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The wide use of rare-earth elements in China for aquacultural purposes and many other applications has resulted in their accumulation in the aquatic environment and has caused concern about their safety. In this study, we tested the toxicity of lanthanum (La (III)) to the early life stages (embryonic and sac-fry stages) of the rare minnow Gobiocypris rarus Ye & Fu, 1983. We exposed fertilized eggs to 0, 0.06, 0.13, 0.25, 0.50, 1.00, and 1.92 mg/L of La (III) until the yolk sac was exhausted in any group (at about 168 h of exposure). Exposure to 1.00 and 1.92 mg/L La (III) had obvious lethal effects on embryos, La (III) exposure also accelerated the development of embryos and had a significant inhibitory effect on the hatching rate after 96 h. As the exposure time increased, the larvae exhibited obvious yolk-sac edema, pericardium edema, spinal curvature, tail bending, and other symptoms of poisoning, including deflated swim-bladder. In general, these results clearly indicate that La pollutants hinder the development of rare minnow embryos and are acutely toxic to rare minnow larvae. Our finding would provide a theoretical basis for further research of relevant feed additive criteria for this fish. [ABSTRACT FROM AUTHOR]

Background: Adolescent idiopathic scoliosis (AIS) is the most common form of scoliosis. However, the underlying mechanisms linking spinal curvature in AIS to foot characteristics and walking performance remain unclear. Objective: This study aimed to compare walking performance between adolescents with mild, moderate, and severe scoliosis and matched healthy peers with foot posture as covariates. Methods: This cross-sectional study of 96 adolescents was conducted between April 2020 to October 2020 in China, with 32 healthy peers in the control group and 64 patients in the AIS group. Foot posture and morphology, plantar pressure distribution, and gait characteristics were analyzed. One-way analysis of variance with Bonferroni correction and a post hoc comparison of the mean differences between the different groups was performed. Multiple analyses of covariance adjusted for age, sex, body mass index, foot posture index (FPI), arch index (AI), and walking speed were performed. Results: Of the 64 adolescents with scoliosis, 18 had mild AIS, 32 had moderate AIS, and 14 had severe AIS. The AI and FPI were much higher in the moderate and severe AIS groups (p = 0.018) and the severe AIS group (p<0.001), respectively, than in the control group. The severe AIS group had advanced and longer midstance (p = 0.014) and delayed propulsion phase (p = 0.013) than the control group. Patients with moderate and severe AIS had asymmetrical gait periods in the left and right limbs (p<0.05). Significant differences in the center-of-pressure excursion index (CPEI) were found between the moderate and severe AIS and control groups (p = 0.003). Conclusion: Moderate and severe AIS significantly influenced walking performance; however, no significant differences were observed between adolescents with mild AIS and healthy controls. Thus, early intervention could target the prevention of specific functional deficits and prevent it from progressing to a severe state. [ABSTRACT FROM AUTHOR]

Olaquindox (OLA) has been widely used as an animal feed additive in China for decades; however, its toxicity and toxic mechanisms have not been well investigated. In this study, the developmental neurotoxicity and toxic mechanisms of OLA were evaluated in zebrafish. Zebrafish embryos were exposed to different concentrations of OLA (25–1,000 mg/L) from 6 to 120 hours post fertilization (hpf). OLA exposure resulted in many abnormal phenotypes in zebrafish, including shortened body length, notochord degeneration, spinal curvature, brain apoptosis, damage of axon and peripheral motor neuron, and hepatotoxicity. Interestingly, OLA increased zebrafish spontaneous tail coiling, while reduced locomotor capacity. Quantitative polymerase chain reaction (Q‐PCR) showed that the expression levels of nine marker genes for nervous system functions or development, namely, α1‐tubulin, glial fibrillary acidic protein (gfap), myelin basic protein (mbp), synapsinII a (syn2a), sonic hedgehog a (shha), encoding HuC (elavl3), mesencephalic astrocyte‐derived neurotrophic factor (manf) growth associated protein 43 (gap43), and acetylcholinesterase (ache) were all down‐regulated significantly in zebrafish after treated with OLA. Besides, the anti‐apoptotic and pro‐apoptotic genes bcl‐2/bax ratio was reduced. These results show that OLA exposure could cause severe developmental neurotoxicity in the early stages of zebrafish life and OLA might induce neurotoxicity by inhibiting the expression of neuro‐developmental genes and promoting apoptosis. OLA exposure resulted in many abnormal phenotypes in zebrafish, including shortened body length, notochord degeneration, spinal curvature, brain apoptosis, damage of axon and peripheral motor neuron and hepatotoxicity. OLA increased zebrafish spontaneous tail coiling, while reduced locomotor capacity. Q‐PCR showed that the expression of nine marker genes for nervous system functions or development were all down‐regulated significantly, and the apoptotic bcl‐2/bax gene expression ratio was reduced. These results show that OLA exposure could cause developmental neurotoxicity in the early stages of zebrafish life for the first time. [ABSTRACT FROM AUTHOR]

Purpose: To investigate the age-based normal values of sagittal parameters and establish the relationships between them in Chinese population.Method: Two hundred eighteen asymptomatic adult volunteers were included in this cross-sectional study. The whole spine standing radiograph was taken from them, and the parameters including sagittal vertical axis (SVA), T1 pelvic angle (TPA), global tilt (GT), spino-sacral angle, lumbar lordosis (LL), thoracic kyphosis (TK), T1 slope (T1S), cervical lordosis (CL), C2-C7SVA, pelvic tilt (PT), sacral slop (SS) and pelvic incidence (PI) were measured. The gender differences in sagittal alignment were compared. Pearson correlation was calculated, and a linear regression analysis was used to establish the relation between PI and other parameters.Results: The average values of PI, LL, TPA and GT were 46.2°, 48.2°, 7.8° and 10.6°, respectively, in this cohort. SVA, GT, TPA, TK, T1S, CL and PT significantly increased with age (p < 0.05). The females presented smaller T1S, C2-C7SVA and larger PI, PT than the males. The relationships between PI and TPA, GT, SS, LL could be presented as TPA = 0.411 * PI - 11.2 (R2 = 0.328, p < 0.001), GT = 0.483 * PI - 11.7 (R2 = 0.297, p < 0.001), SS = 0.354 * PI + 16.1 (R2 = 0.203, p < 0.001), LL = 0.588 * PI + 21.0 (R2 = 0.267, p < 0.001), respectively.Conclusion: The normal values of sagittal parameters were presented and changed with age in Chinese asymptomatic population. The gender differences existed in sagittal parameters. The relationships between PI and other parameters were established which could be used for further research. These slides can be retrieved under Electronic Supplementary Material. [ABSTRACT FROM AUTHOR]

Study Design: A prospective study.Objective: To investigate the incidence and risk factors of acute pancreatitis after scoliosis surgery.Summary Of Background Data: Pancreatitis has been recognized as a possible complication of extra-abdominal surgeries. However, there were few reports on the incidence and risk factors of acute pancreatitis after scoliosis surgery.Methods: A prospective clinical study was performed at our center from September 2014 to October 2015. One hundred seventy-six patients undergoing posterior spinal correction surgery were enrolled. The diagnosis of acute pancreatitis was based on their clinical manifestations combined with laboratory examination. Demographic, intraoperative, and radiological parameters were evaluated to identify the risk factors of acute pancreatitis after scoliosis surgery.Results: Thirteen patients (7.4%) were diagnosed with acute pancreatitis. Compared with patients without pancreatitis, pancreatitis patients had lower body mass index (BMI) (15.5 vs. 19.5, P = 0.001), larger preoperative Cobb angle of major curve (87.5° vs. 59.2°, P < 0.001), lower correction rate (57.4% vs. 69.0%, P = 0.045), lower intraoperative mean arterial pressure (57.9 mmHg vs. 66.1 mmHg, P < 0.001), and longer fusion levels (13.3 vs. 10.1, P < 0.001). No significant differences were noted with respect to operation time, intraoperative blood loss, or the amount of sagittal profile correction. Furthermore, multivariate logistic analysis revealed that BMI (odds ratio [OR] = 1.542, P = 0.009), lowest intraoperative mean arterial pressure (OR = 1.126, P = 0.039), and segments of fusion (OR = 0.551, P = 0.025) were independent risk factors for postoperative acute pancreatitis. All cases were treated with bowel rest, intravenous fluids, and fasting and completely recovered with 3.3 (2-5) days.Conclusion: The incidence of postoperative acute pancreatitis after scoliosis surgery was 7.4%. The low BMI, lowest intraoperative mean arterial pressure, and long segments of fusion were independent risk factors for acute pancreatitis after scoliosis surgery.Level Of Evidence: 3. [ABSTRACT FROM AUTHOR]

Study Design: A prospective randomized controlled trial.Objective: The aim of this study was to investigate the effectiveness of orthotic management versus exercise on spinal curvature, body symmetry, and quality of life.Summary Of Background Data: A number of well-designed studies comparing conservative treatment of adolescent idiopathic scoliosis (AIS) have been conducted and the evidence becomes stronger. However, there is a lack of the information on the effectiveness of orthotic management versus exercise.Methods: The inclusion criteria recommended by the Scoliosis Research Society (SRS) and the international Society on Scoliosis Orthopedic and Rehabilitation Treatment (SOSORT) were used during enrollment. Eligible patients were randomly assigned to either bracing group or exercise group. Patients in the bracing group were prescribed with a rigid thoracolumbosacral orthosis and requested to wear 23 h/day, while patients in the exercise group were treated with the protocol of the Scientific Exercise Approach to Scoliosis. Data regarding angle of trunk inclination, Cobb angle, shoulder balance, body image, and quality of life (QoL) were collected every 6 months.Results: Twenty-four patients in the bracing group and 29 patients in the exercise group participated in this study. For the intergroup comparison, the bracing group showed better results about the correction of spinal curvature (Cobb angle at the first 12 months of intervention, P = 0.039), scores concerning QoL, especially function (P < 0.001), mental health (P < 0.001), and total score (P < 0.001), were higher than that of the exercise group. The results of body symmetry evaluation did not differ significantly between the two groups. For the intragroup comparison, parameters of spinal curvature (baseline vs. 12-month, P < 0.03 in the exercise group and P < 0.001 in the bracing group), QoL (baseline vs. 12-month, P < 0.001), and TAPS (baseline vs. 12-month, P < 0.033) significantly improved over the studied period. Shoulder balance (baseline vs. 12-month, P < 0.005) showed significant improvement only in the bracing group.Conclusion: Both interventions of bracing and exercise showed significant treatment effectiveness on the patients with AIS. Bracing was superior to capture corrections in parameters of spinal curvature and body symmetry, while the QoL, especially in aspect of the functional and psychological status, was significantly better in the exercise group.Level Of Evidence: 1. [ABSTRACT FROM AUTHOR]

• Mortality and hatching rates significantly increased in medaka embryos exposed to OA. • The 96 h LC 50 of OA for 1-month old medaka larvae was calculated at 3.80 μg/mL. • Differentially expressed genes were enriched in 11 KEGG pathways with Q value < 0.05. • Cell division and proliferation, and nervous system functions were affected by OA. • Occurrence risks of cancer and major depressive disorder in medaka were suggested. The lipophilic okadaic acid (OA)-group toxins produced by some species of Dinophysis spp. and Prorocentrum spp. marine dinoflagellates have been frequently and widely detected in natural seawater environments, e.g. 2.1∼1780 ng/L in Spanish sea and 5.63∼27.29 ng/L in the Yellow Sea of China. The toxicological effects of these toxins dissolved in seawater on marine fish is still unclear. Effects of OA on the embryonic development and 1-month old larvae of marine medaka (Oryzias melastigma) were explored and discussed in this study. Significantly increased mortality and decreased hatching rates occurred for the medaka embryos exposed to OA at 1.0 μg/mL. Diverse malformations including spinal curvature, dysplasia and tail curvature were also observed in the embryos exposed to OA and the heart rates significantly increased at 11 d post fertilization. The 96 h LC 50 of OA for 1-month old larvae was calculated at 3.80 μg/mL. The reactive oxygen species (ROS) was significantly accumulated in medaka larvae. Catalase (CAT) enzyme activity was significantly increased in 1-month old larvae. Acetylcholinesterase (AChE) activity significantly increased with a dose-dependent pattern in 1-month old larvae. Differentially expressed genes (DEGs) were enriched in 11 KEGG pathways with Q value < 0.05 in 1-month old medaka larvae exposed to OA at 0.38 μg/mL for 96 h, which were mainly related to cell division and proliferation, and nervous system. Most of DEGs involved in DNA replication, cell cycle, nucleotide excision repair, oocyte meiosis, and mismatch repair pathways were significantly up-regulated, while most of DEGs involved in synaptic vesicle cycle, glutamatergic synapse, and long-term potentiation pathways were markedly down-regulated. This transcriptome analysis demonstrated that a risk of cancer developing was possibly caused by OA due to DNA damage in marine medaka larvae. In addition, the neurotoxicity of OA was also testified for marine fish, which potentially cause major depressive disorder (MDD) via the up-regulated expression of NOS1 gene. The genotoxicity and neurotoxicity of OA to marine fish should be paid attention to and explored further in the future. [ABSTRACT FROM AUTHOR]

Background: Retrospective analysis of the characteristics and correlation of spino-pelvic sagittal parameters in elderly patients with lumbar degenerative disease. Methods: Eighty-seven patients with lumbar degenerative disease, with an average age of 75.4 years old, were enrolled in the observation group. They were all from the orthopedics department of Beijing Hospital and got enrolled in this study from August 2015 to October 2017. Another 80 volunteers, with an average age of 74.5 years old, were enrolled in the control group. Standing lateral radiographs of the full-length spine were taken for all subjects. The following spino-pelvic sagittal parameters were measured: sagittal vertical axis (SVA), thoracic kyphosis (TK), lumbar lordosis (LL), pelvic incidence (PI), sacral slope (SS), and pelvic tilt (PT). Statistical analysis was performed to compare the differences of the parameters between groups, and the correlation analysis was also performed. Results: SVA, PI, and PT of the observation group were significantly higher than those of the control group (p < 0.01), while LL and SS were significantly lower in the observation group (p < 0.01). No significant differences were found in TK between the two groups. The correlation analyses showed that PI was significantly correlated with SS and PT in both the observation group (p < 0.01) and the control group (p < 0.01), so as the SVA-PI (p < 0.05) and SVA-PT (p < 0.01). SS-PT was also significantly correlated in the observation group (p < 0.01) and in the control group (p < 0.05). LL was significantly correlated with all the other parameters in the observation and control groups, including SVA (p < 0.01; p < 0.01), TK (p < 0.01; p < 0.01), PI (p < 0.01; p < 0.01), SS (p < 0.01; p < 0.01), and PT (p < 0.01; p < 0.01). SVA-SS (p < 0.05), TK-PI (p < 0.05), and TK-SS (p < 0.01) were significantly correlated in the control group but not in the observation group. Conclusion: Reduced coordination of the spine and pelvis in elderly patients with lumbar degenerative disease was observed. Many of the cases were in the state of sagittal imbalance, with the trunk center of gravity moving forward, the integral sagittal alignment becoming straight, and the pelvic posterior tilt increasing. Pelvic parameters were significantly correlated with each other, which may affect the sagittal curve of the spine. LL was a core parameter that significantly correlated with various sagittal parameters. [ABSTRACT FROM AUTHOR]