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Detection of cotton bolls in the field environments is one of crucial techniques for many precision agriculture applications, including yield estimation, disease and pest recognition and automatic harvesting. Because of the complex conditions, such as different growth periods and occlusion among leaves and bolls, detection in the field environments is a task with considerable challenges. Despite this, the development of deep learning technologies have shown great potential to effectively solve this task. In this work, we propose an Improved YOLOv5 network to detect unopened cotton bolls in the field accurately and with lower cost, which combines DenseNet, attention mechanism and Bi-FPN. Besides, we modify the architecture of the network to get larger feature maps from shallower network layers to enhance the ability of detecting bolls due to the size of cotton boll is generally small. We collect image data of cotton in Aodu Farm in Xinjiang Province, China and establish a dataset containing 616 high-resolution images. The experiment results show that the proposed method is superior to the original YOLOv5 model and other methods such as YOLOv3,SSD and FasterRCNN considering the detection accuracy, computational cost, model size and speed at the same time. The detection of cotton boll can be further applied for different purposes such as yield prediction and identification of diseases and pests in earlier stage which can effectively help farmers take effective approaches in time and reduce the crop losses and therefore increase production. [ABSTRACT FROM AUTHOR]