1. A validation study of a multiplex INDEL assay for forensic use in four Chinese populations.
- Author
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Wei, Yi-Liang, Qin, Cui-Jiao, Dong, Hui, Jia, Jing, and Li, Cai-Xia
- Subjects
FORENSIC genetics ,POPULATION genetics ,BIOLOGICAL assay ,DELETION mutation ,INSERTION mutation ,PERFORMANCE evaluation - Abstract
Insertion/deletion (INDELs) marker sets can serve as a useful supplementary tool for human identification. A commercial kit, the Qiagen DIPplex
® Investigator kit, multiplexes 30 biallelic autosomal INDELs and Amelogenin for forensic use. We performed a validation study based on the DIPplex® kit in four Chinese populations: Han, Tibetan, Uyghur, and Kazakh. There were no significant departures from Hardy–Weinberg equilibrium or significant linkage disequilibrium (pair-wised r2 <0.2) between the 30 INDELs. The random match probabilities were in the range of 3.84×10−11 to 1.20×10−12 , and the power of exclusion was >0.99. The multiplex PCR was optimized for a 5-μL volume, full profiles were obtained with 0.062ng/μL of template DNA, and excellent performance was obtained with degraded casework samples. This study demonstrates that the multiplex INDEL assay can be used as a supplementary method for degraded DNA detection in the studied Chinese populations. [ABSTRACT FROM AUTHOR]- Published
- 2014
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