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This study examined the predictive role of several learner factors in second language (L2) incidental vocabulary acquisition through reading: L2 proficiency, motivation, anxiety, and mastery of strategies. Participants were 129 English learners in a comprehensive university in China. Participants read two English texts and were given an unannounced vocabulary test to assess their incidental vocabulary acquisition. Their levels of motivation, anxiety, and mastery of strategies were measured by three specifically designed instruments that targeted incidental vocabulary acquisition. A multiple linear regression analysis revealed that L2 proficiency, anxiety, and mastery of strategies were positive and significant predictors of incidental vocabulary acquisition, although motivation was not. This confirms the role that learners' linguistic, affective, and cognitive features play in L2 incidental vocabulary acquisition.

Algae are believed to give health benefits. However, the studies showed they contain toxic elements, including radionuclides, and may affect human health. The study presents the values of activity concentrations of 210Po and 210Pb in the algae supplements available worldwide for adults. The activity concentrations (Bq/kg dw) ranged from 0.07 to 14.5 (210Po) and from 0.06 to 8.48 (210Pb). Also, the effective radiation doses and the cancer risk from 210Po and 210Pb decay ingested with analyzed algal supplements have been assessed. The highest values of annual effective doses have been estimated for 210Po in the recommended portion of Spirulina (Arthrospira platensis) from China (59.7 μSv/year) and Diatomaceous earth from the USA (50.4 μSv/year). The cancer morbidity and mortality risk ranged from 10−4 to 10−8. The study indicated the activity concentrations of 210Po and 210Pb were low, and algae supplements for human consumption could be considered safe food. [Display omitted] • 210Po and 210Pb are present in algae supplements • Wild-growing Ch. vulgaris contained more 210Po and 210Po than aquafarmed • The highest annual doses from 210Po decay would come from Chinese A. platensis and diatomite from the USA • Algal superfoods can be considered safe [ABSTRACT FROM AUTHOR]

Background: Culture-based diagnostic methods cannot achieve rapid and precise diagnoses for the identification of multiple diarrhoeal pathogens (DPs). A high-throughput multiplex genetic detection system (HMGS) was adapted and evaluated for the simultaneous identification and differentiation of infectious DPs and a broad analysis of DP infection aetiology. Results: DP-HMGS was highly sensitive and specific for DP detection compared with culture-based techniques and was similar to singleplex real-time PCR. The uniform level of sensitivity of DP-HMGS for all DPs allowed us to remap the aetiology of acute diarrhoeal infections in Shanghai, correcting incidences of massively underdiagnosed DP species with accuracy approaching that of sequencing-based methods. The most frequent DPs were enteropathogenic Escherichia coli, rotavirus and Campylobacter jejuni. DP-HMGS detected two additional causes of infectious diarrhoea that were previously missed by routine culture-based methods: enterohemorrhagic E. coli and Yersinia enterocolitica. We demonstrated the age dependence of specific DP distributions, especially the distributions of rotavirus, intestinal adenovirus and Clostridium difficile in paediatric patients as well as those of dominant bacterial infections in adults, with a distinct “top 3” pattern for each age group. Finally, the multiplexing capability and high sensitivity of DP-HMGS allowed the detection of infections co-induced by multiple pathogens (approximately 1/3 of the cases), with some DPs preferentially co-occurring as infectious agents. Conclusions: DP-HMGS has been shown to be a rapid, specific, sensitive and appropriate method for the simultaneous screening/detection of polymicrobial DP infections in faecal specimens. Widespread use of DP-HMGS is likely to advance routine diagnostic and clinical studies on the aetiology of acute diarrhoea. [ABSTRACT FROM AUTHOR]

Severe Helicobacter pylori -linked gastric disorders are especially prevalent in the East Asia region. The ability of H. pylori to cause different clinical outcomes is thought to be associated with unique sets of its genetic features. However, only few genetic features have been definitively linked to specific gastrointestinal pathologies. Genome heterogeneity of clinical H. pylori strains from patients with four different gastric disorders was studied to explore the population structure and molecular genomic features and their association with pathogenicity. Population analysis showed that 92.9% of the Shanghai H. pylori isolates were clustered in the East Asia group. Among 2,866 genes detected in all genomes, 1,146 genes formed the core genome, whereas 209 unique genes were detected in individual disease groups. The unique genes of peptic ulcer and gastric cancer groups represented the inorganic ion transport and metabolism function gene clusters. Sixteen virulence genes were detected with statistically different detection rates among the four disease groups. Furthermore, 127 clustered regularly interspaced short palindromic repeats were found with significantly different rates in the four disease groups. A total of 337 putative genomic islands were identified, and three genomic islands were individually found in more than 10% of strains. The genomic islands included several metabolism-associated genes and many genes with unknown function. In total, 88 sequence types were detected among the 112 Shanghai H. pylori isolates. Our study provides an essential milestone in the mapping of specific genomic features and their functions to identify factors needed to induce specific gastric disorders in H. pylori.

This pioneering study aimed to determine the activity concentrations of 210 Po, 210 Pb and uranium ( 234 U, 235 U, 238 U) radionuclides in fruit bodies of wild bolete Boletus bainiugan Dentinger and to estimate its edible safety, which may give scientific evidence for the consumption of this species. The analyses were performed using alpha spectrometer after digestion, exchange resins separation and deposition. Measurement data were analysed and interpolation maps reflecting 210 Po, 210 Pb and uranium ( 234 U, 235 U, 238 U) geographical distribution in Yunnan province (China) were presented. In addition, from the perspective of food safety, the possible related effective radiation dose to mushrooms consumers were estimated. The results indicated that 210 Po, 210 Pb and uranium ( 234 U, 235 U, 238 U) radionuclides contents in B. bainiugan were significantly different with respect to geographical distribution, and their possible intake in a part of the region was considerably higher. A very interesting observation was done according to the values of 235 U/ 238 U activity ratio indicating the occurrence of uranium faction from the global fallout of nuclear weapon tests., (Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Aim: We established a high-throughput multiplex genetic detection system (HMGS) for identification of Helicobacter pylori with concomitant analysis of virulence and drug resistance., Materials & Methods: Confirmed 132 H. pylori cultures from gastric biopsies were screened by 20-gene site-HMGS, sequencing and E-test., Results: HMGS was highly sensitive and specific for H. pylori identification. Concordance rate between HMGS and sequencing averaged 94.5% (virulence genes) and 97.3% (resistance genes). Observed resistance rates to four mainstream antibiotics were high, except for amoxicillin. Significant association between virulence genotype and risks for specific gastrointestinal diseases was found for five genes. Metronidazole resistance in peptic ulcer patients was significantly higher., Conclusion: HMGS is an effective method for H. pylori identification and analysis of virulence and drug resistance.