11 results on '"Yang, Xing-Lou"'
Search Results
2. Identification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China.
- Author
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Tang Y, Zhao K, Yin HM, Yang LP, Wu YC, Li FY, Yang Z, Lu HX, Wang B, Yang Y, Zhang YZ, and Yang XL
- Subjects
- Animals, China epidemiology, RNA, Viral genetics, Genomics methods, High-Throughput Nucleotide Sequencing, Picornaviridae Infections veterinary, Picornaviridae Infections virology, Picornaviridae Infections epidemiology, Shrews virology, Phylogeny, Genome, Viral
- Abstract
Hepatitis A virus (HAV), a member of the genus Hepatovirus ( Picornaviridae HepV), remains a significant viral pathogen, frequently causing enterically transmitted hepatitis worldwide. In this study, we conducted an epidemiological survey of HepVs carried by small terrestrial mammals in the wild in Yunnan Province, China. Utilizing HepV-specific broad-spectrum RT-PCR, next-generation sequencing (NGS), and QNome nanopore sequencing (QNS) techniques, we identified and characterized two novel HepVs provisionally named EpMa-HAV and EpLe-HAV, discovered in the long-tailed mountain shrew ( Episoriculus macrurus ) and long-tailed brown-toothed shrew ( Episoriculus leucops ), respectively. Our sequence and phylogenetic analyses of EpMa-HAV and EpLe-HAV indicated that they belong to the species Hepatovirus I (HepV-I) clade II, also known as the Chinese shrew HepV clade. Notably, the codon usage bias pattern of novel shrew HepVs is consistent with that of previously identified Chinese shrew HepV. Furthermore, our structural analysis demonstrated that shrew HepVs differ from other mammalian HepVs in RNA secondary structure and exhibit variances in key protein sites. Overall, the discovery of two novel HepVs in shrews expands the host range of HepV and underscores the existence of genetically diverse animal homologs of human HAV within the genus HepV.
- Published
- 2024
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3. A SARS-CoV-2-Related Virus from Malayan Pangolin Causes Lung Infection without Severe Disease in Human ACE2-Transgenic Mice.
- Author
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Liu MQ, Lin HF, Li J, Chen Y, Luo Y, Zhang W, Hu B, Tian FJ, Hu YJ, Liu YJ, Jiang RD, Gong QC, Li A, Guo ZS, Li B, Yang XL, Tong YG, and Shi ZL
- Subjects
- Animals, Humans, Mice, Angiotensin-Converting Enzyme 2 genetics, Cell Line, China, Lung pathology, Lung virology, Mice, Transgenic, SARS-CoV-2 classification, SARS-CoV-2 genetics, SARS-CoV-2 pathogenicity, Swine, Chiroptera, COVID-19 transmission, COVID-19 virology, Host Specificity, Pangolins virology
- Abstract
Coronavirus disease 2019 (COVID-19), which is caused by the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the most severe emerging infectious disease in the current century. The discovery of SARS-CoV-2-related coronaviruses (SARSr-CoV-2) in bats and pangolins in South Asian countries indicates that SARS-CoV-2 likely originated from wildlife. To date, two SARSr-CoV-2 strains have been isolated from pangolins seized in Guangxi and Guangdong by the customs agency of China, respectively. However, it remains unclear whether these viruses cause disease in animal models and whether they pose a transmission risk to humans. In this study, we investigated the biological features of a SARSr-CoV-2 strain isolated from a smuggled Malayan pangolin (Manis javanica) captured by the Guangxi customs agency, termed MpCoV-GX, in terms of receptor usage, cell tropism, and pathogenicity in wild-type BALB/c mice, human angiotensin-converting enzyme 2 (ACE2)-transgenic mice, and human ACE2 knock-in mice. We found that MpCoV-GX can utilize ACE2 from humans, pangolins, civets, bats, pigs, and mice for cell entry and infect cell lines derived from humans, monkeys, bats, minks, and pigs. The virus could infect three mouse models but showed limited pathogenicity, with mild peribronchial and perivascular inflammatory cell infiltration observed in lungs. Our results suggest that this SARSr-CoV-2 virus from pangolins has the potential for interspecies infection, but its pathogenicity is mild in mice. Future surveillance among these wildlife hosts of SARSr-CoV-2 is needed to monitor variants that may have higher pathogenicity and higher spillover risk. IMPORTANCE SARS-CoV-2, which likely spilled over from wildlife, is the third highly pathogenic human coronavirus. Being highly transmissible, it is perpetuating a pandemic and continuously posing a severe threat to global public health. Several SARS-CoV-2-related coronaviruses (SARSr-CoV-2) in bats and pangolins have been identified since the SARS-CoV-2 outbreak. It is therefore important to assess their potential of crossing species barriers for better understanding of their risk of future emergence. In this work, we investigated the biological features and pathogenicity of a SARSr-CoV-2 strain isolated from a smuggled Malayan pangolin, named MpCoV-GX. We found that MpCoV-GX can utilize ACE2 from 7 species for cell entry and infect cell lines derived from a variety of mammalian species. MpCoV-GX can infect mice expressing human ACE2 without causing severe disease. These findings suggest the potential of cross-species transmission of MpCoV-GX, and highlight the need of further surveillance of SARSr-CoV-2 in pangolins and other potential animal hosts.
- Published
- 2023
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4. Ecological study of cave nectar bats reveals low risk of direct transmission of bat viruses to humans.
- Author
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Zhao K, Zhang W, Li B, Xie SZ, Yi F, Jiang RD, Luo Y, He XY, Zhang YZ, Shi ZL, Zhang LB, and Yang XL
- Subjects
- Animals, China epidemiology, Humans, Phylogeny, Plant Nectar, Chiroptera, Coronavirus Infections veterinary, Viruses
- Abstract
Bats are reservoirs of various viruses. The widely distributed cave nectar bat ( Eonycteris spelaea ) is known to carry both filoviruses and coronaviruses. However, the potential transmission of theses bat viruses to humans is not fully understood. In this study, we tracked 16 E. spelaea bats in Mengla County, Yunnan Province, China, using miniaturized GPS devices to investigate their movements and potential contact with humans. Furthermore, to determine the prevalence of coronavirus and filovirus infections, we screened for the nucleic acids of the Měnglà virus (MLAV) and two coronaviruses (GCCDC1-CoV and HKU9-CoV) in anal swab samples taken from bats and for antibodies against these viruses in human serum samples. None of the serum samples were found to contain antibodies against the bat viruses. The GPS tracking results showed that the bats did not fly during the daytime and rarely flew to residential areas. The foraging range of individual bats also varied, with a mean cumulative nightly flight distance of 25.50 km and flight speed of up to 57.4 km/h. Taken together, these results suggest that the risk of direct transmission of GCCDC1-CoV, HKU9-CoV, and MLAV from E. spelaea bats to humans is very low under natural conditions.
- Published
- 2022
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5. Genomic Characterization of Diverse Bat Coronavirus HKU10 in Hipposideros Bats.
- Author
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Wang N, Luo CM, Yang XL, Liu HZ, Zhang LB, Zhang W, Li B, Zhu Y, Peng C, Shi ZL, and Hu B
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- Alphacoronavirus pathogenicity, Animals, Base Sequence genetics, Biological Evolution, China, Chiroptera genetics, Coronavirus genetics, Coronavirus pathogenicity, Coronavirus Infections virology, Evolution, Molecular, Genetic Variation genetics, Genome, Viral genetics, Genotype, Phylogeny, Sequence Analysis, DNA methods, Viral Proteins genetics, Alphacoronavirus genetics, Chiroptera virology
- Abstract
Bats have been identified as natural reservoirs of a variety of coronaviruses. They harbor at least 19 of the 33 defined species of alpha- and betacoronaviruses. Previously, the bat coronavirus HKU10 was found in two bat species of different suborders, Rousettus leschenaultia and Hipposideros pomona , in south China. However, its geographic distribution and evolution history are not fully investigated. Here, we screened this viral species by a nested reverse transcriptase PCR in our archived samples collected over 10 years from 25 provinces of China and one province of Laos. From 8004 bat fecal samples, 26 were found to be positive for bat coronavirus HKU10 (BtCoV HKU10). New habitats of BtCoV HKU10 were found in the Yunnan, Guangxi, and Hainan Provinces of China, and Louang Namtha Province in Laos. In addition to H . pomona , BtCoV HKU10 variants were found circulating in Aselliscus stoliczkanus and Hipposideros larvatus . We sequenced full-length genomes of 17 newly discovered BtCoV HKU10 strains and compared them with previously published sequences. Our results revealed a much higher genetic diversity of BtCoV HKU10, particularly in spike genes and accessory genes. Besides the two previously reported lineages, we found six novel lineages in their new habitats, three of which were located in Yunnan province. The genotypes of these viruses are closely related to sampling locations based on polyproteins, and correlated to bat species based on spike genes. Combining phylogenetic analysis, selective pressure, and molecular-clock calculation, we demonstrated that Yunnan bats harbor a gene pool of BtCoV HKU10, with H . pomona as a natural reservoir. The cell tropism test using spike-pseudotyped lentivirus system showed that BtCoV HKU10 could enter cells from human and bat, suggesting a potential interspecies spillover. Continuous studies on these bat coronaviruses will expand our understanding of the evolution and genetic diversity of coronaviruses, and provide a prewarning of potential zoonotic diseases from bats.
- Published
- 2021
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6. A pneumonia outbreak associated with a new coronavirus of probable bat origin.
- Author
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Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, Chen HD, Chen J, Luo Y, Guo H, Jiang RD, Liu MQ, Chen Y, Shen XR, Wang X, Zheng XS, Zhao K, Chen QJ, Deng F, Liu LL, Yan B, Zhan FX, Wang YY, Xiao GF, and Shi ZL
- Subjects
- Angiotensin-Converting Enzyme 2, Animals, Antibodies, Viral blood, Betacoronavirus metabolism, Betacoronavirus ultrastructure, COVID-19, Cell Line, China epidemiology, Chlorocebus aethiops, Female, Genome, Viral genetics, Humans, Male, Peptidyl-Dipeptidase A metabolism, Phylogeny, Severe acute respiratory syndrome-related coronavirus classification, Severe acute respiratory syndrome-related coronavirus genetics, SARS-CoV-2, Sequence Homology, Nucleic Acid, Severe Acute Respiratory Syndrome, Vero Cells, Betacoronavirus classification, Betacoronavirus genetics, Chiroptera virology, Coronavirus Infections epidemiology, Coronavirus Infections virology, Disease Outbreaks, Pneumonia, Viral epidemiology, Pneumonia, Viral virology
- Abstract
Since the outbreak of severe acute respiratory syndrome (SARS) 18 years ago, a large number of SARS-related coronaviruses (SARSr-CoVs) have been discovered in their natural reservoir host, bats
1-4 . Previous studies have shown that some bat SARSr-CoVs have the potential to infect humans5-7 . Here we report the identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China. The epidemic, which started on 12 December 2019, had caused 2,794 laboratory-confirmed infections including 80 deaths by 26 January 2020. Full-length genome sequences were obtained from five patients at an early stage of the outbreak. The sequences are almost identical and share 79.6% sequence identity to SARS-CoV. Furthermore, we show that 2019-nCoV is 96% identical at the whole-genome level to a bat coronavirus. Pairwise protein sequence analysis of seven conserved non-structural proteins domains show that this virus belongs to the species of SARSr-CoV. In addition, 2019-nCoV virus isolated from the bronchoalveolar lavage fluid of a critically ill patient could be neutralized by sera from several patients. Notably, we confirmed that 2019-nCoV uses the same cell entry receptor-angiotensin converting enzyme II (ACE2)-as SARS-CoV.- Published
- 2020
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7. Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes.
- Author
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Zhang W, Du RH, Li B, Zheng XS, Yang XL, Hu B, Wang YY, Xiao GF, Yan B, Shi ZL, and Zhou P
- Subjects
- COVID-19, China, Coronavirus Infections blood, Humans, Pneumonia, Viral blood, SARS-CoV-2, Betacoronavirus isolation & purification, Coronavirus Infections transmission, Feces virology, Pneumonia, Viral transmission, Virus Shedding
- Abstract
In December 2019, a novel coronavirus (2019-nCoV) caused an outbreak in Wuhan, China, and soon spread to other parts of the world. It was believed that 2019-nCoV was transmitted through respiratory tract and then induced pneumonia, thus molecular diagnosis based on oral swabs was used for confirmation of this disease. Likewise, patient will be released upon two times of negative detection from oral swabs. However, many coronaviruses can also be transmitted through oral-fecal route by infecting intestines. Whether 2019-nCoV infected patients also carry virus in other organs like intestine need to be tested. We conducted investigation on patients in a local hospital who were infected with this virus. We found the presence of 2019-nCoV in anal swabs and blood as well, and more anal swab positives than oral swab positives in a later stage of infection, suggesting shedding and thereby transmitted through oral-fecal route. We also showed serology test can improve detection positive rate thus should be used in future epidemiology. Our report provides a cautionary warning that 2019-nCoV may be shed through multiple routes.
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- 2020
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8. Coexistence of multiple coronaviruses in several bat colonies in an abandoned mineshaft.
- Author
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Ge XY, Wang N, Zhang W, Hu B, Li B, Zhang YZ, Zhou JH, Luo CM, Yang XL, Wu LJ, Wang B, Zhang Y, Li ZX, and Shi ZL
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, China epidemiology, Coronavirus genetics, Coronavirus Infections epidemiology, Coronavirus Infections veterinary, Genome, Viral, Phylogeny, Polymerase Chain Reaction methods, RNA, Viral genetics, RNA-Dependent RNA Polymerase genetics, Severe acute respiratory syndrome-related coronavirus genetics, Severe acute respiratory syndrome-related coronavirus isolation & purification, Sequence Analysis, DNA, Severe Acute Respiratory Syndrome epidemiology, Severe Acute Respiratory Syndrome genetics, Severe Acute Respiratory Syndrome veterinary, Chiroptera virology, Coronavirus isolation & purification, Coronavirus Infections virology
- Abstract
Since the 2002-2003 severe acute respiratory syndrome (SARS) outbreak prompted a search for the natural reservoir of the SARS coronavirus, numerous alpha- and betacoronaviruses have been discovered in bats around the world. Bats are likely the natural reservoir of alpha- and betacoronaviruses, and due to the rich diversity and global distribution of bats, the number of bat coronaviruses will likely increase. We conducted a surveillance of coronaviruses in bats in an abandoned mineshaft in Mojiang County, Yunnan Province, China, from 2012-2013. Six bat species were frequently detected in the cave: Rhinolophus sinicus, Rhinolophus affinis, Hipposideros pomona, Miniopterus schreibersii, Miniopterus fuliginosus, and Miniopterus fuscus. By sequencing PCR products of the coronavirus RNA-dependent RNA polymerase gene (RdRp), we found a high frequency of infection by a diverse group of coronaviruses in different bat species in the mineshaft. Sequenced partial RdRp fragments had 80%-99% nucleic acid sequence identity with well-characterized Alphacoronavirus species, including BtCoV HKU2, BtCoV HKU8, and BtCoV1, and unassigned species BtCoV HKU7 and BtCoV HKU10. Additionally, the surveillance identified two unclassified betacoronaviruses, one new strain of SARS-like coronavirus, and one potentially new betacoronavirus species. Furthermore, coronavirus co-infection was detected in all six bat species, a phenomenon that fosters recombination and promotes the emergence of novel virus strains. Our findings highlight the importance of bats as natural reservoirs of coronaviruses and the potentially zoonotic source of viral pathogens.
- Published
- 2016
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9. Isolation and identification of bat viruses closely related to human, porcine and mink orthoreoviruses.
- Author
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Yang XL, Tan B, Wang B, Li W, Wang N, Luo CM, Wang MN, Zhang W, Li B, Peng C, Ge XY, Zhang LB, and Shi ZL
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- Animals, China epidemiology, Disease Reservoirs virology, Humans, Orthoreovirus, Mammalian genetics, Phylogeny, Reoviridae Infections epidemiology, Reoviridae Infections transmission, Reoviridae Infections virology, Serogroup, Chiroptera virology, Mink virology, Orthoreovirus, Mammalian classification, Orthoreovirus, Mammalian isolation & purification, Reoviridae Infections veterinary, Swine virology
- Abstract
Bats have been identified as natural reservoirs of many viruses, including reoviruses. Recent studies have demonstrated the interspecies transmission of bat reoviruses to humans. In this study, we report the isolation and molecular characterization of six strains of mammalian orthoreovirus (MRV) from Hipposideros and Myotis spp. These isolates were grouped into MRV serotype 1, 2 or 3 based on the sequences of the S1 gene, which encodes the outer coat protein s1. Importantly, we found that three of six bat MRV strains shared high similarity with MRVs isolated from diseased minks, piglets or humans based on the S1 segment, suggesting that interspecies transmission has occurred between bats and humans or animals. Phylogenetic analyses based on the 10 segments showed that the genomic segments of these bat MRVs had different evolution lineages, suggesting that these bat MRVs may have arisen through reassortment of MRVs of different origins.
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- 2015
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10. Genetic diversity and temporal dynamics of phytoplankton viruses in East Lake, China.
- Author
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Wang MN, Ge XY, Wu YQ, Yang XL, Tan B, Zhang YJ, and Shi ZL
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- Biota, China, Cluster Analysis, Lakes, Molecular Sequence Data, Phylogeny, Plant Viruses genetics, Population Dynamics, Seasons, Sequence Analysis, DNA, Sequence Homology, Genetic Variation, Phytoplankton virology, Plant Viruses classification, Plant Viruses isolation & purification
- Abstract
Phytoplankton viruses are important components of aquatic ecosystems. However, their prevalence and genetic diversity in marine and freshwater systems are largely under estimated owing to the immense size of water bodies and limitations in virus discovery techniques. In this study, we conducted a 1-year survey of phytoplankton virus communities by collecting surface water monthly from an inland lake (East Lake) in China between May 2012 and April 2013. We examined four phytoplankton viruses, i.e., myoviruses, podoviruses, siphoviruses, and phycodnaviruses, and seven sets of primers were used to target conserved genes within these four species. In this year-long investigation, a total of 358 different virus-related sequences from four virus families were obtained. All virus families were detected in all months, except for cyanopodoviruses, which were only identified during eight of the 12 months surveyed. Moreover, virus abundance and diversity changed dynamically over time. Phylogenetic analysis revealed that the majority of viral sequences from East Lake, China displayed distinct clustering patterns compared with published sequences. These results supported the existence of a highly diverse and unique phytoplankton virus community in East Lake, China.
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- 2015
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11. Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.
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Ge XY, Li JL, Yang XL, Chmura AA, Zhu G, Epstein JH, Mazet JK, Hu B, Zhang W, Peng C, Zhang YJ, Luo CM, Tan B, Wang N, Zhu Y, Crameri G, Zhang SY, Wang LF, Daszak P, and Shi ZL
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- Angiotensin-Converting Enzyme 2, Animals, China, Chlorocebus aethiops, Disease Reservoirs virology, Feces virology, Fluorescent Antibody Technique, Genome, Viral genetics, Host Specificity, Humans, Molecular Sequence Data, Pandemics prevention & control, Pandemics veterinary, Peptidyl-Dipeptidase A genetics, Real-Time Polymerase Chain Reaction, Receptors, Virus genetics, Receptors, Virus metabolism, Severe acute respiratory syndrome-related coronavirus genetics, Severe acute respiratory syndrome-related coronavirus ultrastructure, Severe Acute Respiratory Syndrome prevention & control, Severe Acute Respiratory Syndrome transmission, Severe Acute Respiratory Syndrome veterinary, Severe Acute Respiratory Syndrome virology, Species Specificity, Spike Glycoprotein, Coronavirus chemistry, Spike Glycoprotein, Coronavirus metabolism, Vero Cells, Virion isolation & purification, Virion ultrastructure, Virus Internalization, Viverridae metabolism, Chiroptera virology, Peptidyl-Dipeptidase A metabolism, Severe acute respiratory syndrome-related coronavirus isolation & purification, Severe acute respiratory syndrome-related coronavirus metabolism
- Abstract
The 2002-3 pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV) was one of the most significant public health events in recent history. An ongoing outbreak of Middle East respiratory syndrome coronavirus suggests that this group of viruses remains a key threat and that their distribution is wider than previously recognized. Although bats have been suggested to be the natural reservoirs of both viruses, attempts to isolate the progenitor virus of SARS-CoV from bats have been unsuccessful. Diverse SARS-like coronaviruses (SL-CoVs) have now been reported from bats in China, Europe and Africa, but none is considered a direct progenitor of SARS-CoV because of their phylogenetic disparity from this virus and the inability of their spike proteins to use the SARS-CoV cellular receptor molecule, the human angiotensin converting enzyme II (ACE2). Here we report whole-genome sequences of two novel bat coronaviruses from Chinese horseshoe bats (family: Rhinolophidae) in Yunnan, China: RsSHC014 and Rs3367. These viruses are far more closely related to SARS-CoV than any previously identified bat coronaviruses, particularly in the receptor binding domain of the spike protein. Most importantly, we report the first recorded isolation of a live SL-CoV (bat SL-CoV-WIV1) from bat faecal samples in Vero E6 cells, which has typical coronavirus morphology, 99.9% sequence identity to Rs3367 and uses ACE2 from humans, civets and Chinese horseshoe bats for cell entry. Preliminary in vitro testing indicates that WIV1 also has a broad species tropism. Our results provide the strongest evidence to date that Chinese horseshoe bats are natural reservoirs of SARS-CoV, and that intermediate hosts may not be necessary for direct human infection by some bat SL-CoVs. They also highlight the importance of pathogen-discovery programs targeting high-risk wildlife groups in emerging disease hotspots as a strategy for pandemic preparedness.
- Published
- 2013
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