1. LncRNA EGOT decreases breast cancer cell viability and migration via inactivation of the Hedgehog pathway.
- Author
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Qiu S, Chen G, Peng J, Liu J, Chen J, Wang J, Li L, and Yang K
- Subjects
- Adult, Breast Neoplasms genetics, Carrier Proteins genetics, Carrier Proteins metabolism, Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Cell Survival genetics, China, Female, Gene Expression genetics, Gene Expression Regulation, Neoplastic genetics, Humans, Membrane Glycoproteins genetics, Membrane Glycoproteins metabolism, Middle Aged, Patched-1 Receptor genetics, Patched-1 Receptor metabolism, RNA, Long Noncoding metabolism, Signal Transduction genetics, Zinc Finger Protein GLI1 genetics, Zinc Finger Protein GLI1 metabolism, Breast Neoplasms metabolism, Hedgehog Proteins metabolism, RNA, Long Noncoding genetics
- Abstract
The long noncoding RNA (lncRNA) Eosinophil Granule Ontogeny Transcript (EGOT) has been reported to inhibit the proliferation and migration of glioma cells, and promote the development and progression of gastric cancer through the Hedgehog (Hh) signaling pathway. This study was conducted to assess the role of EGOT in the progression of breast cancer. We observed that EGOT is significantly down-regulated in breast cancer tissues and cell lines, and EGOT expression is negatively correlated with the Ki67 expression. Overexpression of EGOT in BT549 cells decreased cell viability and migration. In addition, overexpression of EGOT resulted in decreases in expression of key genes in the Hh pathway, including Gli1, smoothened protein, protein patched homolog 1 and Hedgehog-interacting protein (HHIP). Breast cancer tissues exhibited an increase in Gli1 expressions. Altered expression of Gli1, smoothened protein, protein patched homolog 1 and HHIP caused by EGOT overexpression were fully restored in cells transfected with plasmid complementory DNA (pcDNA) EGOT and treated with purmorphamine, an agonist of the Hh pathway. Cell viability and migration were also restored by purmorphamine. We conclude that lncRNA EGOT may inhibit breast cancer cell viability and migration via inactivation of the Hh pathway., (© 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.)
- Published
- 2020
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