnerable migratory waterbird, the White-naped Crane (Grus vipio), in one of its key stagging sites, the Shandian River Basin in the semi-arid northern China, to provide knowledge that is critical for its conservation in the Anthropocene. Our results showed that the White-naped Cranes used both uplands and natural wetlands in the stopover site. While the cranes used farmland and natural land cover equally as night-time roosting grounds, they spent most daytime foraging at farmlands. Despite the extensive usage of croplands as their foraging ground, the Bayesian mixing models based on stable isotopic analysis revealed that crop residues after harvesting, such as Maize (Zea mays) and Naked Oat (Avena chinensis), were only a small fraction of the White-naped Cranes' diet (∼19%), and their diet composited mainly natural plants, such as Allium ledebourianum , Potentilla anserina , and P. tanacetifoli. Moreover, more than 20% of the total wetlands in the region were modelled as home range of the cranes. On contrast, less than 10% of croplands and about 1% of the unused uplands were identified as home range. In addition, the entire core habitats were located in natural wetlands. Our findings demonstrated the importance of natural wetlands for the survival of the threatened crane. However, the satellite-derived land cover data showed that croplands increased rapidly in the last decade in this area, at the expense of natural wetlands. With the sharp decrease of White-naped Crane population in China, the conservation of stopover sites becomes imperative. Based on our analysis, we recommend the following management actions: conserving adequate natural wetland area, regulating anthropogenic pressures such as the use of herbicides, expanding the duration and extent of current conservation regulations, establishing a comprehensive monitoring program, and initiating basin-scale ecological restoration, for effective conservation of this threatened species. These integrated conservation strategies for migratory waterbirds are necessary, considering the rapid land-cover changes and agricultural expansion that have been occurring in East Asian-Australasian Flyway, especially in the semi-arid temperate zone. [ABSTRACT FROM AUTHOR]

In the last 15 years, the west population of white-naped crane (Antigone vipio) decreased dramatically despite the enhanced conservation actions in both breeding and wintering areas. Recent studies highlighted the importance of protecting the integrity of movement connectivity for migratory birds. Widespread and rapid landcover changes may exceed the adaptive capacity of migrants, leading to the collapse of migratory networks. In this study, using satellite tracking data, we modeled and characterized the migration routes of the white-naped crane at three spatial levels (core area, migratory corridor, and migratory path) based on the utilization distribution for two eras (1990s and 2010s) spanning 20 years. Our analysis demonstrated that the white-naped crane shifted its migratory route, which is supported by other lines of evidences. The widespread loss of wetlands, especially within the stopover sites, might have caused this behavioral adaptation. Moreover, our analysis indicated that the long-term sustainability of the new route is untested and likely to be questionable. Therefore, directing conservation effects to the new route might be insufficient for the long-term wellbeing of this threatened crane and large-scale wetland restorations in Bohai Bay, a critical stopover site in the East Asian-Australasian flyway, are of the utmost importance to the conservation of this species. [ABSTRACT FROM AUTHOR]

[Objective] This study was aimed to develop the Chinese herbal formula with dysentery arresting and antidiarrheal effect. [Method] The Chinese herbal formulation was used to carry out the clinical curative effect test and pharmacological test for ill livestock. [Results] Curative effect test showed that the formula had good effect on abdominal pain, diarrhea and fecal changes of livestock, and the cure rate reached up to 92%. Pharmacological experiments showed that the formula had significant inhibitory effect on the gastrointestinal motility of the tested animals, and the diarrhea arresting and dysentery treating effect of the formula was also extremely significant. In addition, the formula was safe for tested animals, proving to be reliable. [Conclusion] The formula has distinct curative effect on treating white scour of piglets, yellow scour of piglets, livestock gastroenteritis and lienteric diarrhea, and it can also be used in veterinary clinic, as well as be used as feed additive. [ABSTRACT FROM AUTHOR]

Large river floodplain systems (LRFS) are among the most diverse and dynamic ecosystems. Accurately monitoring the dynamics of LRFS over long time series is fundamental and essential for their sustainable development. However, challenges remain because the spatial distribution of LRFS is never static due to inter- and intra-annual changes in environmental conditions. In this study, we developed and tested a methodological framework to re-construct the long-term wetland dynamics in Dongting Lake, China, utilizing an unsupervised machine-learning algorithm (UMLA) on the basis of MODIS (Moderate Resolution Imaging Spectroradiometer) EVI (Enhanced Vegetation Index) time series. Our results showed that the UMLA achieved comparable performance to the time-consuming satellite image segmentation method with a Kappa coefficient of agreement greater than 0.75 and an overall accuracy over 85%. With the re-constructed annual wetland distribution maps, we found that 31.35% of wet meadows, one of most important ecological assets in the region, disappeared at an average rate of c.a. 1660 ha year−1 during the past two decades, which suggests that the Dongting Lake is losing its ecological function of providing wintering ground for migratory water birds, and remediation management actions are urgently required. We concluded that UMLA offers a fast and cost-efficient alternative to monitor ecological responses in a rapidly changing environment. [ABSTRACT FROM AUTHOR]

• Environmental drivers and characters of algal blooms in Dongting Lake were studied. • Algal bloom potential was low, but functional traits suggested toxic hazards. • NH 4 + was the key factor for phytoplankton biomass and toxigenicity. • Sensitivity of zooplankton to environmental factors inhibited phytoplankton control. • Warm winters may exacerbate algal bloom risk. Algal bloom (AB) risk assessment is critical for maintaining ecosystem health and human sustainability. Previous AB risk assessments have focused on the potential occurrence of ABs and related factors in the growing season, whereas their hazards, especially in the pre-growing season, have attracted less attention. Here, we performed a comprehensive AB risk assessment, including water trophic levels, phytoplankton biomass, functional trait-based assemblages, and related environmental factors, in the pre-growing season in Dongting Lake, China. Although mesotrophic water and low phytoplankton biomass suggested low AB potential, toxic taxa, which constituted 13.28% of the phytoplankton biomass, indicated non-negligible AB hazards. NH 4 + and water temperature were key factors affecting phytoplankton motility and toxicity. Our study establishes a new paradigm for quantitative AB risk assessment, including both potential AB occurrence and hazards. We emphasize the importance of phytoplankton functional traits for early AB warning and NH 4 + reduction for AB control in the pre-growing season. [Display omitted] [ABSTRACT FROM AUTHOR]

Conservation tillage is an important management practice to guarantee soil fertility in degraded Mollisols. It is still unclear, however, whether the improvement and stability of crop yield under conservation tillage can be sustainable with increasing soil fertility and reducing fertilizer-N application. Based on a long-term tillage experiment initiated in Lishu Conservation Tillage Research and Development Station by Chinese Academy of Sciences, we conducted a 15 N tracing field micro-plot experiment to investigate the effects of reducing nitrogen application on maize yield and fertilizer-N transformation under long-term conservation tillage agroecosystem. There were four treatments, including conventional ridge tillage (RT), no-tillage with 0% (NT0), 100% (NTS) maize straw mul-ching, and 20% reduced fertilizer-N plus 100% maize stover mulching (RNTS). The results showed that after a complete cultivation round, the average percentages of fertilizer N recovery in soil residues, crop usage, and gaseous loss were 34%, 50%, and 16%, respectively. Compared with conventional ridge tillage, no-tillage with maize straw mulching (NTS and RNTS) significantly increased the use efficiency of fertilizer N in current season by 10% to 14%. From the perspective of N sourcing analysis, the average percentage of fertilizer N absorbed by crop parts (including seeds, straws, roots, and cobs) to the total N uptake reached nearly 40%, indicating that soil N pool was the main source of N for crop uptakes. In comparison with conventional ridge tillage, conservation tillage significantly increased total N storage in 0-40 cm by reducing soil disturbance and increasing organic inputs, and thus ensured the expansion and efficiency increment of soil N pool in degraded Mollisols. Compared with conventional ridge tillage, NTS and RNTS treatments significantly increased the maize yield from 2016 to 2018. In all, by improving fertilizer nitrogen utilization efficiency and maintaining the continuous supply of soil nitrogen, long-term management of no-tillage with maize straw mulching could achieve a stable and increasing maize yield in three consecutive growing seasons and simultaneously reduce environmental risks derived by fertilizer-N losses, even under the condition of 20% reduction of fertilizer-N application, and thus actualize the sustainable development of agriculture in Mollisols of Northeast China.

Objective: Due to the defects in skin barrier function and immune response, burn patients who survive the acute phase of a burn injury are at a high risk of nosocomial infection (NI). The aim of this study is to evaluate the impacts of NI on length of stay (LOS) and hospital mortality in burn patients using a multistate model., Design and Setting: A retrospective observational study was conducted in burn unit and intensive care unit in the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China., Participants: Data were obtained from 1143 records of patients admitted with burn between 1 January 2013 and 31 December 2016., Methods: Risk factors for NIs were determined by binary logistic regression. The extended Cox model with time-varying covariates was used to determine the impact of NIs on hospital mortality, and cumulative incidence functions were calculated. Multiple linear regression analysis was applied to detect the variables associated with LOS. Using a multistate model, the extra LOS due to NI were determined., Results: 15.8% of total burn patients suffered from NIs and incidence density of NIs was 9.6 per 1000 patient-days. NIs significantly increased the rate of death (HR 4.266, 95% CI 2.218 to 8.208, p=0.000). The cumulative probability of death for patients with NI was greater that for those without NI. The extra LOS due to NIs was 17.68 days (95% CI 11.31 to 24.05)., Conclusions: Using appropriate statistical methods, the present study further illustrated that NIs were associated with the increased cumulative incidence of burn death and increased LOS in burn patients., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2018. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

The risk factors and precautions of inpatient suicide were explored. Thirty suicide victims were drawn from the adverse event reports of suicidal act during hospitalization in a general hospital from 2008 to 2014. Data were gathered from the focus group interviews of twelve nurses who had experienced inpatient suicide. The data were analyzed by using analytical technique based on grounded theory, and software QSR NVIVO8 was used to aid the collation of data. Three main themes of risk factors about inpatient suicide emerged from the analysis: individual value, social factors and environmental factors. The individual value was categorized into different groups such as sense of guilt, hopelessness and low self-esteem. Social factors included two aspects of negative life events and social support. Three themes of precautions about inpatient suicide appeared in this study: evaluation, nursing and information exchange. Evaluation was elaborated from both physical and psychological assessments. This finding extends existing work of risk factors and precautions about inpatient suicide and brings new knowledge about the reasons why inpatients commit suicide.

Brown rot caused by Monilinia spp. is among the most important postharvest diseases of commercially grown stone fruits, and application of antagonistic yeasts to control brown rot is one promising strategy alternative to chemical fungicides. In this research, new yeast strains were isolated and tested for their activity against peach brown rot caused by Monilinia fructicola. Three yeast strains were originally isolated from the surface of plums (cv Chinese Angelino) collected in the north of China. In artificially wounded inoculation tests, the yeast reduced the brown rot incidence to 20 %. The population of the yeast within inoculated wounds on peaches significantly increased at 25 °C from an initial level of 5.0×10(6) to 4.45×10(7) CFU per wound after 1 day. The antagonistic strains were belonging to a new species of the genus Candida by sequence comparisons of 26 S rDNA D1/D2 domain and internal transcribed spacer region. The strains are most closely related to C. asparagi, C. musae and C. fructus on the basis of the phylogenetic trees based on the D1/D2 region of 26S rDNA. However, the strains are notably different from C. asparagi, C. musae and C. fructus, in morphological and physiological characteristics. Therefore, the name Candida pruni is proposed for the novel species, with sp-Quan (=CBS12814T=KCTC 27526T=GCMC 6582T) as the type strain. Our study showed that Candida pruni is a novel yeast species with potential biocontrol against brown rot caused by M. fructicola on peaches.

By using phospholipid fatty acids (PLFA) method, this paper studied the soil microbial community structure in the soybean fields with different chlorimuron-ethyl application history in Weihe district of Heilongjiang Province. In the meantime, the residual amount of chlorimuron-ethyl in soil was determined. There was a very low residual of chlorimuron-ethyl in soil under the conditions of different chlorimuron-ethyl application history. With the increasing year of chlorimuron-ethyl application, the total concentration of soil microbial PLFA and the ratios of fungi/bacteria and Gram negative/Gram positive bacteria decreased, and the microbial stress level increased. Principal component analysis (PCA) showed that long-term application of chlorimuron-ethyl into soybean field changed the soil microbial community structure significantly.