1. Antinociceptive mechanisms of dipsacus saponin C administered intrathecally in mice.
- Author
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Suh H, Song D, Huh S, Son K, and Kim Y
- Subjects
- Adrenergic alpha-Antagonists administration & dosage, Adrenergic alpha-Antagonists pharmacology, Analgesics administration & dosage, Animals, China, Dose-Response Relationship, Drug, GABA-A Receptor Antagonists, GABA-B Receptor Antagonists, Injections, Spinal, Male, Mice, Mice, Inbred ICR, Pain Measurement drug effects, Receptors, Adrenergic, alpha-2 drug effects, Receptors, Cholecystokinin drug effects, Receptors, Cholecystokinin metabolism, Receptors, GABA drug effects, Receptors, Serotonin drug effects, Saponins administration & dosage, Serotonin Antagonists pharmacology, Analgesics pharmacology, Oleanolic Acid analogs & derivatives, Plants, Medicinal chemistry, Saponins pharmacology
- Abstract
Dipsacus saponin C (DSC) administered intrathecally (i.t.) showed antinociceptive effect in a dose-dependent (from 3.75 to 30 microg) manner as measured by the tail-flick assay. The antinociception induced by DSC at the dose of 30 microg reached at peak 7.5 min and almost returned to the control level after 60 min. 5-Amino-valeric acid (5-AVA, a GABA(A) receptor antagonist, from 1 to 20 microg) and SR 95531 (a GABA(B) receptor antagonist, from 0.1 to 2 ng) dose-dependently attenuated i.t. administered DSC-induced increase of the inhibition of the tail-flick response. The i.t. injection of yohimbine (an alpha(2)-adrenergic receptor antagonist, from 1 to 20 microg) and methysergide (a serotonin receptor antagonist, from 1 to 20 microg), but not naloxone (from 2 to 8 microg), significantly attenuated inhibition of the tail-flick response induced by DSC (30 microg) administered i.t. Sulfated cholecystokinin (CCK, from 0.05 to 0.5 ng) injected i.t. significantly reduced the inhibition of the tail-flick response induced by DSC (30 microg) administered i.t. Our results suggest that DSC shows an antinociceptive effect when it is administered spinally and GABA(A), GABA(B), alpha(2)-adrenergic and serotonin receptors located at the spinal cord level, but not opioid receptors, may be involved in DSC-induced antinociception. Furthermore, CCK may play an important role for the modulation of i. t. injected DSC-induced antinociception.
- Published
- 2000
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